scholarly journals PCR amplification of SRY-related gene sequences reveals evolutionary conservation of the SRY-box motif.

1993 ◽  
Vol 2 (3) ◽  
pp. 218-222 ◽  
Author(s):  
A M Coriat ◽  
U Muller ◽  
J L Harry ◽  
D Uwanogho ◽  
P T Sharpe
Keyword(s):  
Pcr Amplification ◽  
Evolutionary Conservation ◽  
Related Gene ◽  
Gene Sequences

scholarly journals Differential effects of distamycin analogues on amplification of human gene sequences by polymerase-chain reaction

1995 ◽  
Vol 308 (2) ◽  
pp. 513-519 ◽  
Author(s):  
M Passadore ◽  
N Bianchi ◽  
G Feriotto ◽  
C Mischiati ◽  
P Giacomini ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pyrrole Ring ◽  
Human Gene ◽  
Pcr Amplification ◽  
Genomic Region ◽  
Ras Oncogene ◽  
Gene Sequences ◽  
Chain Reaction ◽  
Sequence Preference ◽  
Polymerase Chain

In this report we analyse the effects of distamycin and five distamycin analogues on amplification by polymerase-chain reaction (PCR) of two gene sequences displaying a different A+T/G+C content. The first was a 5′ region of the human oestrogen receptor (ER) gene, containing a (TA)26 stretch; the second was a CG-rich sequence of the human Ha-ras oncogene. The results obtained unequivocally demonstrate that the addition of one pyrrole ring significantly improves the ability of distamycin derivatives to interfere with PCR-mediated amplification of the human ER genomic region carrying a (TA)26 stretch. The distamycin analogues analysed differ in the number of pyrrole rings and in the presence of an N-formyl, an N-formimidoyl or a retroamide group at position X1. Among compounds carrying the same number of pyrrole rings, those carrying an N-formyl or an N-formimidoyl group retain a similar inhibitory activity. The retroamide analogues, on the contrary, are much less efficient in inhibiting PCR-mediated amplification of the 5′ER region. With respect to sequence selectivity both distamycin and distamycin analogues exhibit a sequence preference, since they do not inhibit PCR amplification of Ha-ras CG-rich gene regions, with the exception of a distamycin analogue carrying four pyrrole rings.

ras-Related gene sequences identified and isolated from Saccharomyces cerevisiae

Nature ◽  
1983 ◽  
Vol 306 (5944) ◽  
pp. 707-709 ◽  
Author(s):  
D. DeFeo-Jones ◽  
E. M. Scolnick ◽  
R. Koller ◽  
R. Dhar
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Related Gene ◽  
Gene Sequences

‘Candidatus Liberibacter americanus’, associated with citrus huanglongbing (greening disease) in São Paulo State, Brazil

2005 ◽  
Vol 55 (5) ◽  
pp. 1857-1862 ◽  
Author(s):  
Diva do Carmo Teixeira ◽  
Colette Saillard ◽  
Sandrine Eveillard ◽  
Jean Luc Danet ◽  
Paulo Inácio da Costa ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Pcr Amplification ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Intergenic Regions ◽  
The 16S Rrna Gene

