scholarly journals Functional Characterization of Ice Plant SKD1, an AAA-Type ATPase Associated with the Endoplasmic Reticulum-Golgi Network, and Its Role in Adaptation to Salt Stress

2006 ◽  
Vol 141 (1) ◽  
pp. 135-146 ◽  
Author(s):  
Yingtzy Jou ◽  
Chih-Pin Chiang ◽  
Guang-Yuh Jauh ◽  
Hungchen Emilie Yen
Keyword(s):  
Endoplasmic Reticulum ◽  
Salt Stress ◽  
Functional Characterization ◽  
Ice Plant ◽  
Golgi Network

Cloning and functional characterization of HKT1 and AKT1 genes of Fragaria spp.—Relationship to plant response to salt stress

2017 ◽  
Vol 210 ◽  
pp. 9-17 ◽  
Author(s):  
Miguel Garriga ◽  
Natalia Raddatz ◽  
Anne-Aliénor Véry ◽  
Hervé Sentenac ◽  
María E. Rubio-Meléndez ◽  
...  
Keyword(s):  
Salt Stress ◽  
Functional Characterization ◽  
Plant Response

Identification and functional characterization of a novel human protein highly related to the yeast dynamin-like GTPase Vps1p

1998 ◽  
Vol 111 (10) ◽  
pp. 1341-1349 ◽  
Author(s):  
M. Imoto ◽  
I. Tachibana ◽  
R. Urrutia
Keyword(s):  
Endoplasmic Reticulum ◽  
Cho Cells ◽  
Mammalian Cells ◽  
Functional Characterization ◽  
Luciferase Reporter ◽  
Pleckstrin Homology ◽  
Rich Region ◽  
Gtpase Domain ◽  
Proline Rich Region

Dynamin proteins containing a GTPase domain, a pleckstrin homology motif and a proline-rich tail participate in receptor-mediated endocytosis in organisms ranging from insects to vertebrates. In addition, dynamin-related GTPases, such as the yeast Golgi protein Vps1p, which lack both the pleckstrin homology motif and the proline-rich region, participate in vesicular transport within the secretory pathway in lower eukaryotes. However, no data is available on the existence of Vps1p-like proteins in mammalian cells. In this study, we report the identification and characterization of a novel gene encoding a human dynamin-related protein, DRP1, displaying high similarity to the Golgi dynamin-like protein Vps1p from yeast and to a Caenorhabditis elegans protein deposited in the databank. These proteins are highly conserved in their N-terminal tripartite GTPase domain but lack the pleckstrin homology motif and proline-rich region. Northern blot analysis reveals that the DRP1 mRNA is detected at high levels in human muscle, heart, kidney and brain. Immunolocalization studies in Chinese hamster ovary (CHO) cells using an epitope-tagged form of DRP1 and confocal microscopy show that this protein is concentrated in a perinuclear region that labels with the endoplasmic reticulum marker DiOC6(3) and the Golgi marker C5-DMB-Cer. In addition, the localization of DRP1 is highly similar to the localization of the endoplasmic reticulum and cis-Golgi GTPase Rab1A, but not to the staining for the trans-Golgi GTPase Rab6. Furthermore, overexpression of a cDNA encoding a GTP binding site mutant of DRP1 (DRP1(K38E)) in CHO cells decreases the amount of a secreted luciferase reporter protein, whereas the overexpression of wild-type DRP1 increases the secretion of this marker. Together, these results constitute the first structural and functional characterization of a mammalian protein similar to the yeast dynamin-related GTPase Vps1p and indicate that the participation of these proteins in secretion has been conserved throughout evolution.

scholarly journals Functional characterization of the Arabidopsis SERRATE under salt stress

2020 ◽  
Author(s):  
Minghui Mou ◽  
Qijuan Wang ◽  
Yanli Chen ◽  
Diqiu Yu ◽  
Ligang Chen
Keyword(s):  
Salt Stress ◽  
Functional Characterization

scholarly journals Functional Characterization of ERp18, a New Endoplasmic Reticulum-located Thioredoxin Superfamily Member

2003 ◽  
Vol 278 (31) ◽  
pp. 28912-28920 ◽  
Author(s):  
Heli I. Alanen ◽  
Richard A. Williamson ◽  
Mark J. Howard ◽  
Anna-Kaisa Lappi ◽  
Heli P. Jäntti ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Functional Characterization

Functional characterization of orchardgrass endoplasmic reticulum-resident Hsp90 (DgHsp90) as a chaperone and an ATPase

2009 ◽  
Vol 47 (10) ◽  
pp. 859-866 ◽  
Author(s):  
Joon-Yung Cha ◽  
Min Hee Jung ◽  
Netty Ermawati ◽  
Mukhamad Su'udi ◽  
Gyu-Jin Rho ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Functional Characterization

Isolation and functional characterization of salt-stress induced RCI2-like genes from Medicago sativa and Medicago truncatula

2015 ◽  
Vol 128 (4) ◽  
pp. 697-707 ◽  
Author(s):  
Ruicai Long ◽  
Fan Zhang ◽  
Zhenyi Li ◽  
Mingna Li ◽  
Lili Cong ◽  
...  
Keyword(s):  
Salt Stress ◽  
Medicago Sativa ◽  
Medicago Truncatula ◽  
Functional Characterization
Sign in / Sign up

Export Citation Format

Share Document