Isolation and expression of genes for acetolactate synthase and acetyl-CoA carboxylase in Echinochloa phyllopogon, a polyploid weed species

Satoshi Iwakami; Akira Uchino; Hiroaki Watanabe; Yuji Yamasue; Tatsuya Inamura

doi:10.1002/ps.3287

Expression of genes encoding acetyl-CoA carboxylase, biotin synthase, and acetyl-CoA generating enzymes in Arabidopsis thaliana

10.31274/rtd-180813-13275 ◽

1997 ◽

Author(s):

Jinshan Ke

Keyword(s):

Arabidopsis Thaliana ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Biotin Synthase ◽

Expression Of Genes ◽

Genes Encoding

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Phosphorylation of Acetyl-CoA Carboxylase by AMPK Reduces Renal Fibrosis and Is Essential for the Anti-Fibrotic Effect of Metformin

Journal of the American Society of Nephrology ◽

10.1681/asn.2018010050 ◽

2018 ◽

Vol 29 (9) ◽

pp. 2326-2336 ◽

Cited By ~ 27

Author(s):

Mardiana Lee ◽

Marina Katerelos ◽

Kurt Gleich ◽

Sandra Galic ◽

Bruce E. Kemp ◽

...

Keyword(s):

Fatty Acid ◽

Epithelial Cells ◽

Lipid Accumulation ◽

Acid Oxidation ◽

Acetyl Coa Carboxylase ◽

Tubular Epithelial Cells ◽

Acetyl Coa ◽

Expression Of Genes ◽

Energy Sensing ◽

Unilateral Ureteric Obstruction

BackgroundExpression of genes regulating fatty acid metabolism is reduced in tubular epithelial cells from kidneys with tubulointerstitial fibrosis (TIF), thus decreasing the energy produced by fatty acid oxidation (FAO). Acetyl-CoA carboxylase (ACC), a target for the energy-sensing AMP-activating protein kinase (AMPK), is the major controller of the rate of FAO within cells. Metformin has a well described antifibrotic effect, and increases phosphorylation of ACC by AMPK, thereby increasing FAO.MethodsWe evaluated phosphorylation of ACC in cell and mouse nephropathy models, as well as the effects of metformin administration in mice with and without mutations that reduce ACC phosphorylation.ResultsReduced phosphorylation of ACC on the AMPK site Ser79 occurred in both tubular epithelial cells treated with folate to mimic cellular injury and in wild-type (WT) mice after induction of the folic acid nephropathy model. When this effect was exaggerated in mice with knock-in (KI) Ser to Ala mutations of the phosphorylation sites in ACC, lipid accumulation and fibrosis increased significantly compared with WT. The effect of ACC phosphorylation on fibrosis was confirmed in the unilateral ureteric obstruction model, which showed significantly increased lipid accumulation and fibrosis in the KI mice. Metformin use was associated with significantly reduced fibrosis and lipid accumulation in WT mice. In contrast, in the KI mice, the drug was associated with worsened fibrosis.ConclusionsThese data indicate that reduced phosphorylation of ACC after renal injury contributes to the development of TIF, and that phosphorylation of ACC is required for metformin’s antifibrotic action in the kidney.

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CYP81A P450s are involved in concomitant cross‐resistance to acetolactate synthase and acetyl‐CoA carboxylase herbicides inEchinochloa phyllopogon

New Phytologist ◽

10.1111/nph.15552 ◽

2018 ◽

Vol 221 (4) ◽

pp. 2112-2122 ◽

Cited By ~ 21

Author(s):

Satoshi Iwakami ◽

Yoshitaka Kamidate ◽

Takuya Yamaguchi ◽

Masumi Ishizaka ◽

Masaki Endo ◽

...

Keyword(s):

Acetolactate Synthase ◽

Cross Resistance ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa

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Resistance to Acetolactate Synthase and Acetyl Co-A Carboxylase Inhibitors in North Carolina Italian Ryegrass (Lolium perenne)

Weed Technology ◽

10.1614/wt-d-11-00050.1 ◽

2011 ◽

Vol 25 (4) ◽

pp. 659-666 ◽

Cited By ~ 10

Author(s):

Aman Chandi ◽

Alan C. York ◽

David L. Jordan ◽

Josh B. Beam

Keyword(s):

North Carolina ◽

Lolium Perenne ◽

Acetolactate Synthase ◽

Italian Ryegrass ◽

Cross Resistance ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Als Inhibitors

Diclofop-resistant Italian ryegrass is widespread in southwestern North Carolina, and growers have resorted to using acetolactate synthase (ALS) inhibitors such as mesosulfuron and pyroxsulam to control this weed in wheat. In the spring of 2007, mesosulfuron failed to control Italian ryegrass in several wheat fields. Seed were collected from six fields in two counties and greenhouse studies were conducted to determine response to mesosulfuron and the acetyl-CoA carboxylase (ACCase) inhibitors diclofop and pinoxaden. All populations were resistant to diclofop and cross-resistant to pinoxaden. Five of the six populations were resistant to diclofop, pinoxaden, and mesosulfuron. An additional study with two biotypes confirmed cross-resistance to the ALS inhibitors imazamox, mesosulfuron, and pyroxsulam. Resistance to mesosulfuron was heritable.

