scholarly journals Gene Expression Profiling Reveals Defined Functions of the ATP-Binding Cassette Transporter COMATOSE Late in Phase II of Germination

2007 ◽  
Vol 143 (4) ◽  
pp. 1669-1679 ◽  
Author(s):  
Esther Carrera ◽  
Tara Holman ◽  
Anne Medhurst ◽  
Wendy Peer ◽  
Heike Schmuths ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Phase Ii ◽  
Expression Profiling ◽  
Atp Binding ◽  
Atp Binding Cassette Transporter ◽  
Atp Binding Cassette

scholarly journals Gene Expression Profiling of Transporters in the Solute Carrier and ATP-Binding Cassette Superfamilies in Human Eye Substructures

2013 ◽  
Vol 10 (2) ◽  
pp. 650-663 ◽  
Author(s):  
Amber Dahlin ◽  
Ethan Geier ◽  
Sophie L. Stocker ◽  
Cheryl D. Cropp ◽  
Elena Grigorenko ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Atp Binding ◽  
Human Eye ◽  
Solute Carrier ◽  
Atp Binding Cassette

scholarly journals Gene expression profiling identifies liver X receptor alpha as an estrogen-regulated gene in mouse adipose tissue

2004 ◽  
Vol 32 (3) ◽  
pp. 879-892 ◽  
Author(s):  
L Lundholm ◽  
S Moverare ◽  
KR Steffensen ◽  
M Nilsson ◽  
M Otsuki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Gene Expression Profiling ◽  
White Adipose Tissue ◽  
Expression Profiling ◽  
Liver X Receptor ◽  
Atp Binding ◽  
Mrna Levels ◽  
Ovariectomized Mice ◽  
Atp Binding Cassette

Estrogens reduce adipose tissue mass in both humans and animals. The molecular mechanisms for this effect are, however, not well characterized. We took a gene expression profiling approach to study the direct effects of estrogen on mouse white adipose tissue (WAT). Female ovariectomized mice were treated for 10, 24 and 48 h with 17beta-estradiol or vehicle. RNA was extracted from gonadal fat and hybridized to Affymetrix MG-U74Av2 arrays. 17beta-Estradiol was shown to decrease mRNA expression of liver X receptor (LXR) alpha after 10 h of treatment compared with the vehicle control. The expression of several LXRalpha target genes, such as sterol regulatory element-binding protein 1c, apolipoprotein E, phospholipid transfer protein, ATP-binding cassette A1 and ATP-binding cassette G1, was similarly decreased. We furthermore identified a 1.5 kb LXRalpha promoter fragment that is negatively regulated by estrogen. Several genes involved in lipogenesis and lipolysis were identified as novel targets that could mediate estrogenic effects on adipose tissue. Finally, we show that ERalpha is the main estrogen receptor expressed in mouse white adipose tissue (WAT) with mRNA levels several hundred times higher than those of ERbeta mRNA.

Statins downregulate ATP-binding-cassette transporter A1 gene expression in macrophages

2004 ◽  
Vol 316 (3) ◽  
pp. 790-794 ◽  
Author(s):  
Hirohito Sone ◽  
Hitoshi Shimano ◽  
Miao Shu ◽  
Masanori Nakakuki ◽  
Akimitsu Takahashi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Atp Binding ◽  
Atp Binding Cassette Transporter ◽  
Atp Binding Cassette

scholarly journals The Zinc Finger Protein 202 (ZNF202) Is a Transcriptional Repressor of ATP Binding Cassette Transporter A1 (ABCA1) and ABCG1 Gene Expression and a Modulator of Cellular Lipid Efflux

2001 ◽  
Vol 276 (15) ◽  
pp. 12427-12433 ◽  
Author(s):  
Mustafa Porsch-Özcürümez ◽  
Thomas Langmann ◽  
Susanne Heimerl ◽  
Hana Borsukova ◽  
Wolfgang E. Kaminski ◽  
...  
Keyword(s):  
Gene Expression ◽  
Zinc Finger ◽  
Transcriptional Repressor ◽  
Atp Binding ◽  
Raw264.7 Macrophages ◽  
Atp Binding Cassette Transporter ◽  
Lipid Efflux ◽  
Functional Linkage ◽  
Scan Domain ◽  
Atp Binding Cassette

The zinc finger gene 202 (ZNF202) located within a hypoalphalipoproteinemia susceptibility locus on chromosome 11q23 is a transcriptional repressor of various genes involved in lipid metabolism. To provide further evidence for a functional linkage between ZNF202 and hypoalphalipoproteinemia, we investigated the effect of ZNF202 expression on ATP binding cassette transporter A1 (ABCA1) and ABCG1. ABCA1 is a key regulator of the plasma high density lipoprotein pool size, whereas ABCG1 is another mediator of cellular cholesterol and phospholipid efflux in human macrophage. We demonstrate here that the full-length ZNF202m1 isoform binds to GnT repeats within the promotors of ABCA1 (−229/−210) and ABCG1 (−572/−552). ZNF202m1 expression in HepG2 cells dose-dependently repressed the promotor activities of ABCA1 and ABCG1. This transcriptional effect required the presence of the SCAN domain in ZNF202 and the functional integrity of a TATA box at position −24 of ABCA1, whereas the presence of GnT binding motifs was nonessential. The state of ZNF202 SCAN domain oligomerization affected the ability of the adjacent ZNF202 Krüppel-associated box domain to recruit the transcriptional corepressor KAP1. Overexpression of ZNF202m1 in RAW264.7 macrophages prevented the induction of ABCA1 gene expression by 20(S)OH-cholesterol and 9-cis-retinoic acid, further substantiating the interference of ZNF202 in critical elements of transcriptional activation. Finally, HDL and apoAImediated lipid efflux was significantly reduced in RAW264.7 cells stably expressing ZNF202m1. In conclusion, we have identified ABCA1 and ABCG1 as target genes for ZNF202-mediated repression and thus, provide evidence for a functional linkage between ZNF202 and hypoalphalipoproteinemia.

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