Preparing Frozen Tissue Sections for Immunostaining

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot4328
Author(s):  
Ed Harlow ◽  
David Lane
Keyword(s):  
Tissue Sections ◽  
Frozen Tissue

scholarly journals Rapid brain structure and tumour margin detection on whole frozen tissue sections by fast multiphotometric mid-infrared scanning

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tim Kümmel ◽  
Björn van Marwick ◽  
Miriam Rittel ◽  
Carina Ramallo Guevara ◽  
Felix Wühler ◽  
...  
Keyword(s):  
Imaging System ◽  
Frozen Section ◽  
Computational Effort ◽  
Primary Hepatocellular Carcinoma ◽  
Measuring System ◽  
Sufficient Information ◽  
Tissue Samples ◽  
Mid Infrared ◽  
Tissue Sections ◽  
Frozen Tissue

AbstractFrozen section analysis is a frequently used method for examination of tissue samples, especially for tumour detection. In the majority of cases, the aim is to identify characteristic tissue morphologies or tumour margins. Depending on the type of tissue, a high number of misdiagnoses are associated with this process. In this work, a fast spectroscopic measurement device and workflow was developed that significantly improves the speed of whole frozen tissue section analyses and provides sufficient information to visualize tissue structures and tumour margins, dependent on their lipid and protein molecular vibrations. That optical and non-destructive method is based on selected wavenumbers in the mid-infrared (MIR) range. We present a measuring system that substantially outperforms a commercially available Fourier Transform Infrared (FT-IR) Imaging system, since it enables acquisition of reduced spectral information at a scan field of 1 cm2 in 3 s, with a spatial resolution of 20 µm. This allows fast visualization of segmented structure areas with little computational effort. For the first time, this multiphotometric MIR system is applied to biomedical tissue sections. We are referencing our novel MIR scanner on cryopreserved murine sagittal and coronal brain sections, especially focusing on the hippocampus, and show its usability for rapid identification of primary hepatocellular carcinoma (HCC) in mouse liver.

Raman molecular imaging of brain frozen tissue sections

2014 ◽  
Vol 120 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Rachel E. Kast ◽  
Gregory W. Auner ◽  
Mark L. Rosenblum ◽  
Tom Mikkelsen ◽  
Sally M. Yurgelevic ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Tissue Sections ◽  
Frozen Tissue

Surface immunoglobulin restriction in B-non Hodgkin's lymphomas in cell suspension and on frozen tissue sections

2009 ◽  
Vol 35 (4) ◽  
pp. 399-407 ◽  
Author(s):  
Philip M. Kluin ◽  
Roel A. Weger ◽  
Henk-Jan Schuurman ◽  
Peter P. J. Peters ◽  
Philomé I. Spies ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Tissue Sections ◽  
Surface Immunoglobulin ◽  
Frozen Tissue ◽  
Hodgkin's Lymphomas

scholarly journals Microdissection RT-PCR Analysis of Gene Expression in Pathologically Defined Frozen Tissue Sections

BioTechniques ◽  
1996 ◽  
Vol 21 (1) ◽  
pp. 38-44 ◽  
Author(s):  
Tamara Hiller ◽  
Linda Snell ◽  
Peter H. Watson
Keyword(s):  
Gene Expression ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Tissue Sections ◽  
Frozen Tissue

scholarly journals Brief Report: Simplified Myeloperoxidase Stain Using Benzidine Dihydrochloride

Blood ◽  
1965 ◽  
Vol 26 (2) ◽  
pp. 215-219 ◽  
Author(s):  
LEONARD S. KAPLOW ◽  
Charlotte Ladd
Keyword(s):  
Peroxidase Activity ◽  
Incubation Mixture ◽  
Tissue Sections ◽  
Blood Smears ◽  
Highly Sensitive ◽  
Frozen Tissue ◽  
Fresh Frozen

Abstract A method is described for demonstrating leukocyte peroxidase activity in which benzidine dihydrochloride is used as the indicator compound instead of the more commonly used but potentially more hazardous benzidine base. The method is highly sensitive and rapid and permits the use of fixed blood smears and organ imprints. The incubation mixture, which incorporates safranin as a counterstain, may be used over and over again, for as long as 6 months. The method is also applicable to fresh frozen tissue sections.

Histochemical demonstration of hydrogen peroxide production by leukocytes in fixed-frozen tissue sections of inflammatory lesions

1994 ◽  
Vol 56 (4) ◽  
pp. 436-443 ◽  
Author(s):  
Arthur M. Dannenberg ◽  
Brian H. Schofield ◽  
Jay B. Rao ◽  
Theresa T. Dinh ◽  
Ki Lee ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Histochemical Demonstration ◽  
Hydrogen Peroxide Production ◽  
Inflammatory Lesions ◽  
Tissue Sections ◽  
Frozen Tissue

Tissue distribution of sulfolipids in the rat. Restricted location of sulfatoxygalactosylacylalkylglyerol

1981 ◽  
Vol 59 (7) ◽  
pp. 556-563 ◽  
Author(s):  
Clifford Lingwood ◽  
Genevieve Hay ◽  
Harry Schachter
Keyword(s):  
Tissue Distribution ◽  
Indirect Immunofluorescence ◽  
Rat Tissues ◽  
Tissue Sections ◽  
Frozen Tissue

Eleven rat tissues (excluding brain) have been assayed for their ability to synthesize sulfatoxygalactosylacylalkylglycerol (SGG) from Na235SO4in vivo. These tissues were also assayed for the presence of SGG by an indirect immunofluorescence procedure using rabbit anti-SGG and frozen tissue sections. By both procedures SGG was found to be restricted to the testis; several novel sulfolipids were detected during this study.

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