scholarly journals A systematic model to predict transcriptional regulatory mechanisms based on overrepresentation of transcription factor binding profiles

2006 ◽  
Vol 16 (3) ◽  
pp. 405-413 ◽  
Author(s):  
L.-W. Chang
Keyword(s):  
Transcription Factor ◽  
Transcription Factor Binding ◽  
Regulatory Mechanisms ◽  
Factor Binding ◽  
Transcriptional Regulatory ◽  
Transcriptional Regulatory Mechanisms

scholarly journals HbWRKY27, a group IIe WRKY transcription factor, positively regulates HbFPS1 expression in Hevea brasiliensis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Long Qu ◽  
Hui-Liang Li ◽  
Dong Guo ◽  
Ying Wang ◽  
Jia-Hong Zhu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Hevea Brasiliensis ◽  
Tobacco Plant ◽  
Wrky Transcription Factor ◽  
Regulatory Mechanisms ◽  
Farnesyl Pyrophosphate ◽  
Rubber Biosynthesis ◽  
Transcriptional Regulatory ◽  
Key Enzyme ◽  
Transcriptional Regulatory Mechanisms

AbstractFarnesyl pyrophosphate synthase (FPS) is a key enzyme that catalyzes the formation of farnesyl pyrophosphate, the main initiator for rubber chain initiation in Hevea brasiliensis Muell. Arg. The transcriptional regulatory mechanisms of the FPS gene still not well understood. Here, a WRKY transcription factor designated HbWRKY27 was obtained by screening the latex cDNA library applied the HbFPS1 promoter as bait. HbWRKY27 interacted with the HbFPS1 promoter was further identified by individual Y1H and EMSA assays. HbWRKY27 belongs to group IIe WRKY subfamily which contains a typical WRKY domain and C-X5-CX23-HXH motif. HbWRKY27 was localized to the nucleus. HbWRKY27 predominantly accumulated in latex. HbWRKY27 was up-regulated in latex by ethrel, salicylic acid, abscisic acid, and methyl jasmonate treatment. Transient expression of HbWRKY27 led to increasing the activity of the HbFPS1 promoter in tobacco plant, suggesting that HbWRKY27 positively regulates the HbFPS1 expression. Taken together, an upstream transcription factor of the key natural rubber biosynthesis gene HbFPS1 was identified and this study will provide novel transcriptional regulatory mechanisms of the FPS gene in Hevea brasiliensis.

scholarly journals Interplay of cis and trans mechanisms driving transcription factor binding and gene expression evolution

2016 ◽  
Author(s):  
Emily S Wong ◽  
Bianca M Schmitt ◽  
Anastasiya Kazachenka ◽  
David Thybert ◽  
Aisling Redmond ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Inbred Mouse ◽  
Transcription Factor Binding ◽  
Regulatory Mechanisms ◽  
Mouse Strains ◽  
Cis Acting ◽  
Factor Binding ◽  
Regulatory Variants ◽  
Transcription Factor Occupancy

AbstractNoncoding regulatory variants play a central role in the genetics of human diseases and in evolution. Here we measure allele-specific transcription factor binding occupancy of three liver-specific transcription factors between crosses of two inbred mouse strains to elucidate the regulatory mechanisms underlying transcription factor binding variations in mammals. Our results highlight the pre-eminence of cis-acting variants on transcription factor occupancy divergence. Transcription factor binding differences linked to cis-acting variants generally exhibit additive inheritance, while those linked to trans-acting variants are most often dominantly inherited. Cis-acting variants lead to local coordination of transcription factor occupancies that decay with distance; distal coordination is also observed and may be modulated by long-range chromatin contacts. Our results reveal the regulatory mechanisms that interplay to drive transcription factor occupancy, chromatin state, and gene expression in complex mammalian cell states.

Sign in / Sign up

Export Citation Format

Share Document