scholarly journals Transport of Salicylic Acid in Tobacco Necrosis Virus-Infected Cucumber Plants

1996 ◽  
Vol 112 (2) ◽  
pp. 787-792 ◽  
Author(s):  
W. Molders ◽  
A. Buchala ◽  
J. P. Metraux
Keyword(s):  
Salicylic Acid ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus

scholarly journals Salicylic Acid Biosynthetic Genes Expressed in Pseudomonas fluorescens Strain P3 Improve the Induction of Systemic Resistance in Tobacco Against Tobacco Necrosis Virus

1998 ◽  
Vol 88 (7) ◽  
pp. 678-684 ◽  
Author(s):  
M. Maurhofer ◽  
C. Reimmann ◽  
P. Schmidli-Sacherer ◽  
S. Heeb ◽  
D. Haas ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Systemic Acquired Resistance ◽  
Biocontrol Agent ◽  
Acquired Resistance ◽  
Expression Vectors ◽  
Systemic Resistance ◽  
Tobacco Necrosis Virus ◽  
Induce Systemic Resistance ◽  
Necrosis Virus

Application of salicylic acid induces systemic acquired resistance in tobacco. pchA and pchB, which encode for the biosynthesis of salicylic acid in Pseudomonas aeruginosa, were cloned into two expression vectors, and these constructs were introduced into two root-colonizing strains of P. fluorescens. Introduction of pchBA into strain P3, which does not produce salicylic acid, rendered this strain capable of salicylic acid production in vitro and significantly improved its ability to induce systemic resistance in tobacco against tobacco necrosis virus. Strain CHA0 is a well-described biocontrol agent that naturally produces salicylic acid under conditions of iron limitation. Introduction of pchBA into CHA0 increased the production of salicylic acid in vitro and in the rhizosphere of tobacco, but did not improve the ability of CHA0 to induce systemic resistance in tobacco. In addition, these genes did not improve significantly the capacity of strains P3 and CHA0 to suppress black root rot of tobacco in a gnotobiotic system.

scholarly journals Regioselective Base-induced Condensations of Acrylic Acid Derivatives

1999 ◽  
Vol 23 (3) ◽  
pp. 174-175
Author(s):  
E. Abdel-Ghani
Keyword(s):  
Carboxylic Acid ◽  
Acrylic Acid ◽  
Dicarboxylic Acid ◽  
Ethyl Acetoacetate ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus ◽  
Antiviral Activities

The orientation of cyclization of the reaction of methyl aroylacrylate (1) and aroylacrylic acid (8) with ethyl acetoacetate and/or thiourea leading to the formation of 4-aroylmethylcyclopentane-1,3-dione (2) 5-aryl-3-oxocyclohexene-1,2-dicarboxylic acid (9), 2-imino-5-aroylmethylthiazolidin-4-one (11) and 6-aryl-2-sulfonylpyrimidine-4-carboxylic acid (14) depends on the medium employed; some compounds show moderate antiviral activities against tobacco necrosis virus.

scholarly journals The 3' untranslated region of satellite tobacco necrosis virus RNA stimulates translation in vitro.

1993 ◽  
Vol 13 (6) ◽  
pp. 3340-3349 ◽  
Author(s):  
X Danthinne ◽  
J Seurinck ◽  
F Meulewaeter ◽  
M Van Montagu ◽  
M Cornelissen
Keyword(s):  
Tobacco Necrosis Virus ◽  
Translation System ◽  
Coding Region ◽  
Necrosis Virus ◽  
Untranslated Sequence ◽  
Virus Rna ◽  
Free Translation ◽  
Order Of Magnitude ◽  
A Cell

The RNA of satellite tobacco necrosis virus (STNV) is a monocistronic messenger that lacks both a 5' cap structure and a 3' poly(A) tail. We show that in a cell-free translation system derived from wheat germ, STNV RNA lacking the 600-nucleotide trailer is translated an order of magnitude less efficiently than full-size RNA. Deletion analyses positioned the translational enhancer domain (TED) within a conserved hairpin structure immediately downstream from the coat protein cistron. TED enhances translation when fused to a heterologous mRNA, but the level of enhancement depends on the nature of the 5' untranslated sequence and is maximal in combination with the STNV leader. The STNV leader and TED have two regions of complementarity. One of the complementary regions in TED resembles picornavirus box A, which is involved in cap-independent translation but which is located upstream of the coding region.

Translation of satellite tobacco necrosis virus ribonucleic acid. II. Initiation of in vitro translation in procaryotic and eucaryotic systems

Biochemistry ◽  
1972 ◽  
Vol 11 (11) ◽  
pp. 2014-2019 ◽  
Author(s):  
Ronald E. Lundquist ◽  
Jerome M. Lazar ◽  
William H. Klein ◽  
John M. Clark
Keyword(s):  
Ribonucleic Acid ◽  
In Vitro Translation ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus
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