scholarly journals Cytochrome P450 CYP81A12 and CYP81A21 Are Associated with Resistance to Two Acetolactate Synthase Inhibitors in Echinochloa phyllopogon

2014 ◽  
Vol 165 (2) ◽  
pp. 618-629 ◽  
Author(s):  
Satoshi Iwakami ◽  
Masaki Endo ◽  
Hiroaki Saika ◽  
Junichi Okuno ◽  
Naoki Nakamura ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Acetolactate Synthase ◽  
Echinochloa Phyllopogon

Functional characterization of cytochrome P450 CYP81A subfamily to disclose the pattern of cross-resistance in Echinochloa phyllopogon

2020 ◽  
Vol 102 (4-5) ◽  
pp. 403-416 ◽  
Author(s):  
Niña Gracel Dimaano ◽  
Takuya Yamaguchi ◽  
Kanade Fukunishi ◽  
Tohru Tominaga ◽  
Satoshi Iwakami
Keyword(s):  
Cytochrome P450 ◽  
Functional Characterization ◽  
Cross Resistance ◽  
Echinochloa Phyllopogon

scholarly journals Resistance Mechanism to Metsulfuron-Methyl in Polypogon fugax

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1309
Author(s):  
Xiaoyue Yu ◽  
Hanwen Wu ◽  
Jianping Zhang ◽  
Yongjie Yang ◽  
Wei Tang ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Sequence Analysis ◽  
Synergistic Effect ◽  
Enzymatic Activity ◽  
Resistance Mechanism ◽  
Acetolactate Synthase ◽  
Metsulfuron Methyl ◽  
Herbicide Metabolism ◽  
Glutathione S Transferases ◽  
Gst Activity

Polypogon fugax is a common winter weed in China and other Asia countries. We have previously found a P. fugax biotype (R) resistant to acetyl co-enzyme A carboxylase (ACCase) herbicides also cannot be effectively controlled by some acetolactate synthase (ALS) herbicides. This study evaluated the level of resistance to four ALS herbicides (metsulfuron-methyl, chlorsulfuron, monosulfuron, pyribambenz isopropyl) in the R biotype and the associated resistance mechanism. The R biotype exhibited moderate level of resistance to metsulfuron-methyl (6.0-fold) compared with the sensitive biotype (S). Sequence analysis of ALS gene revealed that two ALS genes existed in P. fugax. However, no substitution associated with ALS resistance mechanism were found in ALS genes between the S and R biotypes. The activity of ALS enzyme isolated from the R biotype was inherently higher and less sensitive to metsulfuron-methyl than the S biotype. Glutathione S-transferases (GST) activity was also less sensitive to metsulfuron-methyl in the R than as the S biotypes. Malathion, a cytochrome P450 (CYP) monooxygenase inhibitor, had much greater synergistic effect with metsulfuron-methyl on the R than as the S plants, reducing the ED50 value (herbicide dose to inhibit growth by 50%) of metsulfuron-methyl by 23- and 6-fold, respectively, suggesting that CYP mediated enhanced metabolism might contribute to the resistance to ALS herbicides. These results suggest that metsulfuron-methyl resistance in the R biotype was associated with the up-regulated ALS enzymatic activity and the GST and CYP-mediated enhanced herbicide metabolism.

Isolation and expression of genes for acetolactate synthase and acetyl-CoA carboxylase in Echinochloa phyllopogon, a polyploid weed species

2012 ◽  
Vol 68 (7) ◽  
pp. 1098-1106 ◽  
Author(s):  
Satoshi Iwakami ◽  
Akira Uchino ◽  
Hiroaki Watanabe ◽  
Yuji Yamasue ◽  
Tatsuya Inamura
Keyword(s):  
Acetolactate Synthase ◽  
Acetyl Coa Carboxylase ◽  
Weed Species ◽  
Acetyl Coa ◽  
Expression Of Genes ◽  
Echinochloa Phyllopogon

scholarly journals A Novel Rice Cytochrome P450 Gene, CYP72A31, Confers Tolerance to Acetolactate Synthase-Inhibiting Herbicides in Rice and Arabidopsis

2014 ◽  
Vol 166 (3) ◽  
pp. 1232-1240 ◽  
Author(s):  
H. Saika ◽  
J. Horita ◽  
F. Taguchi-Shiobara ◽  
S. Nonaka ◽  
A. Nishizawa-Yokoi ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Acetolactate Synthase ◽  
Cytochrome P450 Gene ◽  
P450 Gene

Cytochrome P450 genes induced by bispyribac-sodium treatment in a multiple-herbicide-resistant biotype of Echinochloa phyllopogon

2013 ◽  
Vol 70 (4) ◽  
pp. 549-558 ◽  
Author(s):  
Satoshi Iwakami ◽  
Akira Uchino ◽  
Yukiko Kataoka ◽  
Hiroyuki Shibaike ◽  
Hiroaki Watanabe ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Herbicide Resistant ◽  
Cytochrome P450 Genes ◽  
Echinochloa Phyllopogon

scholarly journals Resistance ofEchinochloa crus-galliPopulations to Acetolactate Synthase-Inhibiting Herbicides

