scholarly journals Involvement ofGDH3-encoded NADP+-dependent Glutamate Dehydrogenase in Yeast Cell Resistance to Stress-induced Apoptosis in Stationary Phase Cells

2012 ◽  
Vol 287 (53) ◽  
pp. 44221-44233 ◽  
Author(s):  
Yong Joo Lee ◽  
Kyung Jin Kim ◽  
Hong Yong Kang ◽  
Hye-Rim Kim ◽  
Pil Jae Maeng
Keyword(s):  
Stationary Phase ◽  
Yeast Cell ◽  
Glutamate Dehydrogenase ◽  
Cell Resistance ◽  
Resistance To Stress ◽  
Induced Apoptosis

scholarly journals The rye Mutants Identify a Role for Ssn/Srb Proteins of the RNA Polymerase II Holoenzyme During Stationary Phase Entry in Saccharomyces cerevisiae

Genetics ◽  
2001 ◽  
Vol 157 (1) ◽  
pp. 17-26 ◽  
Author(s):  
Ya-Wen Chang ◽  
Susie C Howard ◽  
Yelena V Budovskaya ◽  
Jasper Rine ◽  
Paul K Herman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Growth ◽  
Rna Polymerase ◽  
Stationary Phase ◽  
Yeast Cell ◽  
Rna Polymerase Ii ◽  
Transcriptional Response ◽  
Nutrient Deprivation ◽  
Ras Signaling ◽  
Rna Polymerase Ii Holoenzyme

Abstract Saccharomyces cerevisiae cells enter into a distinct resting state, known as stationary phase, in response to specific types of nutrient deprivation. We have identified a collection of mutants that exhibited a defective transcriptional response to nutrient limitation and failed to enter into a normal stationary phase. These rye mutants were isolated on the basis of defects in the regulation of YGP1 expression. In wild-type cells, YGP1 levels increased during the growth arrest caused by nutrient deprivation or inactivation of the Ras signaling pathway. In contrast, the levels of YGP1 and related genes were significantly elevated in the rye mutants during log phase growth. The rye defects were not specific to this YGP1 response as these mutants also exhibited multiple defects in stationary phase properties, including an inability to survive periods of prolonged starvation. These data indicated that the RYE genes might encode important regulators of yeast cell growth. Interestingly, three of the RYE genes encoded the Ssn/Srb proteins, Srb9p, Srb10p, and Srb11p, which are associated with the RNA polymerase II holoenzyme. Thus, the RNA polymerase II holoenzyme may be a target of the signaling pathways responsible for coordinating yeast cell growth with nutrient availability.

scholarly journals Mesothelin confers pancreatic cancer cell resistance to TNF-α-induced apoptosis through Akt/PI3K/NF-κB activation and IL-6/Mcl-1 overexpression

2011 ◽  
Vol 10 (1) ◽  
pp. 106 ◽  
Author(s):  
Uddalak Bharadwaj ◽  
Christian Marin-Muller ◽  
Min Li ◽  
Changyi Chen ◽  
Qizhi Yao
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cell ◽  
Pancreatic Cancer Cell ◽  
Cell Resistance ◽  
Tnf Α ◽  
Induced Apoptosis

scholarly journals Cell death in the avian blastoderm: resistance to stress-induced apoptosis and expression of anti-apoptotic genes

1998 ◽  
Vol 5 (6) ◽  
pp. 529-538 ◽  
Author(s):  
Stephen E Bloom ◽  
Donna E Muscarella ◽  
Mitchell Y Lee ◽  
Melissa Rachlinski
Keyword(s):  
Cell Death ◽  
Resistance To Stress ◽  
Apoptotic Genes ◽  
Induced Apoptosis

Inducing Yeast Cell Synchrony: Dilution of Stationary-Phase Cultures

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot4176
Author(s):  
David C. Amberg ◽  
Daniel J. Burke ◽  
Jeffrey N. Strathern
Keyword(s):  
Stationary Phase ◽  
Yeast Cell ◽  
Cell Synchrony

scholarly journals Enhancement of plasmid DNA transformation efficiencies in early stationary-phase yeast cell cultures

Yeast ◽  
2013 ◽  
Vol 30 (5) ◽  
pp. 191-200 ◽  
Author(s):  
Jennifer DeMars Tripp ◽  
Jennifer L. Lilley ◽  
Whitney N. Wood ◽  
L. Kevin Lewis
Keyword(s):  
Stationary Phase ◽  
Yeast Cell ◽  
Cell Cultures ◽  
Plasmid Dna ◽  
Dna Transformation ◽  
Early Stationary Phase

Tumor-cell resistance to death receptor–induced apoptosis through mutational inactivation of the proapoptotic Bcl-2 homolog Bax

2002 ◽  
Vol 8 (3) ◽  
pp. 274-281 ◽  
Author(s):  
Heidi LeBlanc ◽  
David Lawrence ◽  
Eugene Varfolomeev ◽  
Klara Totpal ◽  
John Morlan ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Death Receptor ◽  
Cell Resistance ◽  
Induced Apoptosis ◽  
Tumor Cell Resistance

scholarly journals The Legionella pneumophila rpoS Gene Is Required for Growth within Acanthamoeba castellanii

1999 ◽  
Vol 181 (16) ◽  
pp. 4879-4889 ◽  
Author(s):  
Laura M. Hales ◽  
Howard A. Shuman
Keyword(s):  
Stationary Phase ◽  
Mutant Strain ◽  
Legionella Pneumophila ◽  
Regulatory Networks ◽  
Acanthamoeba Castellanii ◽  
Logarithmic Phase ◽  
Insertion Mutation ◽  
Wild Type ◽  
E Coli ◽  
Resistance To Stress

ABSTRACT To investigate regulatory networks in Legionella pneumophila, the gene encoding the homolog of theEscherichia coli stress and stationary-phase sigma factor RpoS was identified by complementation of an E. coli rpoSmutation. An open reading frame that is approximately 60% identical to the E. coli rpoS gene was identified. Western blot analysis showed that the level of L. pneumophila RpoS increased in stationary phase. An insertion mutation was constructed in therpoS gene on the chromosome of L. pneumophila, and the ability of this mutant strain to survive various stress conditions was assayed and compared with results for the wild-type strain. Both the mutant and wild-type strains were more resistant to stress when in stationary phase than when in the logarithmic phase of growth. This finding indicates that L. pneumophila RpoS is not required for a stationary-phase-dependent resistance to stress. Although the mutant strain was able to kill HL-60- and THP-1-derived macrophages, it could not replicate within a protozoan host,Acanthamoeba castellanii. These data suggest that L. pneumophila possesses a growth phase-dependent resistance to stress that is independent of RpoS control and that RpoS likely regulates genes that enable it to survive in the environment within protozoa. Our data indicate that the role of rpoS inL. pneumophila is very different from what has previously been reported for E. coli rpoS.

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