Symptoms of huanglongbing (HLB) were reported in São Paulo State (SPS), Brazil, in March 2004. In Asia, HLB is caused by ‘Candidatus Liberibacter asiaticus' and in Africa by ‘Candidatus Liberibacter africanus’. Detection of the liberibacters is based on PCR amplification of their 16S rRNA gene with specific primers. Leaves with blotchy mottle symptoms characteristic of HLB were sampled in several farms of SPS and tested for the presence of liberibacters. ‘Ca. L. asiaticus' was detected in a small number of samples but most samples gave negative PCR results. Therefore, a new HLB pathogen was suspected. Evidence for an SPS-HLB bacterium in symptomatic leaves was obtained by PCR amplification with universal primers for prokaryotic 16S rRNA gene sequences. The amplified 16S rRNA gene was cloned and sequenced. Sequence analysis and phylogeny studies showed that the 16S rRNA gene possessed the oligonucleotide signatures and the secondary loop structure characteristic of the α-Proteobacteria, including the liberibacters. The 16S rRNA gene sequence phylogenetic tree showed that the SPS-HLB bacterium clustered within the α-Proteobacteria, the liberibacters being its closest relatives. For these reasons, the SPS-HLB bacterium is considered a member of the genus ‘Ca. Liberibacter’. However, while the 16S rRNA gene sequences of ‘Ca. L. asiaticus' and ‘Ca. L. africanus' had 98·4 % similarity, the 16S rRNA gene sequence of the SPS-HLB liberibacter had only 96·0 % similarity with the 16S rRNA gene sequences of ‘Ca. L. asiaticus' or ‘Ca. L. africanus’. This lower similarity was reflected in the phylogenetic tree, where the SPS-HLB liberibacter did not cluster within the ‘Ca. L asiaticus’/‘Ca. L. africanus group’, but as a separate branch. Within the genus ‘Candidatus Liberibacter’ and for a given species, the 16S/23S intergenic region does not vary greatly. The intergenic regions of three strains of ‘Ca. L. asiaticus’, from India, the People's Republic of China and Japan, were found to have identical or almost identical sequences. In contrast, the intergenic regions of the SPS-HLB liberibacter, ‘Ca. L. asiaticus' and ‘Ca. L. africanus' had quite different sequences, with similarity between 66·0 and 79·5 %. These results confirm that the SPS-HLB liberibacter is a novel species for which the name ‘Candidatus Liberibacter americanus' is proposed. Like the African and the Asian liberibacters, the ‘American’ liberibacter is restricted to the sieve tubes of the citrus host. The liberibacter could also be detected by PCR amplification of the 16S rRNA gene in Diaphorina citri, the psyllid vector of ‘Ca. L. asiaticus’, suggesting that this psyllid is also a vector of ‘Ca. L. americanus' in SPS. ‘Ca. L. americanus' was detected in 216 of 218 symptomatic leaf samples from 47 farms in 35 municipalities, while ‘Ca. L. asiaticus' was detected in only 4 of the 218 samples, indicating that ‘Ca. L. americanus' is the major cause of HLB in SPS.

scholarly journals Early onset of polyglandular failure is associated with HLA-DRB1*03.

2008 ◽  
Vol 159 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Manuela Dittmar ◽  
Maximilian Ide ◽  
Michael Wurm ◽  
George J Kahaly
Keyword(s):  
Mhc Class I ◽  
Fragment Size ◽  
Disease Onset ◽  
Pcr Amplification ◽  
Size Analysis ◽  
Healthy Controls ◽  
Related Gene ◽  
Early Disease ◽  
Immune Genes ◽  
Endocrine Diseases

ObjectivesPolyglandular failure or autoimmunity (PGA) involves at least two endocrine diseases. Several genes may play a role in its etiology. This study analyzed 1) whether HLA-DRB1, HLA-DQB1, and MHC class I chain-related gene A (MICA) polymorphisms are associated in PGA and 2) whether PGA patients display stronger associations with these immune genes than patients with monoglandular autoimmunity (MGA).DesignAssociation study.MethodsHLA-DRB1, HLA-DQB1, and MICA alleles were analyzed in 73 patients with PGA, 283 with MGA, and 206 healthy controls. The HLA-DRB1 and HLA-DQB1 polymorphisms were determined with PCR-amplified DNA being hybridized with PCR-sequence-specific oligonucleotide probes. MICA microsatellites were typed by PCR amplification and fragment size analysis on a DNA sequencer.ResultsHLA-DRB1*03 was strongly increased in patients with PGA (50.7%) versus both controls (21.8%, Pc<0.0001; RR=2.32, 95% CI=1.62–3.33) and MGA (11.4%, Pc<0.0001). HLA-DRB1*03 was highly prevalent in PGA patients with early versus late disease onset (P<0.05, logistic regression analysis). HLA-DRB1*04 allele carriers were more present in PGA versus controls (53.4% vs 22.4%, Pc<0.0001, RR=2.38, 95% CI=1.68–3.38). Further, HLA-DQB1*02 was increased in PGA versus controls (Pc<0.01), whereas HLA-DQB1*06 was decreased (Pc<0.001). Patients with PGA showed more MIC A5.1 and less MIC A6 allele carriers than controls (NS). Presence of the MIC A5.1 allele was not associated with the HLA-DRB1*03 or HLA-DQB1 alleles.ConclusionsHLA-DRB1*03 is a stronger genetic marker in PGA than in MGA, foremost in those with early disease onset.

scholarly journals DNA CTG triplet repeats involved in dynamic mutations of neurologically related gene sequences form stable duplexes

1995 ◽  
Vol 23 (21) ◽  
pp. 4303-4311 ◽  
Author(s):  
Kenneth G. Smith ◽  
Ji Jie ◽  
George E. Fox ◽  
Xiaolian Gao
Keyword(s):  
Related Gene ◽  
Triplet Repeats ◽  
Gene Sequences ◽  
Dynamic Mutations

scholarly journals First report of bovine anaemia associated Theileria sinensis infection and phylogenetic analyses of partial gene sequences of Theileria and Anaplasma species detected in naturally infected Malaysian cattle

