scholarly journals The fission yeast meiosis-specific Dmc1 recombinase mediates formation and branch migration of Holliday junctions by preferentially promoting strand exchange in a direction opposite to that of Rad51

2011 ◽  
Vol 25 (5) ◽  
pp. 516-527 ◽  
Author(s):  
Y. Murayama ◽  
Y. Tsutsui ◽  
H. Iwasaki
Keyword(s):  
Fission Yeast ◽  
Holliday Junctions ◽  
Strand Exchange ◽  
Branch Migration ◽  
Yeast Meiosis ◽  
Direction Opposite

scholarly journals A mutation in helicase motif III ofE.coliRecG protein abolishes branch migration of Holliday junctions

1994 ◽  
Vol 22 (3) ◽  
pp. 308-313 ◽  
Author(s):  
Gary J. Sharples ◽  
Matthew C. Whitby ◽  
Lizanne Ryder ◽  
Robert G. Lloyd
Keyword(s):  
Holliday Junctions ◽  
Branch Migration

Rad54 protein promotes branch migration of Holliday junctions

Nature ◽  
2006 ◽  
Vol 442 (7102) ◽  
pp. 590-593 ◽  
Author(s):  
Dmitry V. Bugreev ◽  
Olga M. Mazina ◽  
Alexander V. Mazin
Keyword(s):  
Holliday Junctions ◽  
Branch Migration

scholarly journals Linear elements are stable structures along the chromosome axis in fission yeast meiosis

Chromosoma ◽  
2021 ◽  
Author(s):  
Da-Qiao Ding ◽  
Atsushi Matsuda ◽  
Kasumi Okamasa ◽  
Yasushi Hiraoka
Keyword(s):  
Fission Yeast ◽  
Strand Break ◽  
Wide Field ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Meiotic Chromosomes ◽  
Linear Elements ◽  
Yeast Meiosis ◽  
Chromosome Axis ◽  
Stable Structures

AbstractThe structure of chromosomes dramatically changes upon entering meiosis to ensure the successful progression of meiosis-specific events. During this process, a multilayer proteinaceous structure called a synaptonemal complex (SC) is formed in many eukaryotes. However, in the fission yeast Schizosaccharomyces pombe, linear elements (LinEs), which are structures related to axial elements of the SC, form on the meiotic cohesin-based chromosome axis. The structure of LinEs has been observed using silver-stained electron micrographs or in immunofluorescence-stained spread nuclei. However, the fine structure of LinEs and their dynamics in intact living cells remain to be elucidated. In this study, we performed live cell imaging with wide-field fluorescence microscopy as well as 3D structured illumination microscopy (3D-SIM) of the core components of LinEs (Rec10, Rec25, Rec27, Mug20) and a linE-binding protein Hop1. We found that LinEs form along the chromosome axis and elongate during meiotic prophase. 3D-SIM microscopy revealed that Rec10 localized to meiotic chromosomes in the absence of other LinE proteins, but shaped into LinEs only in the presence of all three other components, the Rec25, Rec27, and Mug20. Elongation of LinEs was impaired in double-strand break-defective rec12− cells. The structure of LinEs persisted after treatment with 1,6-hexanediol and showed slow fluorescence recovery from photobleaching. These results indicate that LinEs are stable structures resembling axial elements of the SC.

scholarly journals Immediate visualization of recombination events and chromosome segregation defects in fission yeast meiosis

Chromosoma ◽  
2019 ◽  
Vol 128 (3) ◽  
pp. 385-396 ◽  
Author(s):  
Dmitriy Li ◽  
Marianne Roca ◽  
Raif Yuecel ◽  
Alexander Lorenz
Keyword(s):  
Fission Yeast ◽  
Chromosome Segregation ◽  
Yeast Meiosis

Abstract 2112: CA-microsatellite instability as a factor of branch migration impeding in Holliday junctions

2012 ◽  
Author(s):  
Natalia Y. Karpechenko ◽  
Nina G. Dolinnaya ◽  
Vladimir I. Popenko ◽  
Marianna G. Yakubovskaya ◽  
Victoria K. Gasanova
Keyword(s):  
Microsatellite Instability ◽  
Holliday Junctions ◽  
Branch Migration
Sign in / Sign up

Export Citation Format

Share Document