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First Detection and Characterization of Cross- and Multiple Resistance to Acetyl-CoA Carboxylase (ACCase)- and Acetolactate Synthase (ALS)-Inhibiting Herbicides in Black-Grass (Alopecurus myosuroides) and Italian Ryegrass (Lolium multiflorum) Populations from Ireland

Agriculture ◽

10.3390/agriculture11121272 ◽

2021 ◽

Vol 11 (12) ◽

pp. 1272

Author(s):

Vijaya Bhaskar Alwarnaidu Vijayarajan ◽

Patrick D Forristal ◽

Sarah K Cook ◽

David Schilder ◽

Jimmy Staples ◽

...

Keyword(s):

Lolium Multiflorum ◽

Acetolactate Synthase ◽

Target Site ◽

Italian Ryegrass ◽

Integrated Weed Management ◽

Multiple Resistance ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Alopecurus Myosuroides ◽

Target Site Resistance

Understanding the resistance spectrum and underlying genetic mechanisms is critical for managing herbicide-resistant populations. In this study, resistance to acetyl CoA carboxylase (ACCase) and acetolactate synthase (ALS) inhibitors was investigated in four suspected resistant populations of Alopecurus myosuroides (ALOMY-001 to ALOMY-004) and Lolium multiflorum (LOLMU-001 to LOLMU-004), collected from cereal production fields in Ireland. Glasshouse assays with three ALOMY-active herbicides [propaquizafop, cycloxydim (ACCase) and mesosulfuron + iodosulfuron (ALS)] or five LOLMU-active herbicides [pinoxaden, propaquizafop, cycloxydim (ACCase) and mesosulfuron + iodosulfuron, pyroxsulam (ALS)], and target-site resistance mechanism studies, based on pyrosequencing, were carried out in each of those populations. For A. myosuroides, Ile-1781-Leu ACCase mutation contributed to propaquizafop and cycloxydim resistance (shoot dry weight GR50 resistance factor (RF) = 7.5–35.5) in all ALOMY populations, and the independent Pro-197-Thr or Pro-197-Ser ALS mutation contributed to mesosulfuron + iodosulfuron resistance (RF = 3.6–6.6), in ALOMY-002 to ALOMY-004. Most of the analyzed plants for these mutations were homo/heterozygous combinations or only heterozygous. For L. multiflorum, phenotypic resistance to mesosulfuron + iodosulfuron (RF = 11.9–14.6) and pyroxsulam (RF = 2.3–3.1) was noted in all LOLMU populations, but the Pro-197-Gln or Pro-197-Leu ALS mutation (mostly in homozygous status) was identified in LOLMU-001, LOLMU-002 and LOLMU-004 only. Additionally, despite no known ACCase mutations in any LOLMU populations, LOLMU-002 survived pinoxaden and propaquizafop application (RF = 3.4 or 1.3), and LOLMU-003 survived pinoxaden (RF = 2.3), suggesting the possibility of non-target-site resistance mechanisms for ACCase and/or ALS resistance in these populations. Different resistance levels, as evidenced by a reduction in growth as dose increased above field rates in ALOMY and LOLMU, were due to variations in mutation rate and the level of heterozygosity, resulting in an overall resistance rating of low to moderate. This is the first study confirming cross- and multiple resistance to ACCase- and ALS-inhibiting herbicides, highlighting that resistance monitoring in A. myosuroides and L. multiflorum in Ireland is critical, and the adoption of integrated weed management strategies (chemical and non-chemical/cultural strategies) is essential.

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Mesosulfuron-Resistant Italian Ryegrass (Lolium Multiflorum) Biotype from Texas

Weed Technology ◽

10.1614/wt-08-032.1 ◽

2008 ◽

Vol 22 (3) ◽

pp. 431-434 ◽

Cited By ~ 9

Author(s):

Andrew T. Ellis ◽

Gaylon D. Morgan ◽

Thomas C. Mueller

Keyword(s):

Winter Wheat ◽

Plant Biomass ◽

Lolium Multiflorum ◽

Acetolactate Synthase ◽

Italian Ryegrass ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Resistance Level ◽

Greenhouse Experiments

Acetolactate synthase (ALS)–inhibiting herbicides are often used to control Italian ryegrass in winter wheat in Texas. An Italian ryegrass biotype near Waco, TX was evaluated for resistance to mesosulfuron in field and greenhouse experiments. Control of the biotype in the field was less than 10% with the label rate of mesosulfuron (15 g ai/ha). Greenhouse studies confirmed that the biotype was resistant to mesosulfuron; control of the biotype was less than 35% at 120 g ai/ha mesosulfuron. The herbicide dose required to reduce plant biomass of a susceptible and the Waco biotype by 50% (GR50) was 1.3 and 31 g ai/ha, respectively, indicating a resistance level of 24-fold in the Waco biotype. However, the Waco biotype was controlled with the acetyl-CoA carboxylase inhibitors diclofop and pinoxaden.

