scholarly journals The DMI1 and DMI2 Early Symbiotic Genes of Medicago truncatula Are Required for a High-Affinity Nodulation Factor-Binding Site Associated to a Particulate Fraction of Roots

2005 ◽  
Vol 140 (1) ◽  
pp. 365-373 ◽  
Author(s):  
Bridget V. Hogg ◽  
Julie V. Cullimore ◽  
Raoul Ranjeva ◽  
Jean-Jacques Bono
2002 ◽  
Vol 15 (8) ◽  
pp. 834-839 ◽  
Author(s):  
Frédéric Gressent ◽  
Natacha Mantegazza ◽  
Julie V. Cullimore ◽  
Hugues Driguez ◽  
Raoul Ranjeva ◽  
...  

The lipo-chitooligosaccharidic Nod factors produced by rhizobia are key molecules in the establishment of symbiosis with legumes and probably are recognized by the host plant via specific receptors. Here, we report on the presence of a binding site in cell cultures of Phaseolus vulgaris displaying a high affinity for Nod factors from Rhizobium tropici (NodRt-V) (Me, S, C18:1), a symbiont of this legume. The binding site shares common properties with NFBS2, a Nod-factor binding site previously characterised in Medicago varia, in terms of affinity, preferential plasma-membrane location, and sensitivity to proteases and lysine reactive reagents. However, the bean site poorly recognizes the Nod factors produced by Sinorhizobium meliloti, the symbiont of Medicago. The study of selectivity toward the Nod factors reveals that the length and degree of unsaturation of the acyl chain and the length of the oligosaccharidic moiety are important determinants of high affinity binding to the bean site; whereas, the N-methyl and O-sulfuryl groups play a minor role. Thus, the common characteristics of P. vulgaris and M. varia Nod-factor binding sites suggest that they probably correspond to structurally related proteins, but their different selectivity suggests that they may be involved in a differential perception system for Nod factors in legumes.


FEBS Journal ◽  
2009 ◽  
Vol 277 (2) ◽  
pp. 413-427 ◽  
Author(s):  
Eric Calvo ◽  
Fuyuki Tokumasu ◽  
Daniella M. Mizurini ◽  
Peter McPhie ◽  
David L. Narum ◽  
...  

2008 ◽  
Vol 283 (30) ◽  
pp. 21267-21277 ◽  
Author(s):  
Cristina Basilico ◽  
Addolorata Arnesano ◽  
Maria Galluzzo ◽  
Paolo M. Comoglio ◽  
Paolo Michieli

Cell Reports ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. 1937-1946 ◽  
Author(s):  
Abraham P. Fong ◽  
Zizhen Yao ◽  
Jun Wen Zhong ◽  
Nathan M. Johnson ◽  
Gist H. Farr ◽  
...  

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