scholarly journals Quantitative analysis of specific mRNA transcripts using a competitive PCR assay with electrochemiluminescent detection.

1995 ◽  
Vol 4 (6) ◽  
pp. 327-330 ◽  
Author(s):  
J A Heroux ◽  
A M Szczepanik
Keyword(s):  
Quantitative Analysis ◽  
Competitive Pcr ◽  
Pcr Assay ◽  
Mrna Transcripts ◽  
Specific Mrna

scholarly journals Quantitation of beta-actin-specific mRNA transcripts using xeno-competitive PCR.

1993 ◽  
Vol 3 (1) ◽  
pp. 57-59 ◽  
Author(s):  
R M du Breuil ◽  
J M Patel ◽  
B V Mendelow
Keyword(s):  
Competitive Pcr ◽  
Beta Actin ◽  
Mrna Transcripts ◽  
Specific Mrna

scholarly journals Changes in Expression of Specific mRNA Transcripts after Single- or Re-Irradiation in Mouse Testes

Genes ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 151
Author(s):  
Kenta Nagahori ◽  
Ning Qu ◽  
Miyuki Kuramasu ◽  
Yuki Ogawa ◽  
Daisuke Kiyoshima ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Germ Cell ◽  
Sertoli Cell ◽  
Alkylating Agents ◽  
Mrna Species ◽  
Germ Cell Differentiation ◽  
Targeted Treatments ◽  
Mrna Transcripts ◽  
Specific Mrna

Alkylating agents and irradiation induce testicular damage, which results in prolonged azoospermia. Even very low doses of radiation can significantly impair testis function. However, re-irradiation is an effective strategy for locally targeted treatments and the pain response and has seen important advances in the field of radiation oncology. At present, little is known about the relationship between the harmful effects and accumulated dose of irradiation derived from continuous low-dose radiation exposure. In this study, we examined the levels of mRNA transcripts encoding markers of 13 markers of germ cell differentiation and 28 Sertoli cell-specific products in single- and re-irradiated mice. Our results demonstrated that re-irradiation induced significantly decreased testicular weights with a significant decrease in germ cell differentiation mRNA species (Spo11, Tnp1, Gfra1, Oct4, Sycp3, Ddx4, Boll, Crem, Prm1, and Acrosin). In the 13 Sertoli cell-specific mRNA species decreased upon irradiation, six mRNA species (Claudin-11,Espn, Fshr, GATA1, Inhbb, and Wt1) showed significant differences between single- and re-irradiation. At the same time, different decreases in Sertoli cell-specific mRNA species were found in single-irradiation (Aqp8, Clu, Cst12, and Wnt5a) and re-irradiation (Tjp1, occludin,ZO-1, and ZO-2) mice. These results indicate that long-term aspermatogenesis may differ after single- and re-irradiated treatment.

scholarly journals Effect of Stress on the Ability of a phlA-Based Quantitative Competitive PCR Assay To Monitor Biocontrol Strain Pseudomonas fluorescens CHA0

2003 ◽  
Vol 69 (1) ◽  
pp. 686-690 ◽  
Author(s):  
Fabio Rezzonico ◽  
Yvan Moënne-Loccoz ◽  
Geneviève Défago
Keyword(s):  
Pseudomonas Fluorescens ◽  
Cell Number ◽  
Total Cell ◽  
Competitive Pcr ◽  
Pcr Assay ◽  
Total Cell Number ◽  
Stressed Cells ◽  
Positive Correlations ◽  
Biocontrol Strain

ABSTRACT A quantitative competitive PCR (QC-PCR) assay targeting the phlA gene of Pseudomonas fluorescens CHA0 was developed and tested in vitro. Statistically significant, positive correlations were found between QC-PCR and both CFU and total cell number when studying cells in log or stationary phase. The correlations disappeared when considering stressed cells.

Abundance of specific mRNA transcripts impacts hatching success in European eel, Anguilla anguilla L

2016 ◽  
Vol 191 ◽  
pp. 59-65 ◽  
Author(s):  
Christoffer Rozenfeld ◽  
Ian A.E. Butts ◽  
Jonna Tomkiewicz ◽  
Jose-Luis Zambonino-Infante ◽  
David Mazurais
Keyword(s):  
Hatching Success ◽  
Anguilla Anguilla ◽  
European Eel ◽  
Mrna Transcripts ◽  
Specific Mrna

Helicobacter pyloridetection in human biopsies: a competitive PCR assay with internal control reveals false results

1999 ◽  
Vol 24 (2) ◽  
pp. 201-208 ◽  
Author(s):  
A.-C.E. Thoreson ◽  
M. Borre ◽  
L.P. Andersen ◽  
F. Jørgensen ◽  
S. Kiilerich ◽  
...  
Keyword(s):  
Internal Control ◽  
Competitive Pcr ◽  
Pcr Assay

Quantitative analysis of specific mRNA species in minute cell samples by RT-PCR and flow cytometry

2001 ◽  
Vol 249 (1-2) ◽  
pp. 223-233 ◽  
Author(s):  
Veronika Stemme ◽  
Lars Rymo ◽  
Bo Risberg ◽  
Sten Stemme
Keyword(s):  
Flow Cytometry ◽  
Quantitative Analysis ◽  
Rt Pcr ◽  
Mrna Species ◽  
Specific Mrna
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