scholarly journals Molecular and Biochemical Characterization of AtPAP15, a Purple Acid Phosphatase with Phytase Activity, in Arabidopsis

2009 ◽  
Vol 151 (1) ◽  
pp. 199-209 ◽  
Author(s):  
Ruibin Kuang ◽  
Kam-Ho Chan ◽  
Edward Yeung ◽  
Boon Leong Lim
Keyword(s):  
Acid Phosphatase ◽  
Biochemical Characterization ◽  
Phytase Activity ◽  
Purple Acid Phosphatase

Biochemical characterization of the tartrate-resistant acid phosphatase of human spleen with leukemic reticuloendotheliosis as a pyrophosphatase.

1977 ◽  
Vol 23 (1) ◽  
pp. 89-94 ◽  
Author(s):  
K W Lam ◽  
L T Yam
Keyword(s):  
Acid Phosphatase ◽  
Biochemical Characterization ◽  
Normal Animal ◽  
Tartrate Resistant Acid Phosphatase ◽  
Animal Tissues ◽  
Human Spleen ◽  
Optimal Activity ◽  
Catalytic Function ◽  
Hydrolysis Of

Abstract A tartrate-resistant acid phosphatase was isolated from a human leukemic spleen by freeze-thawing in saline and purified by repeated chromatography on carboxymethyl-cellulose. The purified enzyme has a molecular weight of 64 000. It catalyzes the hydrolysis of inorganic and organic pyrophosphate as well as the phenolic ester of monoorthophosphate, with optimal activity between pH 5 and 6. However, there is no activity toward mono-orthophosphate esters of aliphatic alcohols. The present data have identified its catalytic function as a pyrophosphatase. However, it has properties different from the pyrophosphatase previously observed in normal animal tissues.

scholarly journals Biochemical and Molecular Characterization of PvPAP3, a Novel Purple Acid Phosphatase Isolated from Common Bean Enhancing Extracellular ATP Utilization

2009 ◽  
Vol 152 (2) ◽  
pp. 854-865 ◽  
Author(s):  
Cuiyue Liang ◽  
Jiang Tian ◽  
Hon-Ming Lam ◽  
Boon Leong Lim ◽  
Xiaolong Yan ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Common Bean ◽  
Molecular Characterization ◽  
Extracellular Atp ◽  
Purple Acid Phosphatase

Phytase activity in tobacco (Nicotiana tabacum) root exudates is exhibited by a purple acid phosphatase

2008 ◽  
Vol 69 (2) ◽  
pp. 365-373 ◽  
Author(s):  
Shiu-Cheung Lung ◽  
Andy Leung ◽  
Rainbow Kuang ◽  
Yu Wang ◽  
Priscilla Leung ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Nicotiana Tabacum ◽  
Root Exudates ◽  
Phytase Activity ◽  
Purple Acid Phosphatase

The Demonstration of Lysosomes in Mouse Skin

1964 ◽  
Vol s3-105 (69) ◽  
pp. 73-78
Author(s):  
J. V. DIENGDOH
Keyword(s):  
Acid Phosphatase ◽  
Biochemical Characterization ◽  
Biochemical Study ◽  
Hydrolytic Enzymes ◽  
Mouse Skin ◽  
Freezing And Thawing ◽  
Sectioning Method ◽  
Repeated Freezing ◽  
Triton X

Lysosomes, as demonstrated biochemically in the liver, are subcellular particles containing a group of hydrolytic enzymes enclosed by a membrane-like barrier. They are apparently inactive in the normal state, but when subjected to various forms of injurious treatments the enzymes associated with them are released. The existence of lysosomes in skin is demonstrated in the present communication. The resistance of this tissue to homogenization makes the biochemical study of lysosomes very difficult. Yet, by the application of histochemical methods to sections of skin, it has been possible to use the same criteria as would be employed in the biochemical characterization of these organelles. By using the controlled-temperature freezing-sectioning method it has been possible to obtain frozen sections in which cytoplasmic particles could be demonstrated which were enzymically inactive for acid phosphatase until the sections were subjected to such injurious treatments as heat, repeated freezing and thawing, hypotonic solutions, distilled water, and ‘triton-X-100’. Since the subcellular particles demonstrated behaved in the same manner as lysosomes prepared biochemically from liver, it is concluded that the cytoplasmic organelles staining for acid phosphatase in mouse skin are lysosomes as biochemically defined.

Sign in / Sign up

Export Citation Format

Share Document