scholarly journals Expression of Erwinia uredovora Phytoene Desaturase in Synechococcus PCC7942 Leading to Resistance against a Bleaching Herbicide

1994 ◽  
Vol 104 (1) ◽  
pp. 119-125 ◽  
Author(s):  
U. Windhovel ◽  
B. Geiges ◽  
G. Sandmann ◽  
P. Boger
Keyword(s):  
Phytoene Desaturase ◽  
Synechococcus Pcc7942 ◽  
Bleaching Herbicide

Bleaching Herbicide Norflurazon Inhibits Phytoene Desaturase by Competition with the Cofactors

2001 ◽  
Vol 49 (11) ◽  
pp. 5270-5272 ◽  
Author(s):  
Jürgen Breitenbach ◽  
Changfu Zhu ◽  
Gerhard Sandmann
Keyword(s):  
Phytoene Desaturase ◽  
Bleaching Herbicide

scholarly journals Bleaching Herbicide Flurtamone Interferes with Phytoene Desaturase

1990 ◽  
Vol 94 (2) ◽  
pp. 476-478 ◽  
Author(s):  
Gerhard Sandmann ◽  
Carl E. Ward ◽  
William C. Lo ◽  
Jon O. Nagy ◽  
Peter Böger
Keyword(s):  
Phytoene Desaturase ◽  
Bleaching Herbicide

Cloning a Gene Coding for Norflurazon Resistance in Cyanobacteria

1990 ◽  
Vol 45 (5) ◽  
pp. 482-486 ◽  
Author(s):  
Daniel Chamovitz ◽  
Iris Pecker ◽  
Gerhard Sandmann ◽  
Peter Böger ◽  
Joseph Hirschberg
Keyword(s):  
Herbicide Resistance ◽  
Phytoene Desaturase ◽  
Wild Type ◽  
Photosynthetic Organisms ◽  
Gene Coding ◽  
Herbicide Resistance Gene ◽  
Phytoene Dehydrogenase ◽  
Synechococcus Pcc7942 ◽  
Resistance Trait ◽  
Resistant Mutants

The herbicide norflurazon inhibits carotene biosynthesis in photosynthetic organisms by blocking the enzyme phytoene dehydrogenase (= phytoene desaturase). We have isolated norflurazon-resistant mutants of the cyanobacterium Synechococcus PCC7942. The herbicide- resistance gene from the mutant NFZ4 has been cloned by genetic complementation of the resistance trait in wild type cells. The experiment described here illustrates the usefulness of employing cyanobacteria to clone herbicide-resistance genes in a quick and simple way.

scholarly journals Mutation of the PHYTOENE DESATURASE 3 gene causes yellowish-white petals in Brassica napus

2021 ◽  
Author(s):  
Chuanji Zhao ◽  
Luqman Bin Safdar ◽  
Meili Xie ◽  
Meijuan Shi ◽  
Zhixue Dong ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Phytoene Desaturase ◽  
Yellowish White

scholarly journals Expression in Escherichia coli, purification, and reactivation of the recombinant Erwinia uredovora phytoene desaturase.

1992 ◽  
Vol 267 (28) ◽  
pp. 19891-19895
Author(s):  
P.D. Fraser ◽  
N Misawa ◽  
H Linden ◽  
S Yamano ◽  
K Kobayashi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Phytoene Desaturase ◽  
Expression In Escherichia Coli

Phytoene desaturase-silenced citrus as a trap crop with multiple cues to attract Diaphorina citri, the vector of Huanglongbing

Plant Science ◽  
2021 ◽  
Vol 308 ◽  
pp. 110930
Author(s):  
Nabil Killiny ◽  
Yasser Nehela ◽  
Justin George ◽  
Mahnaz Rashidi ◽  
Lukasz L. Stelinski ◽  
...  
Keyword(s):  
Phytoene Desaturase ◽  
Diaphorina Citri ◽  
Trap Crop ◽  
Multiple Cues

