scholarly journals DNA methylation regulates long-range gene silencing of an X-linked homeobox gene cluster in a lineage-specific manner

2006 ◽  
Vol 20 (24) ◽  
pp. 3382-3394 ◽  
Author(s):  
M. Oda ◽  
A. Yamagiwa ◽  
S. Yamamoto ◽  
T. Nakayama ◽  
A. Tsumura ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Gene Silencing ◽  
Gene Cluster ◽  
Long Range ◽  
Homeobox Gene ◽  
Specific Manner

scholarly journals The Rhox Homeobox Gene Cluster Is Imprinted and Selectively Targeted for Regulation by Histone H1 and DNA Methylation

2011 ◽  
Vol 31 (6) ◽  
pp. 1275-1287 ◽  
Author(s):  
J. A. MacLean ◽  
A. Bettegowda ◽  
B. J. Kim ◽  
C.-H. Lou ◽  
S.-M. Yang ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Gene Cluster ◽  
Homeobox Gene ◽  
Histone H1

scholarly journals The role of DNA methylation in ageing and cancer

2018 ◽  
Vol 77 (4) ◽  
pp. 412-422 ◽  
Author(s):  
A. E. Morgan ◽  
T. J. Davies ◽  
M. T. Mc Auley
Keyword(s):  
Dna Methylation ◽  
Gene Silencing ◽  
Methylation Status ◽  
Electrochemical Techniques ◽  
Regulation Of Gene Expression ◽  
Homeobox Gene ◽  
Gene Promoter ◽  
Disease States ◽  
Potential Biomarker ◽  
Aberrant Dna Methylation

The aim of the present review paper is to survey the literature related to DNA methylation, and its association with cancer and ageing. The review will outline the key factors, including diet, which modulate DNA methylation. Our rationale for conducting this review is that ageing and diseases, including cancer, are often accompanied by aberrant DNA methylation, a key epigenetic process, which is crucial to the regulation of gene expression. Significantly, it has been observed that with age and certain disease states, DNA methylation status can become disrupted. For instance, a broad array of cancers are associated with promoter-specific hypermethylation and concomitant gene silencing. This review highlights that hypermethylation, and gene silencing, of the EN1 gene promoter, a crucial homeobox gene, has been detected in various forms of cancer. This has led to this region being proposed as a potential biomarker for diseases such as cancer. We conclude the review by describing a recently developed novel electrochemical method that can be used to quantify the level of methylation within the EN1 promoter and emphasise the growing trend in the use of electrochemical techniques for the detection of aberrant DNA methylation.

scholarly journals Viroids as a Tool to Study RNA-Directed DNA Methylation in Plants

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1187
Author(s):  
Michael Wassenegger ◽  
Athanasios Dalakouras
Keyword(s):  
Dna Methylation ◽  
Rna Interference ◽  
Gene Silencing ◽  
Plant Cells ◽  
Transcriptional Gene Silencing ◽  
Rnai Machinery ◽  
Double Stranded Rna ◽  
Post Transcriptional Gene Silencing ◽  
Cumulative Amount

Viroids are plant pathogenic, circular, non-coding, single-stranded RNAs (ssRNAs). Members of the Pospiviroidae family replicate in the nucleus of plant cells through double-stranded RNA (dsRNA) intermediates, thus triggering the host’s RNA interference (RNAi) machinery. In plants, the two RNAi pillars are Post-Transcriptional Gene Silencing (PTGS) and RNA-directed DNA Methylation (RdDM), and the latter has the potential to trigger Transcriptional Gene Silencing (TGS). Over the last three decades, the employment of viroid-based systems has immensely contributed to our understanding of both of these RNAi facets. In this review, we highlight the role of Pospiviroidae in the discovery of RdDM, expound the gradual elucidation through the years of the diverse array of RdDM’s mechanistic details and propose a revised RdDM model based on the cumulative amount of evidence from viroid and non-viroid systems.

scholarly journals Syntaxin 1A Gene Is Negatively Regulated in a Cell/Tissue Specific Manner by YY1 Transcription Factor, Which Binds to the −183 to −137 Promoter Region Together with Gene Silencing Factors Including Histone Deacetylase

