Double isotope-ratio thermometers: The influence of emission time scales

1999 ◽  
Vol 59 (5) ◽  
pp. 2660-2669 ◽  
Author(s):  
V. E. Viola ◽  
K. Kwiatkowski ◽  
W. A. Friedman
1989 ◽  
Vol 341 ◽  
pp. 293 ◽  
Author(s):  
David K. Taylor ◽  
R. L. Dickman

1998 ◽  
Vol 416 (1-2) ◽  
pp. 56-61 ◽  
Author(s):  
G.J Kunde ◽  
S Gaff ◽  
C.K Gelbke ◽  
T Glasmacher ◽  
M.J Huang ◽  
...  
Keyword(s):  

1978 ◽  
Vol 176 (3) ◽  
pp. 919-926 ◽  
Author(s):  
R J Burgess ◽  
J H Walker ◽  
R J Mayer

1. The double-isotope concept [Arias, Doyle & Schimke (1969) J. Biol. Chem. 224, 3303–3315] for the measurement of protein turnover was used to estimate the turnover of proteins in subcellular and submitochondrial fractions prepared from rat liver. 2. Double-isotope experiments with [3H]leucine as first precursor and [14C]leucine as second precursor were used to measure the turnover rates of proteins in subcellular and submitochondrial fractions. Solvent extraction procedures designed to remove lipids and nucleic acids from trichloroacetic acid precipitates only changed the isotope ratio of the microsomal fraction. It was not possible to measure turnover of proteins in mitochondrial and submitochondrial fractions with these precursors. 3. Double-isotope experiments were designed to minimize first-precursor reutilization by employing NaH14CO3. [3H]Arginine was used as second precursor. The turnover rates of protein in subcellular and submitochondrial fractions was measured. Solvent extraction procedures designed to remove lipids and nucleic acids showed changes in the isotope ratio for all subcellular fractions, especially in microsomal and detergent-soluble mitochondrial fractions. Isotope ratios of precipitates after solvent extraction indicate that, whereas considerable heterogeneity exists for the average rates of protein turnover in subcellular fractions, little heterogeneity is observed in the average rates of protein turnover in submitochondrial fractions.


1997 ◽  
Vol 620 (2) ◽  
pp. 214-228 ◽  
Author(s):  
Zhi Yong He ◽  
Li Min Duan ◽  
Gen Ming Jin ◽  
Zu Yu Li ◽  
He Yu Wu ◽  
...  

1993 ◽  
Vol 70 (23) ◽  
pp. 3534-3537 ◽  
Author(s):  
D. R. Bowman ◽  
G. F. Peaslee ◽  
N. Carlin ◽  
R. T. de Souza ◽  
C. K. Gelbke ◽  
...  
Keyword(s):  

2000 ◽  
Vol 8 (3) ◽  
pp. 355-360 ◽  
Author(s):  
P. M. Milazzo ◽  
G. Vannini ◽  
M. Bruno ◽  
N. Colonna ◽  
M. D’Agostino ◽  
...  

2020 ◽  
Vol 497 (4) ◽  
pp. 5076-5089
Author(s):  
Daniel T Haydon ◽  
Yusuke Fujimoto ◽  
Mélanie Chevance ◽  
J M Diederik Kruijssen ◽  
Mark R Krumholz ◽  
...  

ABSTRACT Recent observational studies aiming to quantify the molecular cloud lifecycle require the use of known ‘reference time-scales’ to turn the relative durations of different phases of the star formation process into absolute time-scales. We previously constrained the characteristic emission time-scales of different star formation rate (SFR) tracers, as a function of the SFR surface density and metallicity. However, we omitted the effects of dust extinction. Here, we extend our suite of SFR tracer emission time-scales by accounting for extinction, using synthetic emission maps of a high-resolution hydrodynamical simulation of an isolated, Milky Way-like disc galaxy. The stellar feedback included in the simulation is inefficient compared to observations, implying that it represents a limiting case in which the duration of embedded star formation (and the corresponding effect of extinction) is overestimated. Across our experiments, we find that extinction mostly decreases the SFR tracer emission time-scale, changing the time-scales by factors of 0.04–1.74, depending on the gas column density. UV filters are more strongly affected than H α filters. We provide the limiting correction factors as a function of the gas column density and flux sensitivity limit for a wide variety of SFR tracers. Applying these factors to observational characterizations of the molecular cloud lifecycle produces changes that broadly fall within the quoted uncertainties, except at high kpc-scale gas surface densities ($\Sigma _{\rm g}\gtrsim 20~{\mathrm{M_{\odot }\, pc^{-2}}}$). Under those conditions, correcting for extinction may decrease the measured molecular cloud lifetimes and feedback time-scales, which further strengthens previous conclusions that molecular clouds live for a dynamical time and are dispersed by early, pre-supernova feedback.


1998 ◽  
Vol 57 (4) ◽  
pp. 1824-1831 ◽  
Author(s):  
Zhi Yong He ◽  
Gen Ming Jin ◽  
Zu Yu Li ◽  
Li Min Duan ◽  
Guang Xi Dai ◽  
...  

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