Temperature-Sensitive Mutants of B. subtilis Defective in DNA Synthesis

1968 ◽  
Vol 33 (0) ◽  
pp. 307-312 ◽  
Author(s):  
J. D. Gross ◽  
D. Karamata ◽  
P. G. Hempstead
Keyword(s):  
Dna Synthesis ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants

Induced cellular DNA synthesis by ‘early’ and ‘late’ temperature-sensitive mutants of polyoma virus

1971 ◽  
Vol 177 (1046) ◽  
pp. 59-63 ◽  
Keyword(s):  
Dna Synthesis ◽  
Polyoma Virus ◽  
Viral Gene ◽  
Temperature Sensitive ◽  
Gene Products ◽  
Virus Growth ◽  
Temperature Sensitive Mutants ◽  
Cellular Dna

Temperature-sensitive mutants of polyoma virus have been examined to determine whether they are able to induce the synthesis of cellular DNA under conditions where viral gene products are defective. Two ‘early’ mutants, and one ‘late’ mutant of polyoma induce cellular DNA synthesis normally under conditions where virus growth is inhibited because viral gene products are defective.

Mutational blockage of DNA synthesis in Paramecium tetraurelia

1976 ◽  
Vol 54 (12) ◽  
pp. 2089-2097 ◽  
Author(s):  
E. L. Peterson ◽  
J. D. Berger
Keyword(s):  
Dna Synthesis ◽  
Deoxyribonucleic Acid ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Paramecium Tetraurelia ◽  
Selection System ◽  
Replication Process ◽  
Temperature Sensitive Mutants ◽  
Fold Enrichment ◽  
Comparison Of Experiments

One hundred and ninety-eight temperature-sensitive mutants of Paramecium tetraurelia were isolated after nitrosoguanidine mutagenesis. In some experiments, mutants were recovered with the aid of a bromouracil (BU) selection system. Fifty-six mutants showed cessation of cell division within one cell cycle at the restrictive temperature and were designated ts-0. Fourteen of the ts-0's showed a greater than 90% reduction in rnacronuclear deoxyribonucleic acid (DNA) synthesis at the restrictive temperature. Two ts-0. DNA-defective lines continued protein synthesis at greater than 50% the normal rate after arrest of DNA synthesis. Hence, these two mutants may be directly affected in the replication process itself. The two mutants are allelic and, in addition, a third 'leaky' allele was recovered. Comparison of experiments in which either BU selection or no selection was employed shows that a greater than 10-fold enrichment for ts mutants resulted from BU selection.

scholarly journals INHIBITION OF DNA SYNTHESIS AND INDUCTION OF LAMBDA PHAGE IN TEMPERATURE SENSITIVE MUTANTS OF ESCHERICHIA COLI

1966 ◽  
Vol 41 (3) ◽  
pp. 247-249 ◽  
Author(s):  
YOSHINOBU SUGINO ◽  
AKIKO SORAI ◽  
TOKIO KOGOMA ◽  
YUKINORI HIROTA ◽  
REIJI OKAZAKI
Keyword(s):  
Escherichia Coli ◽  
Dna Synthesis ◽  
Temperature Sensitive ◽  
Lambda Phage ◽  
Temperature Sensitive Mutants ◽  
Inhibition Of Dna Synthesis
Sign in / Sign up

Export Citation Format

Share Document