scholarly journals Purification of Intact Plant Protoplasts by Flotation at 1g

2002 ◽  
Vol 2 ◽  
pp. 1397-1399
Author(s):  
John Graham
Keyword(s):  
Plant Tissue ◽  
Intact Plant ◽  
Low Density ◽  
Plant Protoplasts ◽  
Density Barrier

From a standard plant tissue digest adjusted to a density of 1.07 g/ml, protoplasts can be harvested by flotation through a low density barrier (1.03 g/ml). The delicate nature of these bodies is suited to this flotation strategy which can be carried out at 1g.

scholarly journals Endogenous Fluorescence Identifies Dead Cells In Plants

2001 ◽  
Vol 9 (2) ◽  
pp. 22-24 ◽  
Author(s):  
Jan S. Ryerse ◽  
Paul C. C. Feng ◽  
R. Douglas Sammons
Keyword(s):  
Propidium Iodide ◽  
Plant Tissue ◽  
Intact Plant ◽  
Plant Tissues ◽  
Plant Protoplasts ◽  
Animal Tissues ◽  
Dye Penetration ◽  
Fluorescent Stains ◽  
Endogenous Fluorescence ◽  
Uv Excitation

Various fluorescent stains and vital dyes have been used to identify dead cells in animal tissues and celi lines. In plants, fluorescein diacetate and propidium iodide have been used to label nuclei and to identify necrotic cells in plant protoplasts and 4,6-diamidino-2-phenylindole (DAPI) has been used to mark senescing cells in sections of roots. However, these dyes may be problematic when used with intact plant tissue with well-developed cells walls which may impede dye penetration. Endogenous fluorescence has been used to identify dead cells in intact and sectioned plant tissues. Published procedures typically employ ultraviolet (UV) excitation wavelengths of 340-380 nm and emission wavelengths of 400- 425 nm, thus requiring a UV filter set.

scholarly journals Separation of Monocytes from Whole Human Blood

2002 ◽  
Vol 2 ◽  
pp. 1540-1543
Author(s):  
John Graham
Keyword(s):  
Human Blood ◽  
Whole Blood ◽  
Human Peripheral Blood ◽  
Preliminary Evidence ◽  
Low Density ◽  
Rapid Rate ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Density Barrier ◽  
Edta Anticoagulated Blood

Human peripheral blood monocytes are isolated by flotation from whole blood through a single low-density barrier prepared from OptiPrep™ at 4°C. The separation from lymphocytes depends on the more rapid rate of flotation of the monocytes because of their slightly lower density and larger size. The method works optimally only with fresh (within 2 h of drawing) EDTA-anticoagulated blood. Preliminary evidence suggests that this technique may be applicable to blood from rats.

Quantitative GUS Activity Assay in Intact Plant Tissue

2007 ◽  
Vol 2007 (2) ◽  
pp. pdb.prot4688-pdb.prot4688 ◽  
Author(s):  
M. Blazquez
Keyword(s):  
Plant Tissue ◽  
Gus Activity ◽  
Intact Plant ◽  
Activity Assay

scholarly journals Assessment of a 1H high-resolution magic angle spinning NMR spectroscopy procedure for free sugars quantification in intact plant tissue

Planta ◽  
2013 ◽  
Vol 238 (2) ◽  
pp. 397-413 ◽  
Author(s):  
Teresa Delgado-Goñi ◽  
Sonia Campo ◽  
Juana Martín-Sitjar ◽  
Miquel E. Cabañas ◽  
Blanca San Segundo ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
High Resolution ◽  
Plant Tissue ◽  
Magic Angle Spinning ◽  
Intact Plant ◽  
Magic Angle ◽  
Free Sugars ◽  
Angle Spinning

scholarly journals 384 Modified-Atmosphere Packaging of Fresh Produce to Prevent Generation of Anaerobic Environments

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 510B-510
Author(s):  
Michael Wendorf ◽  
Nazir A. Mir ◽  
Randolph M. Beaudry
Keyword(s):  
Hydrogen Peroxide ◽  
Plant Tissue ◽  
Room Temperature ◽  
Storage Temperature ◽  
Modified Atmosphere Packaging ◽  
Low Density Polyethylene ◽  
Modified Atmosphere ◽  
Low Density ◽  
Aerobic Conditions ◽  
Ambient Room Temperature

