scholarly journals Quantitation of beta-actin-specific mRNA transcripts using xeno-competitive PCR.

1993 ◽  
Vol 3 (1) ◽  
pp. 57-59 ◽  
Author(s):  
R M du Breuil ◽  
J M Patel ◽  
B V Mendelow
Keyword(s):  
Competitive Pcr ◽  
Beta Actin ◽  
Mrna Transcripts ◽  
Specific Mrna

scholarly journals Changes in Expression of Specific mRNA Transcripts after Single- or Re-Irradiation in Mouse Testes

Genes ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 151
Author(s):  
Kenta Nagahori ◽  
Ning Qu ◽  
Miyuki Kuramasu ◽  
Yuki Ogawa ◽  
Daisuke Kiyoshima ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Germ Cell ◽  
Sertoli Cell ◽  
Alkylating Agents ◽  
Mrna Species ◽  
Germ Cell Differentiation ◽  
Targeted Treatments ◽  
Mrna Transcripts ◽  
Specific Mrna

Alkylating agents and irradiation induce testicular damage, which results in prolonged azoospermia. Even very low doses of radiation can significantly impair testis function. However, re-irradiation is an effective strategy for locally targeted treatments and the pain response and has seen important advances in the field of radiation oncology. At present, little is known about the relationship between the harmful effects and accumulated dose of irradiation derived from continuous low-dose radiation exposure. In this study, we examined the levels of mRNA transcripts encoding markers of 13 markers of germ cell differentiation and 28 Sertoli cell-specific products in single- and re-irradiated mice. Our results demonstrated that re-irradiation induced significantly decreased testicular weights with a significant decrease in germ cell differentiation mRNA species (Spo11, Tnp1, Gfra1, Oct4, Sycp3, Ddx4, Boll, Crem, Prm1, and Acrosin). In the 13 Sertoli cell-specific mRNA species decreased upon irradiation, six mRNA species (Claudin-11,Espn, Fshr, GATA1, Inhbb, and Wt1) showed significant differences between single- and re-irradiation. At the same time, different decreases in Sertoli cell-specific mRNA species were found in single-irradiation (Aqp8, Clu, Cst12, and Wnt5a) and re-irradiation (Tjp1, occludin,ZO-1, and ZO-2) mice. These results indicate that long-term aspermatogenesis may differ after single- and re-irradiated treatment.

Abundance of specific mRNA transcripts impacts hatching success in European eel, Anguilla anguilla L

2016 ◽  
Vol 191 ◽  
pp. 59-65 ◽  
Author(s):  
Christoffer Rozenfeld ◽  
Ian A.E. Butts ◽  
Jonna Tomkiewicz ◽  
Jose-Luis Zambonino-Infante ◽  
David Mazurais
Keyword(s):  
Hatching Success ◽  
Anguilla Anguilla ◽  
European Eel ◽  
Mrna Transcripts ◽  
Specific Mrna

scholarly journals Changes in Expression of Specific mRNA Transcripts after Single- or Re-Irradiation in Mouse Testes

Genes ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 146
Author(s):  
Kenta Nagahori ◽  
Ning Qu ◽  
Miyuki Kuramasu ◽  
Yuki Ogawa ◽  
Daisuke Kiyoshima ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Germ Cell ◽  
Sertoli Cell ◽  
Alkylating Agents ◽  
Mrna Species ◽  
Germ Cell Differentiation ◽  
Targeted Treatments ◽  
Mrna Transcripts ◽  
Specific Mrna

Alkylating agents and irradiation induce testicular damage, which results in prolonged azoospermia. Even very low doses of radiation can significantly impair testis function. However, re-irradiation is an effective strategy for locally targeted treatments and the pain response and has seen important advances in the field of radiation oncology. At present, little is known about the relationship between the harmful effects and accumulated dose of irradiation derived from continuous low-dose radiation exposure. In this study, we examined the levels of mRNA transcripts encoding markers of 13 markers of germ cell differentiation and 28 Sertoli cell-specific products in single- and re-irradiated mice. Our results demonstrated that re-irradiation induced significantly decreased testicular weights with a significant decrease in germ cell differentiation mRNA species (Spo11, Tnp1, Gfra1, Oct4, Sycp3, Ddx4, Boll, Crem, Prm1, and Acrosin). In the 13 Sertoli cell-specific mRNA species decreased upon irradiation, six mRNA species (Claudin-11, Espn, Fshr, GATA1, Inhbb, and Wt1) showed significant differences between single- and re-irradiation. At the same time, different decreases in Sertoli cell-specific mRNA species were found in single-irradiation (Aqp8, Clu, Cst12, and Wnt5a) and re-irradiation (Tjp1, occludin, ZO-1, and ZO-2) mice. These results indicate that long-term aspermatogenesis may differ after single- and re-irradiated treatment.

scholarly journals Profiling of infection specific mRNA transcripts of the European seabass Dicentrarchus labrax

BMC Genomics ◽  
2009 ◽  
Vol 10 (1) ◽  
pp. 157 ◽  
Author(s):  
Elena Sarropoulou ◽  
Pilar Sepulcre ◽  
Laura Poisa-Beiro ◽  
Victoriano Mulero ◽  
José Meseguer ◽  
...  
Keyword(s):  
Dicentrarchus Labrax ◽  
European Seabass ◽  
Mrna Transcripts ◽  
Specific Mrna

Profiling of maternal and developmental-stage specific mRNA transcripts in Atlantic halibut Hippoglossus hippoglossus

Gene ◽  
2007 ◽  
Vol 386 (1-2) ◽  
pp. 202-210 ◽  
Author(s):  
Jialin Bai ◽  
Christel Solberg ◽  
Jorge M.O. Fernandes ◽  
Ian A. Johnston
Keyword(s):  
Developmental Stage ◽  
Atlantic Halibut ◽  
Hippoglossus Hippoglossus ◽  
Mrna Transcripts ◽  
Specific Mrna

scholarly journals Glomerulus-specific mRNA transcripts and proteins identified through kidney expressed sequence tag database analysis

2007 ◽  
Vol 71 (9) ◽  
pp. 889-900 ◽  
Author(s):  
L. He ◽  
Y. Sun ◽  
J. Patrakka ◽  
P. Mostad ◽  
J. Norlin ◽  
...  
Keyword(s):  
Expressed Sequence Tag ◽  
Database Analysis ◽  
Mrna Transcripts ◽  
Expressed Sequence ◽  
Specific Mrna
Sign in / Sign up

Export Citation Format

Share Document