scholarly journals In Vivo and In Vitro Genetic Models of Congenital Heart Disease

2019 ◽  
pp. a036764 ◽  
Author(s):  
Uddalak Majumdar ◽  
Jun Yasuhara ◽  
Vidu Garg
Keyword(s):  
Congenital Heart Disease ◽  
Heart Disease ◽  
Congenital Heart ◽  
Genetic Models

scholarly journals Mending a broken heart: In vitro, in vivo and in silico models of congenital heart disease

2021 ◽  
Vol 14 (3) ◽  
pp. dmm047522
Author(s):  
Abdul Jalil Rufaihah ◽  
Ching Kit Chen ◽  
Choon Hwai Yap ◽  
Citra N. Z. Mattar
Keyword(s):  
Congenital Heart Disease ◽  
Heart Disease ◽  
Congenital Heart ◽  
Computational Models ◽  
Developing Heart ◽  
Heterogenous Group ◽  
In Silico Models ◽  
Large Animals

ABSTRACTBirth defects contribute to ∼0.3% of global infant mortality in the first month of life, and congenital heart disease (CHD) is the most common birth defect among newborns worldwide. Despite the significant impact on human health, most treatments available for this heterogenous group of disorders are palliative at best. For this reason, the complex process of cardiogenesis, governed by multiple interlinked and dose-dependent pathways, is well investigated. Tissue, animal and, more recently, computerized models of the developing heart have facilitated important discoveries that are helping us to understand the genetic, epigenetic and mechanobiological contributors to CHD aetiology. In this Review, we discuss the strengths and limitations of different models of normal and abnormal cardiogenesis, ranging from single-cell systems and 3D cardiac organoids, to small and large animals and organ-level computational models. These investigative tools have revealed a diversity of pathogenic mechanisms that contribute to CHD, including genetic pathways, epigenetic regulators and shear wall stresses, paving the way for new strategies for screening and non-surgical treatment of CHD. As we discuss in this Review, one of the most-valuable advances in recent years has been the creation of highly personalized platforms with which to study individual diseases in clinically relevant settings.

Usefulness of multidetector CT imaging to assess vascular stents in children with congenital heart disease: An in vivo and in vitro study

2008 ◽  
Vol 72 (4) ◽  
pp. 544-551 ◽  
Author(s):  
Joachim G. Eichhorn ◽  
Frederick R. Long ◽  
Claudia Jourdan ◽  
Johannes T. Heverhagen ◽  
Sharon L. Hill ◽  
...  
Keyword(s):  
Congenital Heart Disease ◽  
Heart Disease ◽  
Congenital Heart ◽  
Multidetector Ct ◽  
In Vitro Study ◽  
Ct Imaging ◽  
Vitro Study ◽  
Vascular Stents

scholarly journals Lactobacillus endocarditis with prosthetic material: a case report on non-surgical management with corresponding literature review

2014 ◽  
Vol 6 (3) ◽  
Author(s):  
Mena Botros ◽  
Deepa Mukundan
Keyword(s):  
Congenital Heart Disease ◽  
Heart Disease ◽  
Congenital Heart ◽  
Cyanotic Congenital Heart Disease ◽  
Prosthetic Material ◽  
Vancomycin Therapy ◽  
Accurate Identification ◽  
Gram Positive Bacteria ◽  
One Year

<em>Lactobacilli</em> are rod shaped gram positive bacteria that naturally colonize the human gastrointestinal and genitourinary tracts and occasionally cause disease in humans. <em>Lactobacillus</em> infections are found in patients who are immunocompromized or have severe comorbidities. We report <em>Lactobacillus</em> endocarditis in a 17-year-old adolescent girl with cardiac prosthetic material following surgical correction for complex cyanotic congenital heart disease. Accurate identification of the organism can be delayed. Despite <em>in vivo</em> susceptibility to vancomycin, our patient clinically failed vancomycin therapy but ultimately responded to a six-week course of penicillin, in addition to a 4-week course of clindamycin and gentamicin. She recovered without the need for surgical intervention and has been symptom free for one year. Upon review of the literature, we found that <em>Lactobacillus</em> endocarditis has not been reported in a pediatric patient with complex cyanotic congenital heart disease.