2020 ◽  
Author(s):  
Onyinyechukwu Ada Agina ◽  
Mohd Rosly Shaari ◽  
Nur Mahiza Isa ◽  
Mokrish Ajat ◽  
Mohd Zamri-Saad ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Pcr Amplification ◽  
Trypanosoma Evansi ◽  
Control Measures ◽  
Rrna Gene ◽  
Infected Cattle ◽  
Anaplasma Platys ◽  
Gene Sequences ◽  
First Report

Abstract Background: Serious disease outbreaks in cattle are usually associated with blood pathogens. This study aims to detect blood pathogens namely Trypanosoma evansi, Theileria, Anaplasma and Mycoplasma species, and studied their phylogenetic relationships, haemato-biochemical abnormalities and erythrocyte osmotic fragility (EOF) in Malaysian cattle. Methods: Molecular analysis was achieved by PCR amplification and sequencing of PCR amplicons of 18SrRNA gene of Theileria species, 16SrRNA genes of Anaplasma and Mycoplasma species, 16SrRNA gene of Candidatus Mycoplasma haemobos, MPSP gene of T. orientalis and RoTaT1.2 VSG gene of Trypanosoma evansi, in sixty-one (61) Kedah-Kelantan X Brahman cattle from Pahang, Malaysia. Haemato-biochemical analyses were performed using automated analysers while EOF was determined with the aid of saline solutions. Results: PCR amplification produced the expected fragment sizes for MPSP gene of T. orientalis, msp4 gene of Anaplasma marginale, 16S rRNA gene of C. M. haemobos, RoTaT1.2VSG gene of T. evansi. Nucleotide blast demonstrated that sequences of the PCR amplicons showed a high degree of molecular similarity in comparison with reference sequences. Analysis of 18SrRNA gene sequences of Theileria species and 16S rRNA gene sequences of Anaplasma species revealed Theileria sinensis and Anaplasma platys as additional species detected in these cattle. Theileria species was the most detected blood pathogen in the sampled cattle. The blood picture of all cattle group revealed poikilocytosis, anisocytosis, rouleaux formation and degenerative left shift. Erythrocyte fragility values of all the cattle groups were above the reference range. Anaemia of the macrocytic normochromic type was observed in the Trypanosoma evansi; and Anaplasma platys + Theileria sinensis double species co-infected cattle. Normocytic normochromic anaemia was observed in the T. sinensis infected cattle group. Significant (p<0.05) increases in serum liver and kidney parameters, total protein, globulin, total and unconjugated bilirubin and decreased albumin was observed in the Trypanosoma evansi infected cattle.Conclusion: We present the first report of anaemia associated with Theileria sinensis infection in Malaysian cattle. Because of the high occurrence of bovine theileriosis and detection of Anaplasma platys, there is an urgent need for appropriate preventive and control measures, as Theileria species and A. platys are of great economic and zoonotic importance respectively.

scholarly journals Target Capture and Massive Sequencing of Genes Transcribed inMytilus galloprovincialis

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Umberto Rosani ◽  
Stefania Domeneghetti ◽  
Alberto Pallavicini ◽  
Paola Venier
Keyword(s):  
De Novo ◽  
454 Sequencing ◽  
Pcr Amplification ◽  
Gene Sequences ◽  
Target Capture ◽  
Reference Transcript ◽  
Mediterranean Mussel ◽  
Massive Sequencing ◽  
Genomic Regions ◽  
Next Generation Sequencing Ngs

Next generation sequencing (NGS) allows fast and massive production of both genome and transcriptome sequence datasets. As the genome of the Mediterranean musselMytilus galloprovincialisis not available at present, we have explored the possibility of reducing the whole genome sequencing efforts by using capture probes coupled with PCR amplification and high-throughput 454-sequencing to enrich selected genomic regions. The enrichment of DNA target sequences was validated by real-time PCR, whereas the efficacy of the applied strategy was evaluated by mapping the 454-output reads against reference transcript data already available forM. galloprovincialisand by measuring coverage, SNPs, number ofde novosequenced introns, and complete gene sequences. Focusing on a target size of nearly 1.5 Mbp, we obtained a target coverage which allowed the identification of more than 250 complete introns, 10,741 SNPs, and also complete gene sequences. This study confirms the transcriptome-based enrichment of gDNA regions as a good strategy to expand knowledge on specific subsets of genes also in nonmodel organisms.

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