Inducing Yeast Cell Synchrony: Dilution of Stationary-Phase Cultures

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot4176
Author(s):  
David C. Amberg ◽  
Daniel J. Burke ◽  
Jeffrey N. Strathern
Keyword(s):  
Stationary Phase ◽  
Yeast Cell ◽  
Cell Synchrony

scholarly journals The rye Mutants Identify a Role for Ssn/Srb Proteins of the RNA Polymerase II Holoenzyme During Stationary Phase Entry in Saccharomyces cerevisiae

Genetics ◽  
2001 ◽  
Vol 157 (1) ◽  
pp. 17-26 ◽  
Author(s):  
Ya-Wen Chang ◽  
Susie C Howard ◽  
Yelena V Budovskaya ◽  
Jasper Rine ◽  
Paul K Herman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Growth ◽  
Rna Polymerase ◽  
Stationary Phase ◽  
Yeast Cell ◽  
Rna Polymerase Ii ◽  
Transcriptional Response ◽  
Nutrient Deprivation ◽  
Ras Signaling ◽  
Rna Polymerase Ii Holoenzyme

Abstract Saccharomyces cerevisiae cells enter into a distinct resting state, known as stationary phase, in response to specific types of nutrient deprivation. We have identified a collection of mutants that exhibited a defective transcriptional response to nutrient limitation and failed to enter into a normal stationary phase. These rye mutants were isolated on the basis of defects in the regulation of YGP1 expression. In wild-type cells, YGP1 levels increased during the growth arrest caused by nutrient deprivation or inactivation of the Ras signaling pathway. In contrast, the levels of YGP1 and related genes were significantly elevated in the rye mutants during log phase growth. The rye defects were not specific to this YGP1 response as these mutants also exhibited multiple defects in stationary phase properties, including an inability to survive periods of prolonged starvation. These data indicated that the RYE genes might encode important regulators of yeast cell growth. Interestingly, three of the RYE genes encoded the Ssn/Srb proteins, Srb9p, Srb10p, and Srb11p, which are associated with the RNA polymerase II holoenzyme. Thus, the RNA polymerase II holoenzyme may be a target of the signaling pathways responsible for coordinating yeast cell growth with nutrient availability.

scholarly journals Enhancement of plasmid DNA transformation efficiencies in early stationary-phase yeast cell cultures

Yeast ◽  
2013 ◽  
Vol 30 (5) ◽  
pp. 191-200 ◽  
Author(s):  
Jennifer DeMars Tripp ◽  
Jennifer L. Lilley ◽  
Whitney N. Wood ◽  
L. Kevin Lewis
Keyword(s):  
Stationary Phase ◽  
Yeast Cell ◽  
Cell Cultures ◽  
Plasmid Dna ◽  
Dna Transformation ◽  
Early Stationary Phase

Inducing Yeast Cell Synchrony: Hydroxyurea Arrest

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot4174
Author(s):  
David C. Amberg ◽  
Daniel J. Burke ◽  
Jeffrey N. Strathern
Keyword(s):  
Yeast Cell ◽  
Cell Synchrony

Inducing Yeast Cell Synchrony: Nocodazole Arrest

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot4175
Author(s):  
David C. Amberg ◽  
Daniel J. Burke ◽  
Jeffrey N. Strathern
Keyword(s):  
Yeast Cell ◽  
Cell Synchrony

Inducing Yeast Cell Synchrony: α-Factor Arrest Usingbar1Mutants

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot4173 ◽  
Author(s):  
David C. Amberg ◽  
Daniel J. Burke ◽  
Jeffrey N. Strathern
Keyword(s):  
Yeast Cell ◽  
Cell Synchrony

Inducing Yeast Cell Synchrony: α-Factor Arrest Using Low Cell Concentration

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot4171
Author(s):  
David C. Amberg ◽  
Daniel J. Burke ◽  
Jeffrey N. Strathern
Keyword(s):  
Yeast Cell ◽  
Cell Concentration ◽  
Cell Synchrony

scholarly journals Requirement of peroxiredoxin on the stationary phase of yeast cell growth

2014 ◽  
Vol 39 (1) ◽  
pp. 51-58 ◽  
Author(s):  
Toshihiko Watanabe ◽  
Hayato Irokawa ◽  
Ayako Ogasawara ◽  
Kenta Iwai ◽  
Shusuke Kuge
Keyword(s):  
Cell Growth ◽  
Stationary Phase ◽  
Yeast Cell

Inducing Yeast Cell Synchrony: α-Factor Arrest Using Low pH

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot4172 ◽  
Author(s):  
David C. Amberg ◽  
Daniel J. Burke ◽  
Jeffrey N. Strathern
Keyword(s):  
Yeast Cell ◽  
Low Ph ◽  
Cell Synchrony
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