10X Drosophila embryo wash solution

2007 ◽  
Vol 2007 (17) ◽  
pp. pdb.rec11114-pdb.rec11114
Keyword(s):  
Drosophila Embryo ◽  
Wash Solution

Laser scanning confocal microscopic analysis of cytoskeletal and nuclear reorganization in live Drosophila embryos

1993 ◽  
Vol 51 ◽  
pp. 174-175
Author(s):  
William Theurkauf
Keyword(s):  
Laser Scanning ◽  
Microscopic Analysis ◽  
Large Cell ◽  
Early Embryo ◽  
Drosophila Embryo ◽  
Cortical Actin ◽  
Nuclear Reorganization ◽  
Cytoskeletal Elements ◽  
Microtubule Structure ◽  
Drosophila Embryos

Cell division in eucaryotes depends on coordinated changes in nuclear and cytoskeletal components. In Drosophila melanogaster embryos, the first 13 nuclear divisions occur without cytokinesis. During the final four divisions, nuclei divide in a uniform monolayer at the surface of the embryo. These surface divisions are accompanied by dramatic changes in cortical actin and microtubule structure (Karr and Alberts, 1986), and inhibitor studies indicate that these changes are essential to orderly mitosis (Zalokar and Erk, 1976). Because the early embryo is syncytial, fluorescent probes introduced by microinjection are incorporated in structures associated with all of the nuclei in the blastoderm. In addition, the nuclei divide synchronously every 10 to 20 min. These characteristics make the syncytial blastoderm embryo an excellent system for the analysis of mitotic reorganization of both nuclear and cytoskeletal elements. However, the Drosophila embryo is a large cell, and resolution of cytoskeletal filaments and nuclear structure is hampered by out-of focus signal.

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