Quantitative GUS Activity Assay in Intact Plant Tissue

2007 ◽  
Vol 2007 (2) ◽  
pp. pdb.prot4688-pdb.prot4688 ◽  
Author(s):  
M. Blazquez
Keyword(s):  
Plant Tissue ◽  
Gus Activity ◽  
Intact Plant ◽  
Activity Assay

Quantitative GUS Activity Assay of Plant Extracts

2007 ◽  
Vol 2007 (2) ◽  
pp. pdb.prot4690-pdb.prot4690 ◽  
Author(s):  
M. Blazquez
Keyword(s):  
Plant Extracts ◽  
Gus Activity ◽  
Activity Assay

scholarly journals Assessment of a 1H high-resolution magic angle spinning NMR spectroscopy procedure for free sugars quantification in intact plant tissue

Planta ◽  
2013 ◽  
Vol 238 (2) ◽  
pp. 397-413 ◽  
Author(s):  
Teresa Delgado-Goñi ◽  
Sonia Campo ◽  
Juana Martín-Sitjar ◽  
Miquel E. Cabañas ◽  
Blanca San Segundo ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
High Resolution ◽  
Plant Tissue ◽  
Magic Angle Spinning ◽  
Intact Plant ◽  
Magic Angle ◽  
Free Sugars ◽  
Angle Spinning

scholarly journals Purification of Intact Plant Protoplasts by Flotation at 1g

2002 ◽  
Vol 2 ◽  
pp. 1397-1399
Author(s):  
John Graham
Keyword(s):  
Plant Tissue ◽  
Intact Plant ◽  
Low Density ◽  
Plant Protoplasts ◽  
Density Barrier

From a standard plant tissue digest adjusted to a density of 1.07 g/ml, protoplasts can be harvested by flotation through a low density barrier (1.03 g/ml). The delicate nature of these bodies is suited to this flotation strategy which can be carried out at 1g.

scholarly journals Endogenous Fluorescence Identifies Dead Cells In Plants

2001 ◽  
Vol 9 (2) ◽  
pp. 22-24 ◽  
Author(s):  
Jan S. Ryerse ◽  
Paul C. C. Feng ◽  
R. Douglas Sammons
Keyword(s):  
Propidium Iodide ◽  
Plant Tissue ◽  
Intact Plant ◽  
Plant Tissues ◽  
Plant Protoplasts ◽  
Animal Tissues ◽  
Dye Penetration ◽  
Fluorescent Stains ◽  
Endogenous Fluorescence ◽  
Uv Excitation

Various fluorescent stains and vital dyes have been used to identify dead cells in animal tissues and celi lines. In plants, fluorescein diacetate and propidium iodide have been used to label nuclei and to identify necrotic cells in plant protoplasts and 4,6-diamidino-2-phenylindole (DAPI) has been used to mark senescing cells in sections of roots. However, these dyes may be problematic when used with intact plant tissue with well-developed cells walls which may impede dye penetration. Endogenous fluorescence has been used to identify dead cells in intact and sectioned plant tissues. Published procedures typically employ ultraviolet (UV) excitation wavelengths of 340-380 nm and emission wavelengths of 400- 425 nm, thus requiring a UV filter set.

A novel microsurgery method for intact plant tissue at the single cell level using ArF excimer laser microprojection

2006 ◽  
Vol 93 (2) ◽  
pp. 325-331 ◽  
Author(s):  
Shin'ichiro Kajiyama ◽  
Takeshi Shoji ◽  
Shinya Okuda ◽  
Yoshihiro Izumi ◽  
Ei-ichiro Fukusaki ◽  
...  
Keyword(s):  
Single Cell ◽  
Excimer Laser ◽  
Plant Tissue ◽  
Intact Plant ◽  
Single Cell Level ◽  
Cell Level ◽  
Arf Excimer Laser

Use of the lacZ reporter gene as an internal control for GUS activity in microprojectile bombarded plant tissue

Plant Science ◽  
1996 ◽  
Vol 120 (2) ◽  
pp. 153-160 ◽  
Author(s):  
Gillian Hull ◽  
JoséManuel Garcia Garrido ◽  
François Parcy ◽  
Marcelo Menossi ◽  
JoséAntonio Martinez-Izquierdo ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Internal Control ◽  
Plant Tissue ◽  
Gus Activity ◽  
Lacz Reporter ◽  
Lacz Reporter Gene

Freeze-fracture, freeze-etch complementary pairs of virus-infected cells reveal value of etching even when relatively high concentrations of cryoprotectants are used

1978 ◽  
Vol 36 (2) ◽  
pp. 136-137
Author(s):  
Russell L. Steere ◽  
Eric F. Erbe
Keyword(s):  
Plant Tissue ◽  
Phosphate Buffer ◽  
Infected Plant ◽  
Freeze Fracture ◽  
Distilled Water ◽  
Virus Infected Plant ◽  
Infected Cells ◽  
High Concentrations

It has been assumed by many involved in freeze-etch or freeze-fracture studies that it would be useless to etch specimens which were cryoprotected by more than 15% glycerol. We presumed that the amount of cryoprotective material exposed at the surface would serve as a contaminating layer and prevent the visualization of fine details. Recent unexpected freeze-etch results indicated that it would be useful to compare complementary replicas in which one-half of the frozen-fractured specimen would be shadowed and replicated immediately after fracturing whereas the complement would be etched at -98°C for 1 to 10 minutes before being shadowed and replicated.Standard complementary replica holders (Steere, 1973) with hinges removed were used for this study. Specimens consisting of unfixed virus-infected plant tissue infiltrated with 0.05 M phosphate buffer or distilled water were used without cryoprotectant. Some were permitted to settle through gradients to the desired concentrations of different cryoprotectants.

Freeze-Fracture Studies of Membranes in Developing Sieve Elements in a Plant Tissue Culture

1980 ◽  
Vol 38 ◽  
pp. 636-637
Author(s):  
R. D. Sjolund ◽  
C. Y. Shih
Keyword(s):  
Plant Tissue ◽  
Cultured Cells ◽  
Freeze Fracture ◽  
Tissue Cultures ◽  
Sieve Elements ◽  
Intact Plants ◽  
Plant Tissue Cultures ◽  
Whole Plants ◽  
Energy Dependent ◽  
Similar Elements

The differentiation of phloem in plant tissue cultures offers a unique opportunity to study the development and structure of sieve elements in a manner that avoids the injury responses associated with the processing of similar elements in intact plants. Short segments of sieve elements formed in tissue cultures can be fixed intact while the longer strands occuring in whole plants must be cut into shorter lengths before processing. While iyuch controversy surrounds the question of phloem function in tissue cultures , sieve elements formed in these cultured cells are structurally similar to those of Intact plants. We are particullarly Interested In the structure of the plasma membrane and the peripheral ER in these cells because of their possible role in the energy-dependent active transport of sucrose into the sieve elements.

Azocoll protease activity assay

2007 ◽  
Author(s):  
Naxin Jiang ◽  
Nguan Soon Tan ◽  
Bow Ho ◽  
Jeak Ling Ding
Keyword(s):  
Protease Activity ◽  
Activity Assay
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