Monitoring Tectal Neuronal Activities and Motor Behavior in Zebrafish Larvae

Germán Sumbre; Mu-Ming Poo

doi:10.1101/pdb.prot077131

Molecular and cellular determinants of motor asymmetry in zebrafish

10.1101/666594 ◽

2019 ◽

Cited By ~ 1

Author(s):

Eric J. Horstick ◽

Yared Bayleyen ◽

Harold A. Burgess

Keyword(s):

Visual Information ◽

Motor Behavior ◽

Hand Preference ◽

Neural Substrates ◽

Developmental Pathways ◽

Human Hand ◽

Motor Asymmetry ◽

Zebrafish Larvae ◽

Developmental Signaling ◽

Functional Lateralization

AbstractAsymmetries in motor behavior, such as human hand preference, are observed throughout bilateria. However, neural substrates and developmental signaling pathways that impose underlying functional lateralization on a broadly symmetric nervous system are unknown. Here we report that in the absence of over-riding visual information, zebrafish larvae show intrinsic lateralized motor behavior that is mediated by a cluster of 60 posterior tuberculum (PT) neurons in the forebrain. PT neurons impose motor bias via a projection through the epithalamic commissure to the habenula. Acquisition of left/right identity is disrupted by heterozygous mutations in mosaic eyes and mindbomb, genes that regulate Notch signaling. These results define the neuronal substrate for motor asymmetry in a vertebrate and support the idea that developmental pathways that establish visceral asymmetries also govern acquisition of left/right identity.

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Dopamine D2 Receptor Activity Modulates Akt Signaling and Alters GABAergic Neuron Development and Motor Behavior in Zebrafish Larvae

Journal of Neuroscience ◽

10.1523/jneurosci.5548-10.2011 ◽

2011 ◽

Vol 31 (14) ◽

pp. 5512-5525 ◽

Cited By ~ 48

Author(s):

B. R. Souza ◽

M. A. Romano-Silva ◽

V. Tropepe

Keyword(s):

Motor Behavior ◽

Dopamine D2 Receptor ◽

D2 Receptor ◽

Akt Signaling ◽

Gabaergic Neuron ◽

Receptor Activity ◽

Neuron Development ◽

Zebrafish Larvae ◽

Dopamine D2

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Functional magnetic resonance imaging of human brain during motor behavior

Electroencephalography and Clinical Neurophysiology ◽

10.1016/s0013-4694(97)88159-3 ◽

1997 ◽

Vol 103 (1) ◽

pp. 56

Author(s):

J Sanes

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Functional Magnetic Resonance Imaging ◽

Human Brain ◽

Motor Behavior ◽

Functional Magnetic Resonance ◽

Resonance Imaging

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Behavior in the Brain

Journal of Psychophysiology ◽

10.1027/0269-8803/a000016 ◽

2010 ◽

Vol 24 (2) ◽

pp. 76-82 ◽

Cited By ~ 15

Author(s):

Martin M. Monti ◽

Adrian M. Owen

Keyword(s):

Motor Behavior ◽

Functional Neuroimaging ◽

Brain Activity ◽

Motor Output ◽

The Unconscious ◽

Ethical Implications ◽

Brain Responses ◽

Minimally Conscious ◽

Brain Insults ◽

Brain Behavior

Recent evidence has suggested that functional neuroimaging may play a crucial role in assessing residual cognition and awareness in brain injury survivors. In particular, brain insults that compromise the patient’s ability to produce motor output may render standard clinical testing ineffective. Indeed, if patients were aware but unable to signal so via motor behavior, they would be impossible to distinguish, at the bedside, from vegetative patients. Considering the alarming rate with which minimally conscious patients are misdiagnosed as vegetative, and the severe medical, legal, and ethical implications of such decisions, novel tools are urgently required to complement current clinical-assessment protocols. Functional neuroimaging may be particularly suited to this aim by providing a window on brain function without requiring patients to produce any motor output. Specifically, the possibility of detecting signs of willful behavior by directly observing brain activity (i.e., “brain behavior”), rather than motoric output, allows this approach to reach beyond what is observable at the bedside with standard clinical assessments. In addition, several neuroimaging studies have already highlighted neuroimaging protocols that can distinguish automatic brain responses from willful brain activity, making it possible to employ willful brain activations as an index of awareness. Certainly, neuroimaging in patient populations faces some theoretical and experimental difficulties, but willful, task-dependent, brain activation may be the only way to discriminate the conscious, but immobile, patient from the unconscious one.

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