scholarly journals The Small GTPase Rab5a Is Essential for Intracellular Transport of Proglutelin from the Golgi Apparatus to the Protein Storage Vacuole and Endosomal Membrane Organization in Developing Rice Endosperm

2011 ◽  
Vol 157 (2) ◽  
pp. 632-644 ◽  
Author(s):  
Masako Fukuda ◽  
Mio Satoh-Cruz ◽  
Liuying Wen ◽  
Andrew J. Crofts ◽  
Aya Sugino ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Intracellular Transport ◽  
Small Gtpase ◽  
Membrane Organization ◽  
Rice Endosperm ◽  
Protein Storage ◽  
Endosomal Membrane ◽  
Protein Storage Vacuole ◽  
Storage Vacuole

scholarly journals Vacuolar Storage Proteins Are Sorted in the Cis-Cisternae of the Pea Cotyledon Golgi Apparatus

2001 ◽  
Vol 152 (1) ◽  
pp. 41-50 ◽  
Author(s):  
Stefan Hillmer ◽  
Ali Movafeghi ◽  
David G. Robinson ◽  
Giselbert Hinz
Keyword(s):  
Golgi Apparatus ◽  
Storage Proteins ◽  
Spatial Separation ◽  
Golgi Stack ◽  
Protein Storage ◽  
Protein Storage Vacuole ◽  
Vacuolar Sorting ◽  
Sorting Receptor ◽  
Storage Vacuole ◽  
Dense Vesicles

Developing pea cotyledons contain functionally different vacuoles, a protein storage vacuole and a lytic vacuole. Lumenal as well as membrane proteins of the protein storage vacuole exit the Golgi apparatus in dense vesicles rather than in clathrin-coated vesicles (CCVs). Although the sorting receptor for vacuolar hydrolases BP-80 is present in CCVs, it is not detectable in dense vesicles. To localize these different vacuolar sorting events in the Golgi, we have compared the distribution of vacuolar storage proteins and of α-TIP, a membrane protein of the protein storage vacuole, with the distribution of the vacuolar sorting receptor BP-80 across the Golgi stack. Analysis of immunogold labeling from cryosections and from high pressure frozen samples has revealed a steep gradient in the distribution of the storage proteins within the Golgi stack. Intense labeling for storage proteins was registered for the cis-cisternae, contrasting with very low labeling for these antigens in the trans-cisternae. The distribution of BP-80 was the reverse, showing a peak in the trans-Golgi network with very low labeling of the cis-cisternae. These results indicate a spatial separation of different vacuolar sorting events in the Golgi apparatus of developing pea cotyledons.

scholarly journals Delivery of Prolamins to the Protein Storage Vacuole in Maize Aleurone Cells

2011 ◽  
Vol 23 (2) ◽  
pp. 769-784 ◽  
Author(s):  
Francisca C. Reyes ◽  
Taijoon Chung ◽  
David Holding ◽  
Rudolf Jung ◽  
Richard Vierstra ◽  
...  
Keyword(s):  
Protein Storage ◽  
Aleurone Cells ◽  
Protein Storage Vacuole ◽  
Storage Vacuole
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