In this paper, the role of milling cutter eccentricity, commonly referred to as runout, is explored to determine its effects on surface topography and milling forces. This work is motivated by the observation that commercially-available cutter bodies often exhibit variation in the teeth/insert radial locations as a result of manufacturing issues. Consequently, the chip load on individual cutting teeth varies periodically, which can lead to premature failure of the cutting edges. Additionally, this chip load variation increases the roughness of machined surfaces. This research isolates the effect of runout on cutting forces and the machined surface finish in a series of experiments completed on a precision milling machine with 0.1 μm positioning repeatability and 0.02 μm spindle error motion. The runout is varied in a controlled fashion and results compared between experiment and a comprehensive time-domain simulation.