Broccoli tissue, ranging in weight from 7 to 21 g, was sealed in packages made from low-density polyethylene (LDPE) of various thickness and permeability to establish a range of O2 levels in the package headspace. A pouch containing either hydrogen peroxide (H2O2) or water as a control was also sealed in the package. For packages that developed anaerobic atmospheres, inclusion of H2O2 permitted the maintenance of aerobic conditions for up to 3 days at ambient room temperature. These results suggest that the plant tissue is able to actively metabolize the H2O2 vapor to generate O2, which will prevent the development of low-O2 conditions in packaged produce, even under conditions of elevated storage temperature.

scholarly journals Isolation of a Mouse Motoneuron-Enriched Fraction from Mouse Spinal Cord on a Density Barrier

2002 ◽  
Vol 2 ◽  
pp. 1544-1546 ◽  
Author(s):  
John Graham
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Tissue ◽  
Low Density ◽  
Mouse Spinal Cord ◽  
Mechanical Disruption ◽  
Density Barrier

After a combined enzymic and mechanical disruption of the spinal cord tissue, the low-density motoneurons band at the interface of a 1.06-g/ml barrier through which other contaminating cells sediment.

A novel microsurgery method for intact plant tissue at the single cell level using ArF excimer laser microprojection

2006 ◽  
Vol 93 (2) ◽  
pp. 325-331 ◽  
Author(s):  
Shin'ichiro Kajiyama ◽  
Takeshi Shoji ◽  
Shinya Okuda ◽  
Yoshihiro Izumi ◽  
Ei-ichiro Fukusaki ◽  
...  
Keyword(s):  
Single Cell ◽  
Excimer Laser ◽  
Plant Tissue ◽  
Intact Plant ◽  
Single Cell Level ◽  
Cell Level ◽  
Arf Excimer Laser

scholarly journals Separation of Membrane Vesicles and Cytosol from Cultured Cells and Bacteria in a Preformed Discontinuous Gradient

2002 ◽  
Vol 2 ◽  
pp. 1555-1559 ◽  
Author(s):  
John Graham
Keyword(s):  
Opposite Direction ◽  
Cultured Cells ◽  
Membrane Vesicle ◽  
Membrane Vesicles ◽  
Complete Separation ◽  
Low Density ◽  
Sample Zone ◽  
Low Viscosity ◽  
Density Barrier

There are many situations when it is necessary to separate rapidly and efficiently a cytosolic and a membrane vesicle fraction from either cultured cells or from bacteria. Flotation of the vesicles through a low-density barrier from a dense sample zone using the low viscosity medium iodixanol allows complete separation of these compartments. As the sample is exposed to the gmax the tendency of the proteins to sediment overcomes any diffusion in the opposite direction.

scholarly journals Fractionation of Hepatic Nonparenchymal Cells

2002 ◽  
Vol 2 ◽  
pp. 1347-1350 ◽  
Author(s):  
John Graham
Keyword(s):  
Endothelial Cells ◽  
Stellate Cells ◽  
Cell Types ◽  
Low Density ◽  
Nonparenchymal Cells ◽  
Mammalian Liver ◽  
Hepatic Nonparenchymal Cells ◽  
Density Barrier ◽  
Parenchymal Cells ◽  
Different Cell Types

The majority of parenchymal cells from mammalian liver cells can be removed by very low speed centrifugation (50 g) but a simple low-density barrier (1.096 g/ml) is required to remove the remaining parenchymal cells from the 50-g supernatant which contains all of the lower density nonparenchymal cells. Continuous gradients of Nycodenz®can provide satisfactory resolution of Kupffer, stellate, and endothelial cells on an analytical basis but the separation of different cell types is not sufficient preparatively. Flotation through a low-density iodixanol barrier can, however, provide a satisfactory enrichment of the least dense nonparenchymal cell – the stellate cells.

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