Optimisation of tobacco rattle virus-induced gene silencing in Arabidopsis

2006 ◽  
Vol 33 (4) ◽  
pp. 347 ◽  
Author(s):  
Changchun Wang ◽  
Xinzhong Cai ◽  
Xuemin Wang ◽  
Zhong Zheng
Keyword(s):  
Gene Silencing ◽  
High Throughput ◽  
Function Analysis ◽  
Tobacco Rattle Virus ◽  
Phytoene Desaturase ◽  
Growth Stages ◽  
Virus Induced Gene Silencing ◽  
Gene Functions ◽  
Genes Encoding ◽  
Gene Function Analysis

Arabidopsis thaliana (L.) Heynh. is a model plant species in which to study plant gene functions. Recently developed virus-induced gene silencing (VIGS) offers a rapid and high-throughput technique platform for gene function analysis. In this paper we report optimisation of tobacco rattle virus (TRV)-induced gene silencing in Arabidopsis. The parameters potentially affecting the efficiency of VIGS in Arabidopsis were investigated. These included the concentration and pre-incubation of Agrobacterium inocula (agro-inocula), the concentration of acetosyringone included in agro-inocula, the Agrobacterium inoculation (agro-inoculation) method, the ecotypes and the growth stages of Arabidopsis plants for agro-inoculation, and the growth temperature of agro-inoculated plants. The optimised VIGS procedure involves preparing the agro-inocula with OD600 of 2.0, pre-incubating for 2 h in infiltration buffer containing 200 μm acetosyringone, agro-inoculating by vacuum infiltration, and growth of agro-inoculated plants at 22 −24°C. Following this procedure consistent and highly efficient VIGS was achieved for the genes encoding phytoene desaturase (PDS) and actin in Arabidopsis. The silencing phenotype lasts for at least 6 weeks, and is applicable in at least seven ecotypes, including Col-0, Cvi-0, Sd, Nd-1, Ws-0, Bay-0 and Ler. TRV-induced VIGS was expressed not only in leaves, but also in stems, inflorescences and siliques. However, VIGS was not transmissible through seed to the subsequent generation. The optimised procedure of the TRV-induced gene silencing should facilitate high-throughput functional analysis of genes in Arabidopsis.

Mutations in Phytoene Desaturase Gene in Fluridone-Resistant Hydrilla (Hydrilla verticillata) Biotypes in Florida

Weed Science ◽  
2007 ◽  
Vol 55 (5) ◽  
pp. 412-420 ◽  
Author(s):  
Atul Puri ◽  
Gregory E. MacDonald ◽  
Fredy Altpeter ◽  
William T. Haller
Keyword(s):  
Herbicide Resistance ◽  
Single Point ◽  
Hydrilla Verticillata ◽  
The United States ◽  
Phytoene Desaturase ◽  
Day Length ◽  
Single Point Mutation ◽  
Aquatic Weed ◽  
Systemic Control

Hydrilla is one of the most serious aquatic weed problems in the United States, and fluridone is the only U.S. Environment Protection Agency (USEPA)–approved herbicide that provides relatively long-term systemic control. Recently, hydrilla biotypes with varying levels of fluridone resistance have been documented in Florida. One susceptible and five fluridone-resistant biotypes of hydrilla varying in resistance levels were maintained in 950-L tanks under ambient sunlight and day-length conditions from September 2004 to September 2005 in absence of fluridone. Because fluridone is an inhibitor of the enzyme phytoene desaturase (PDS), the gene for PDS (pds) was cloned from fluridone-susceptible and -resistant hydrilla biotypes. Somatic mutations in amino acid 304 of hydrilla PDS are known to confer herbicide resistance. We determinedpdssequence from these hydrilla biotypes at planting and 12-mo after planting. Two independent mutations at the arginine 304 codon ofpdswere found in the resistant hydrilla plants. The codon usage for arginine 304 is CGT, and a single point mutation yielding either serine (AGT) or histidine (CAT) was identified in different resistant hydrilla biotypes. There were no differences at codon 304 in the PDS protein of any hydrilla biotype 12-mo after planting. Several other mutations were also found in resistantpdsalleles, though their possible role in herbicide resistance is unclear.

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