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 146
Author(s):  
Takahiro Nakayama ◽  
Toshiyuki Fukutomi ◽  
Yasuo Terao ◽  
Kimio Akagawa
Keyword(s):  
Transcription Factor ◽  
Gene Silencing ◽  
Protein Complex ◽  
Promoter Region ◽  
Neuronal Cell ◽  
Syntaxin 1A ◽  
Tissue Specific ◽  
Cell Tissue ◽  
Specific Manner ◽  
A Cell

The HPC-1/syntaxin 1A (Stx1a) gene, which is involved in synaptic transmission and neurodevelopmental disorders, is a TATA-less gene with several transcription start sites. It is activated by the binding of Sp1 and acetylated histone H3 to the −204 to +2 core promoter region (CPR) in neuronal cell/tissue. Furthermore, it is depressed by the association of class 1 histone deacetylases (HDACs) to Stx1a–CPR in non-neuronal cell/tissue. To further clarify the factors characterizing Stx1a gene silencing in non-neuronal cell/tissue not expressing Stx1a, we attempted to identify the promoter region forming DNA–protein complex only in non-neuronal cells. Electrophoresis mobility shift assays (EMSA) demonstrated that the −183 to −137 OL2 promoter region forms DNA–protein complex only in non-neuronal fetal rat skin keratinocyte (FRSK) cells which do not express Stx1a. Furthermore, the Yin-Yang 1 (YY1) transcription factor binds to the −183 to −137 promoter region of Stx1a in FRSK cells, as shown by competitive EMSA and supershift assay. Chromatin immunoprecipitation assay revealed that YY1 in vivo associates to Stx1a–CPR in cell/tissue not expressing Stx1a and that trichostatin A treatment in FRSK cells decreases the high-level association of YY1 to Stx1a-CPR in default. Reporter assay indicated that YY1 negatively regulates Stx1a transcription. Finally, mass spectrometry analysis showed that gene silencing factors, including HDAC1, associate onto the −183 to −137 promoter region together with YY1. The current study is the first to report that Stx1a transcription is negatively regulated in a cell/tissue-specific manner by YY1 transcription factor, which binds to the −183 to −137 promoter region together with gene silencing factors, including HDAC.

scholarly journals An Epigenetic Switch Involving Overlapping Fur and DNA Methylation Optimizes Expression of a Type VI Secretion Gene Cluster

PLoS Genetics ◽  
2011 ◽  
Vol 7 (7) ◽  
pp. e1002205 ◽  
Author(s):  
Yannick R. Brunet ◽  
Christophe S. Bernard ◽  
Marthe Gavioli ◽  
Roland Lloubès ◽  
Eric Cascales
Keyword(s):  
Dna Methylation ◽  
Gene Cluster ◽  
Epigenetic Switch ◽  
Type Vi Secretion

scholarly journals Gene‐orientation‐dependent DNA methylation patterns in the mouse prolactin ( Prl ) family gene cluster locus

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Koji Hayakawa ◽  
Naoko Hattori ◽  
Momo Nakanishi ◽  
Jun Ohgane ◽  
Satoshi Tanaka ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Gene Cluster ◽  
Gene Orientation ◽  
Family Gene ◽  
Methylation Patterns

scholarly journals Relationship between DNA Methylation States and Transcription of Individual Isoforms Encoded by the Protocadherin-α Gene Cluster

2008 ◽  
Vol 283 (18) ◽  
pp. 12064-12075 ◽  
Author(s):  
Masahumi Kawaguchi ◽  
Tomoko Toyama ◽  
Ryosuke Kaneko ◽  
Teruyoshi Hirayama ◽  
Yoshimi Kawamura ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Gene Cluster

scholarly journals The RHOX homeobox gene cluster is selectively expressed in human oocytes and male germ cells

2013 ◽  
Vol 28 (6) ◽  
pp. 1635-1646 ◽  
Author(s):  
H. W. Song ◽  
R. A. Anderson ◽  
R. A. Bayne ◽  
J. Gromoll ◽  
S. Shimasaki ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Germ Cells ◽  
Homeobox Gene ◽  
Male Germ Cells ◽  
Human Oocytes
Sign in / Sign up

Export Citation Format

Share Document