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Dilpreet S. Riar ◽  
Jason K. Norsworthy ◽  
Jason A. Bond ◽  
Mohammad T. Bararpour ◽  
Michael J. Wilson ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Acetolactate Synthase ◽  
Dry Weight ◽  
Cytochrome P450 Monooxygenase ◽  
Rice Fields ◽  
Cross Resistance ◽  
P450 Monooxygenase ◽  
Susceptible Population ◽  
Herbicide Degradation ◽  
Mechanism Of Resistance

ThreeEchinochloa crus-galli(barnyardgrass) populations from rice fields in Arkansas (AR1 and AR2) and Mississippi (MS1), USA, were recently confirmed to be resistant to imazethapyr. Experiments were conducted to characterize cross-resistance to acetolactate synthase- (ALS-) inhibiting herbicides and determine if malathion, a known cytochrome P450 monooxygenase (CYP) inhibitor, would overcome resistance. The AR1 and MS1 populations were cross-resistant to bispyribac-sodium; however, AR2 was sensitive to bispyribac-sodium. The AR1, AR2, and MS1 populations were >94, >94, and 3.3 times, respectively, more resistant to imazamox; >94, 30, and 9.4 times, respectively, more resistant to penoxsulam; and 15, 0.9, and 7.2 times, respectively, more resistant to bispyribac-sodium compared to a susceptible population. Addition of malathion to penoxsulam reduced dry weight of all populations and increased mortality of AR2 and MS1 populations compared to penoxsulam alone. Addition of malathion to imazethapyr and bispyribac-sodium increased the mortality of MS1 population in mixture with imazethapyr and AR1 population in mixture with bispyribac-sodium compared to treatments with imazethapyr and bispyribac-sodium applied alone. Synergism of ALS-inhibiting herbicides with malathion indicates increased herbicide degradation by CYP as partial mechanism of resistance to penoxsulam in all resistant populations and probably to imazethapyr in MS1 and bispyribac-sodium in AR1 populations.

Target site–based resistance to penoxsulam in late watergrass (Echinochloa phyllopogon) from China

Weed Science ◽  
2019 ◽  
Vol 67 (4) ◽  
pp. 380-388 ◽  
Author(s):  
Jian Liu ◽  
Jiapeng Fang ◽  
Zongzhe He ◽  
Jun Li ◽  
Liyao Dong
Keyword(s):  
Binding Affinity ◽  
Acetolactate Synthase ◽  
Resistance Mechanisms ◽  
Target Site ◽  
Future Research ◽  
Target Site Resistance ◽  
Target Gene Sequence ◽  
Als Inhibitors ◽  
Echinochloa Phyllopogon

AbstractLate watergrass [Echinochloa phyllopogon (Stapf) Koso-Pol.] is one of the most persistent weeds in rice fields and shows resistance to some acetolactate synthase (ALS)-inhibiting herbicides, such as penoxsulam. Previous studies of E. phyllopogon’s herbicide resistance have focused on non–target site resistance mechanisms. In this study, E. phyllopogon populations from Heilong Jiang Province, China, that were possibly resistant to penoxsulam were used to identify the target site–based mechanisms of resistance. Population HSRH-520 showed a 25.4-fold higher resistance to penoxsulam than the sensitive population, HSRH-538. HSRH-520 was resistant to other ALS inhibitors, with resistance indexes ranging from 17.1 to 166. Target-gene sequence analysis revealed two different ALS genes in E. phyllopogon; a Pro-197-Ser substitution occurred in the ALS-2 gene of HSRH-520. In vitro activity assays revealed that the penoxsulam concentrations required to inhibit 50% of the ALS activity were 13.7 times higher in HSRH-520 than in HSRH-538. Molecular-docking tests showed that the Pro-197-Ser mutation reduced the binding affinity between ALS and ALS inhibitors belonging to the triazolopyrimidine, sulfonylaminocarbonyltriazolinone, and sulfonylurea families, and there were almost no effects on binding affinity when the ALS inhibitors were of the pyrimidinylthiobenzoate and imidazolinone families. Overall, the results indicated and verified that the Pro-197-Ser mutation leads to increased ALS activity by reducing the binding affinity of the inhibitor and ALS. This is the first report on the Pro-197-Ser mutation in the complete ALS gene of E. phyllopogon and will aid future research of target site–based resistance mechanisms of E. phyllopogon to ALS inhibitors.

Molecular evolution of a cytochrome P450 for the synthesis of potential antidepressant (2R,6R)-hydroxynorketamine

2021 ◽  
Author(s):  
Ansgar Bokel ◽  
Michael C. Hutter ◽  
Vlada B. Urlacher
Keyword(s):  
Cytochrome P450 ◽  
Molecular Evolution ◽  
Cytochrome P450 Monooxygenase ◽  
P450 Monooxygenase ◽  
Effective Synthesis

Engineered cytochrome P450 monooxygenase CYP154E1 enables the effective synthesis of the potential antidepressant (2R,6R)-hydroxynorketamine via N-demethylation and regio- and stereoselective hydroxylation of (R)-ketamine.

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