Nuclear temperature measurements with the double isotope ratio technique: Influence of the experimental conditions

2000 ◽  
Vol 8 (3) ◽  
pp. 355-360 ◽  
Author(s):  
P. M. Milazzo ◽  
G. Vannini ◽  
M. Bruno ◽  
N. Colonna ◽  
M. D’Agostino ◽  
...  
1989 ◽  
Vol 341 ◽  
pp. 293 ◽  
Author(s):  
David K. Taylor ◽  
R. L. Dickman

1999 ◽  
Vol 59 (5) ◽  
pp. 2660-2669 ◽  
Author(s):  
V. E. Viola ◽  
K. Kwiatkowski ◽  
W. A. Friedman

1998 ◽  
Vol 630 (1-2) ◽  
pp. 160-167
Author(s):  
H. Xi ◽  
M.J. Huang ◽  
W.G. Lynch ◽  
M.B. Tsang ◽  
J.D. Dinius ◽  
...  

1998 ◽  
Vol 416 (1-2) ◽  
pp. 56-61 ◽  
Author(s):  
G.J Kunde ◽  
S Gaff ◽  
C.K Gelbke ◽  
T Glasmacher ◽  
M.J Huang ◽  
...  
Keyword(s):  

1986 ◽  
Vol 182 (2) ◽  
pp. 155-158 ◽  
Author(s):  
H.M. Xu ◽  
D.J. Fields ◽  
W.G. Lynch ◽  
M.B. Tsang ◽  
C.K. Gelbke ◽  
...  

1978 ◽  
Vol 176 (3) ◽  
pp. 919-926 ◽  
Author(s):  
R J Burgess ◽  
J H Walker ◽  
R J Mayer

1. The double-isotope concept [Arias, Doyle & Schimke (1969) J. Biol. Chem. 224, 3303–3315] for the measurement of protein turnover was used to estimate the turnover of proteins in subcellular and submitochondrial fractions prepared from rat liver. 2. Double-isotope experiments with [3H]leucine as first precursor and [14C]leucine as second precursor were used to measure the turnover rates of proteins in subcellular and submitochondrial fractions. Solvent extraction procedures designed to remove lipids and nucleic acids from trichloroacetic acid precipitates only changed the isotope ratio of the microsomal fraction. It was not possible to measure turnover of proteins in mitochondrial and submitochondrial fractions with these precursors. 3. Double-isotope experiments were designed to minimize first-precursor reutilization by employing NaH14CO3. [3H]Arginine was used as second precursor. The turnover rates of protein in subcellular and submitochondrial fractions was measured. Solvent extraction procedures designed to remove lipids and nucleic acids showed changes in the isotope ratio for all subcellular fractions, especially in microsomal and detergent-soluble mitochondrial fractions. Isotope ratios of precipitates after solvent extraction indicate that, whereas considerable heterogeneity exists for the average rates of protein turnover in subcellular fractions, little heterogeneity is observed in the average rates of protein turnover in submitochondrial fractions.


1999 ◽  
Vol 59 (3) ◽  
pp. 1567-1573 ◽  
Author(s):  
H. Xi ◽  
W. G. Lynch ◽  
M. B. Tsang ◽  
W. A. Friedman ◽  
D. Durand

1986 ◽  
Vol 34 (3) ◽  
pp. 850-857 ◽  
Author(s):  
C. Bloch ◽  
W. Benenson ◽  
E. Kashy ◽  
D. J. Morrissey ◽  
R. A. Blue ◽  
...  

1998 ◽  
Vol 431 (1-2) ◽  
pp. 8-14 ◽  
Author(s):  
H. Xi ◽  
M.B. Tsang ◽  
M.J. Huang ◽  
W.G. Lynch ◽  
J.D. Dinius ◽  
...  

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