Patient-specific in vitro models for hemodynamic analysis of congenital heart disease – Additive manufacturing approach

2017 ◽  
Vol 54 ◽  
pp. 111-116 ◽  
Author(s):  
Rafael Medero ◽  
Sylvana García-Rodríguez ◽  
Christopher J. François ◽  
Alejandro Roldán-Alzate
Keyword(s):  
Congenital Heart Disease ◽  
Heart Disease ◽  
Additive Manufacturing ◽  
Congenital Heart ◽  
In Vitro Models ◽  
Patient Specific

OP35.07: In vitro fertilization does not predict fetal diagnosis of congenital heart disease

2011 ◽  
Vol 38 (S1) ◽  
pp. 157-157
Author(s):  
J. K. Votava-Smith ◽  
J. S. Glickstein ◽  
L. Simpson ◽  
I. A. Williams
Keyword(s):  
Congenital Heart Disease ◽  
Heart Disease ◽  
In Vitro Fertilization ◽  
Congenital Heart ◽  
Fetal Diagnosis ◽  
Vitro Fertilization

scholarly journals In Vivo Pressure-Radius Relationships of the Pulmonary Artery in Children with Congenital Heart Disease

Circulation ◽  
1971 ◽  
Vol 43 (4) ◽  
pp. 585-592 ◽  
Author(s):  
JAY M. M. JARMAKANI ◽  
THOMAS P. GRAHAM ◽  
D. WOODROW BENSON ◽  
RAMON V. CANENT ◽  
JOSEPH C. GREENFIELD
Keyword(s):  
Congenital Heart Disease ◽  
Heart Disease ◽  
Pulmonary Artery ◽  
Congenital Heart

scholarly journals GATA4 transgenic mice as an in vivo model of congenital heart disease

2015 ◽  
Vol 35 (6) ◽  
pp. 1545-1553 ◽  
Author(s):  
HUA HAN ◽  
YU CHEN ◽  
GANG LIU ◽  
ZENGQIANG HAN ◽  
ZHOU ZHAO ◽  
...  
Keyword(s):  
Congenital Heart Disease ◽  
Heart Disease ◽  
Transgenic Mice ◽  
Congenital Heart ◽  
In Vivo Model

scholarly journals The Real Need for Regenerative Medicine in the Future of Congenital Heart Disease Treatment

Biomedicines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 478
Author(s):  
Yuichi Matsuzaki ◽  
Matthew G. Wiet ◽  
Brian A. Boe ◽  
Toshiharu Shinoka
Keyword(s):  
Congenital Heart Disease ◽  
Heart Disease ◽  
Regenerative Medicine ◽  
Congenital Heart ◽  
Cardiovascular Surgery ◽  
Treatment Options ◽  
Growth Potential ◽  
Pulmonary Vein Stenosis ◽  
Pediatric Cardiovascular Surgery

Bioabsorbable materials made from polymeric compounds have been used in many fields of regenerative medicine to promote tissue regeneration. These materials replace autologous tissue and, due to their growth potential, make excellent substitutes for cardiovascular applications in the treatment of congenital heart disease. However, there remains a sizable gap between their theoretical advantages and actual clinical application within pediatric cardiovascular surgery. This review will focus on four areas of regenerative medicine in which bioabsorbable materials have the potential to alleviate the burden where current treatment options have been unable to within the field of pediatric cardiovascular surgery. These four areas include tissue-engineered pulmonary valves, tissue-engineered patches, regenerative medicine options for treatment of pulmonary vein stenosis and tissue-engineered vascular grafts. We will discuss the research and development of biocompatible materials reported to date, the evaluation of materials in vitro, and the results of studies that have progressed to clinical trials.

scholarly journals High throughput in vivo functional validation of candidate congenital heart disease genes in Drosophila

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Jun-yi Zhu ◽  
Yulong Fu ◽  
Margaret Nettleton ◽  
Adam Richman ◽  
Zhe Han
Keyword(s):  
Congenital Heart Disease ◽  
Heart Disease ◽  
High Throughput ◽  
Congenital Heart ◽  
De Novo ◽  
Disease Risk ◽  
In Vivo Model ◽  
Disease Genes ◽  
Specific Patient

Genomic sequencing has implicated large numbers of genes and de novo mutations as potential disease risk factors. A high throughput in vivo model system is needed to validate gene associations with pathology. We developed a Drosophila-based functional system to screen candidate disease genes identified from Congenital Heart Disease (CHD) patients. 134 genes were tested in the Drosophila heart using RNAi-based gene silencing. Quantitative analyses of multiple cardiac phenotypes demonstrated essential structural, functional, and developmental roles for more than 70 genes, including a subgroup encoding histone H3K4 modifying proteins. We also demonstrated the use of Drosophila to evaluate cardiac phenotypes resulting from specific, patient-derived alleles of candidate disease genes. We describe the first high throughput in vivo validation system to screen candidate disease genes identified from patients. This approach has the potential to facilitate development of precision medicine approaches for CHD and other diseases associated with genetic factors.

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