scholarly journals Selected Components of the Shade-Avoidance Syndrome Are Displayed in a Normal Manner in Mutants of Arabidopsis thaliana and Brassica rapa Deficient in Phytochrome B

1993 ◽  
Vol 102 (4) ◽  
pp. 1179-1184 ◽  
Author(s):  
PRH. Robson ◽  
G. C. Whitelam ◽  
H. Smith
Keyword(s):  
Arabidopsis Thaliana ◽  
Brassica Rapa ◽  
Shade Avoidance ◽  
Phytochrome B ◽  
Shade Avoidance Syndrome ◽  
Normal Manner

scholarly journals Analysis of the Function of the Photoreceptors Phytochrome B and Phytochrome D in Nicotiana plumbaginifolia and Arabidopsis thaliana

2005 ◽  
Vol 46 (5) ◽  
pp. 790-796 ◽  
Author(s):  
Aurora Piñas Fernández ◽  
Patricia Gil ◽  
Ildiko Valkai ◽  
Ferenc Nagy ◽  
Eberhard Schäfer
Keyword(s):  
Arabidopsis Thaliana ◽  
Nicotiana Plumbaginifolia ◽  
Phytochrome B

scholarly journals Light Gradient-Based Screening of Arabidopsis thaliana on a 384-Well Type Plant Array Chip

Micromachines ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 191
Author(s):  
Youn-Hee Park ◽  
Je-Kyun Park
Keyword(s):  
Arabidopsis Thaliana ◽  
White Light ◽  
Solid Medium ◽  
Red Light ◽  
Phytochrome B ◽  
Wild Type ◽  
Light Gradient ◽  
Gradient Based ◽  
Light Effects ◽  
Germination And Growth

Arabidopsis thaliana (Arabidopsis), as a model for plant research, is widely used for various aspects of plant science. To provide a more sophisticated and microscopic environment for the germination and growth of Arabidopsis, we report a 384-well type plant array chip in which each Arabidopsis seed is independently seeded in a solid medium. The plant array chip is made of a poly(methyl methacrylate) (PMMA) acrylic material and is assembled with a home-made light gradient module to investigate the light effects that significantly affect the germination and growth of Arabidopsis. The light gradient module was used to observe the growth pattern of seedlings according to the intensity of the white light and to efficiently screen for the influence of the white light. To investigate the response to red light (600 nm), which stimulates seed germination, the light gradient module was also applied to the germination test. As a result, the germination results showed that the plant array chip can be used to simultaneously screen wild type seeds and phytochrome B mutant seeds on a single array chip according to the eight red light intensities.

scholarly journals Arabidopsis chloroplast J protein DJC75/CRRJ mediates nitrate-promoted seed germination in the dark

2020 ◽  
Vol 125 (7) ◽  
pp. 1091-1099
Author(s):  
Huai-Syuan Ciou ◽  
Yu-Lun Tsai ◽  
Chi-Chou Chiu
Keyword(s):  
Arabidopsis Thaliana ◽  
Seed Germination ◽  
Germination Rate ◽  
Red Light ◽  
Phytochrome B ◽  
Biosynthetic Gene ◽  
Unknown Mechanism ◽  
J Domain ◽  
Domain Protein ◽  
J Protein

Abstract Background and Aims Nitrate can stimulate seed germination of many plant species in the absence of light; however, the molecular mechanism of nitrate-promoted seed germination in the dark remains largely unclear and no component of this pathway has been identified yet. Here, we show that a plastid J-domain protein, DJC75/CRRJ, in arabidopsis (Arabidopsis thaliana) is important for nitrate-promoted seed germination in the dark. Methods The expression of DJC75 during imbibition in the dark was investigated. The seed germination rate of mutants defective in DJC75 was determined in the presence of nitrate when light cues for seed germination were eliminated by the treatment of imbibed seeds with a pulse of far-red light to inactivate phytochrome B (phyB), or by assaying germination in the dark with seeds harbouring the phyB mutation. The germination rates of mutants defective in CRRL, a J-like protein related to DJC75, and in two chloroplast Hsp70s were also measured in the presence of nitrate in darkness. Key Results DJC75 was expressed during seed imbibition in the absence of light. Mutants defective in DJC75 showed seed germination defects in the presence of nitrate when light cues for seed germination were eliminated. Mutants defective in CRRL and in two chloroplast Hsp70s also exhibited similar seed germination defects. Upregulation of gibberellin biosynthetic gene GA3ox1 expression by nitrate in imbibed phyB mutant seeds was diminished when DJC75 was knocked out. Conclusions Our data suggest that plastid J-domain protein DJC75 regulates nitrate-promoted seed germination in the dark by upregulation of expression of the gibberellin biosynthetic gene GA3ox1 through an unknown mechanism and that DJC75 may work in concert with chloroplast Hsp70s to regulate nitrate-promoted seed germination. DJC75 is the first pathway component identified for nitrate-promoted seed germination in the dark.

scholarly journals The Different Growth Responses of the Arabidopsis thaliana Leaf Blade and the Petiole during Shade Avoidance are Regulated by Photoreceptors and Sugar

2005 ◽  
Vol 46 (1) ◽  
pp. 213-223 ◽  
Author(s):  
Toshiaki Kozuka ◽  
Gorou Horiguchi ◽  
Gyung-Tae Kim ◽  
Maki Ohgishi ◽  
Tatsuya Sakai ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Leaf Blade ◽  
Shade Avoidance ◽  
Growth Responses
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