Protoplast wash solution for Agrobacterium transformation (APW)

2009 ◽  
Vol 2009 (2) ◽  
pp. pdb.rec11655-pdb.rec11655
Keyword(s):  
Agrobacterium Transformation ◽  
Wash Solution

scholarly journals Advances in Agrobacterium transformation and vector design result in high frequency targeted gene insertion in maize

2021 ◽  
Author(s):  
Dave Peterson ◽  
Pierluigi Barone ◽  
Brian Lenderts ◽  
Chris Schwartz ◽  
Lanie Feigenbutz ◽  
...  
Keyword(s):  
High Frequency ◽  
Agrobacterium Transformation ◽  
Gene Insertion ◽  
Design Result

Development of an Algorithm for Feed-Forward Chlorine Dosing of Lettuce Wash Operations and Correlation of Chlorine Profile with Escherichia coli O157:H7 Inactivation

2014 ◽  
Vol 77 (4) ◽  
pp. 558-566 ◽  
Author(s):  
BIN ZHOU ◽  
YAGUANG LUO ◽  
XIANGWU NOU ◽  
PATRICIA MILLNER
Keyword(s):  
Escherichia Coli ◽  
Reduction Potential ◽  
Free Chlorine ◽  
Organic Load ◽  
Escherichia Coli O157 ◽  
Feed Forward ◽  
E Coli ◽  
Wash Solution ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential

The dynamic interactions of chlorine and organic matter during a simulated fresh-cut produce wash process and the consequences for Escherichia coli O157:H7 inactivation were investigated. An algorithm for a chlorine feed-forward dosing scheme to maintain a stable chlorine level was further developed and validated. Organic loads with chemical oxygen demand of 300 to 800 mg/liter were modeled using iceberg lettuce. Sodium hypochlorite (NaOCl) was added to the simulated wash solution incrementally. The solution pH, free and total chlorine, and oxidation-reduction potential were monitored, and chlorination breakpoint and chloramine humps determined. The results indicated that the E. coli O157:H7 inactivation curve mirrored that of the free chlorine during the chlorine replenishment process: a slight reduction in E. coli O157:H7 was observed as the combined chlorine hump was approached, while the E. coli O157:H7 cell populations declined sharply after chlorination passed the chlorine hump and decreased to below the detection limit (<0.75 most probable number per ml) after the chlorination breakpoint was reached. While the amounts of NaOCl required for reaching the chloramine humps and chlorination breakpoints depended on the organic loads, there was a linear correlation between NaOCl input and free chlorine in the wash solution once NaOCl dosing passed the chlorination breakpoint, regardless of organic load. The data obtained were further exploited to develop a NaOCl dosing algorithm for maintaining a stable chlorine concentration in the presence of an increasing organic load. The validation tests results indicated that free chlorine could be maintained at target levels using such an algorithm, while the pH and oxidation-reduction potential were also stably maintained using this system.

Susceptibility of Maize Mesocotyl Culture to Agrobacterium Transformation and Its in vitro Regeneration

2018 ◽  
Vol 54 (8) ◽  
pp. 808-815
Author(s):  
V. N. Ovchinnikova ◽  
V. S. Sotchenko ◽  
Yu. V. Sotchenko ◽  
N. V. Varlamova ◽  
M. A. Rodionova ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Agrobacterium Transformation

Efficacy of an intimate hygiene wash solution on the global symptoms of acute vulvovaginitis in menopause women

Maturitas ◽  
2015 ◽  
Vol 81 (1) ◽  
pp. 180
Author(s):  
Luis Sanfrutos ◽  
Raquel Oliva ◽  
Paloma Pino ◽  
Miguel Rejas Rejas ◽  
Antonio Navarro ◽  
...  
Keyword(s):  
Wash Solution

Wash solution 1

2007 ◽  
Vol 2007 (23) ◽  
pp. pdb.rec10910-pdb.rec10910
Keyword(s):  
Wash Solution

10X Drosophila embryo wash solution

2007 ◽  
Vol 2007 (17) ◽  
pp. pdb.rec11114-pdb.rec11114
Keyword(s):  
Drosophila Embryo ◽  
Wash Solution
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