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Molecular basis of multiple resistance to herbicides inhibiting acetyl-CoA carboxylase and acetolactate synthase in American sloughgrass (Beckmannia syzigachne) from China

Crop and Pasture Science ◽

10.1071/cp16109 ◽

2016 ◽

Vol 67 (11) ◽

pp. 1208 ◽

Cited By ~ 1

Author(s):

Lang Pan ◽

Haitao Gao ◽

Han Wu ◽

Liyao Dong

Keyword(s):

Acetolactate Synthase ◽

Alternative Methods ◽

Multiple Resistance ◽

Homozygous Mutation ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Wheat Field ◽

Sequence Method ◽

Resistance To Herbicides ◽

Als Inhibitors

American sloughgrass (Beckmannia syzigachne Steud.) is a problematic grass that is widely distributed in wheat and oilseed rape fields in China. The herbicides fenoxaprop-P-ethyl and mesosulfuron-methyl failed to control B. syzigachne JCWJ-R populations collected from a wheat field in Jiangsu Province. Dose-response experiments showed that JCWJ-R was resistant to the acetyl-CoA carboxylase (ACCase) inhibitors fenoxaprop-P-ethyl (33.8-fold), haloxyfop-R-methyl (12.7-fold), clethodim (7.8-fold) and pinoxaden (11.6-fold), and to the acetolactate synthase (ALS) inhibitors mesosulfuron-methyl (15.9-fold), pyroxsulam (17.6-fold), flucarbazone-Na (10.7-fold) and imazethapyr (7-fold). Resistance to ALS inhibitors was due to a Pro-197-Ser mutation in the ALS gene and resistance to ACCase inhibitors was due to an Ile-1781-Leu mutation in the ACCase gene. A derived cleaved amplified polymorphic sequence method was developed to detect the ALS mutation in B. syzigachne. This was combined with a previously established method to detect Ile-1781-Leu, and the mutation frequency and homozygous mutation rates in the JCWJ-R population were determined. The evolution of multiple resistance to ACCase and ALS inhibitors in this B. syzigachne population indicated that alternative methods should be developed to control resistant weeds.

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Interactions Between Quizalofop-p-ethyl and Acetolactate Synthase–Inhibiting Herbicides in Acetyl-coA Carboxylase Inhibitor–Resistant Rice Production

Weed Technology ◽

10.1017/wet.2018.15 ◽

2018 ◽

Vol 32 (3) ◽

pp. 297-303 ◽

Cited By ~ 5

Author(s):

Samer Y. Rustom ◽

Eric P. Webster ◽

David C. Blouin ◽

Benjamin M. McKnight

Keyword(s):

Field Study ◽

Acetolactate Synthase ◽

Rice Production ◽

Research Station ◽

Acetyl Coa Carboxylase ◽

Red Rice ◽

Acetyl Coa

AbstractA field study was conducted in 2015 and 2016 at the H. Rouse Caffey Rice Research Station (RRS) to evaluate antagonistic, synergistic, or neutral interactions of quizalofop when mixed with ALS-inhibiting herbicides labeled in rice production. Quizalofop was applied at 120 g ai ha−1. Mixture herbicides included penoxsulam at 40 g ai ha−1, penoxsulam+triclopyr at 352 g ai ha−1, halosulfuron at 53 g ai ha−1, bispyribac at 34 g ai ha−1, orthosulfamuron+halosulfuron at 94 g ai ha−1, orthosulfamuron+quinclorac at 491 g ai ha−1, imazosulfuron at 211 g ai ha−1, and bensulfuron at 43 g ai ha−1. All ALS herbicides mixed with quizalofop indicated antagonistic responses for red rice, CL-111, CLXL 745, or barnyardgrass control at either 14 or 28 days after treatment (DAT). At 28 DAT, quizalofop mixed with penoxsulam or bispyribac controlled barnyardgrass 34 to 38%, compared with an expected control of 97%. In addition, these same mixtures controlled red rice, CL-111, and CLXL-745 61 to 67% at 28 DAT compared with an expected control of 96 to 97%. A second application of quizalofop at 120 g ha−1was applied at 28 DAT. At 42 DAT, neutral responses were indicated for all mixtures except with quizalofop mixed with penoxsulam containing products.

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