scholarly journals Different detection capabilities by mycological media for Candida isolates from mono- or dual-species cultures

2019 ◽  
Author(s):  
Giulia De Angelis ◽  
Giulia Menchinelli ◽  
Riccardo Torelli ◽  
Elena De Carolis ◽  
Patrizia Posteraro ◽  
...  
Keyword(s):  
Incubation Time ◽  
Laboratory Diagnosis ◽  
Correct Detection ◽  
Species Number ◽  
Detection Rates ◽  
Sabouraud Dextrose Agar ◽  
Detection Capabilities ◽  
Bromcresol Green

ABSTRACTObjectivesThe aim of this study was to compare the Candida bromcresol green (BCG) medium with the chromogenic (CHROM) Brilliance Candida agar and Sabouraud dextrose agar (SDA) media in regard to their capability of detecting Candida isolates from mono-or dual-species cultures.MethodsWe prepared Candida isolates’ suspensions to obtain mono-species (n = 18) or dual-species (n = 153) culture plates per each medium, and three readers independently observed 513 plates at 24-h, 48-h and 72-h incubation time. We scored reading results as correct, over or under detection compared to the expected species number(s).ResultsBCG showed significantly higher correct-detection and lower under-detection rates for all Candida species when observed by at least one reader. At 24-h reading, 12 mono-species cultures had correct (or over) detections in all media, whereas 106, 60 and 78 dual-species cultures had correct (or over) detections in BCG, CHROM or SDA, respectively.ConclusionBCG provides the basis for an accurate laboratory diagnosis of Candida infections.

Use of sodium hydroxide DNA extraction methods for nested PCR detection of Bretziella fagacearum in the sapwood of oak species in Minnesota

2021 ◽  
Author(s):  
Melanie Joy Moore ◽  
Jennifer Juzwik ◽  
Olga Saiapina ◽  
Snober Ahmed ◽  
Anna Yang ◽  
...  
Keyword(s):  
Sodium Hydroxide ◽  
Dna Extraction ◽  
Nested Pcr ◽  
Pathogen Detection ◽  
Abiotic Factors ◽  
Laboratory Diagnosis ◽  
Extraction Methods ◽  
White Oak ◽  
Detection Rates ◽  
Quercus Species

Oak wilt caused by Bretziella fagacearum is an important disease of Quercus species, but its diagnosis may be confused with damage resulting from other diseases, insects, or abiotic factors. Laboratory diagnosis is important in such situations and when disease control action is desired. Polymerase chain reaction (PCR) tests can provide accurate lab diagnosis within two days. Two variations of a simple DNA extraction protocol using sodium hydroxide (NaOH) were compared to that of the proprietary protocol of a commercially available kit (CK) for nested PCR to detect the pathogen in oak sapwood. High frequencies of pathogen detection (98 to 100% of 48 branch segments assayed) were found for northern pin oak using the two NaOH-based and the CK methods. Detection rates were similar but lower for bur oak (ranged from 58 to 79%) and white oak (ranged from 54 to 71%) regardless of DNA extraction method. Using our alternative DNA extraction protocols may reduce total time and cost of B. fagacearum detection in PCR-based diagnosis and other downstream applications.

scholarly journals Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?

2010 ◽  
Vol 58 (4) ◽  
pp. 281 ◽  
Author(s):  
Savitri Sharma ◽  
Sarita Kar ◽  
SrikantK Sahu ◽  
Bikash Samal ◽  
Aparajita Mallick ◽  
...  
Keyword(s):  
Laboratory Diagnosis ◽  
Fungal Keratitis ◽  
Sabouraud Dextrose Agar

scholarly journals Rapid diagnosis of Toxoplasma gondii using loop-mediated isothermal amplification assay in camels and small ruminants

2022 ◽  
Vol 11 (1) ◽  
Author(s):  
Gamil S. G. Zeedan ◽  
Abeer M. Abdalhamed ◽  
Raafat M. Shaapan ◽  
Amira H. El-Namaky
Keyword(s):  
Small Ruminants ◽  
Lamp Assay ◽  
Laboratory Diagnosis ◽  
Isothermal Amplification ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Blood Samples ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay ◽  
Detection Capabilities

Abstract Background This study was conducted to detect the presence of T. gondii in milk and blood samples using three different assays: enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), and loop-mediated isothermal amplification assay (LAMP). Whole blood, serum, and milk samples were collected from goats (n = 156), sheep (n = 261), and camels (n = 108) in different governorates in Egypt from December 2019 to February 2021 and screened by ELISA for anti-Toxoplasma IgG antibodies before DNA extraction. The target T. gondii DNA gene was detected and evaluated using the LAMP assay compared to PCR. Results T. gondii antibodies were found in milk and serum samples at the rates of (29.26%) and (36.58%) in camels, (34.18%) and (35.89%) in sheep, and (33.7%) and (36.36%) in goats, respectively. Similar to PCR, the percentages of LAMP tests for the detection of the T. gondii DNA gene in milk and blood samples of camels, sheep, and goats were (4.8, 14.63), (6.83, 7.69), and (7.79, 9.09), respectively. LAMP's sensitivity for detecting T. gondii in milk and blood samples, which was identical to that of PCR, was 100%. Conclusions The findings clearly demonstrated that there were no variations in T. gondii detection capabilities in milk and blood samples from various animals using both PCR and LAMP tests. It provides a quick, precise, and sensitive method of detecting T. gondii in a variety of samples that may be used both in the field and in laboratory diagnosis.

scholarly journals Evaluation of Dermatophyte Test Medium and Sabouraud Dextrose Agar for Isolation of Dermatophyte Species

BioMedica ◽  
2020 ◽  
Vol 36 (4) ◽  
pp. 362-366
Author(s):  
Dr. Majid Rauf Ahmad ◽  
Dr. Iffat Javed ◽  
Dr. Sohaila Mushtaq ◽  
Dr. Rubeena Hafeez ◽  
Dr. Kanwal Hassan Cheema
Keyword(s):  
Culture Media ◽  
Fungal Growth ◽  
Laboratory Diagnosis ◽  
Medical Institute ◽  
Test Medium ◽  
Isolation And Identification ◽  
Time Saving ◽  
Primary Isolation ◽  
Sabouraud Dextrose Agar ◽  
Dermatophyte Species

Background and Objective: Dermatophyte infections require laboratory diagnosis before treatment is started. Although direct microscopy is routinely performed but culture of dermatophytes is the gold standard. However, it takes about 4 weeks for species identification on primary media. Our aim was to compare dermatophyte test medium (DTM) as a screening medium for the isolation of dermatophytes in comparison with sabouraud dextrose agar (SDA). Methods: It was a comparative study carried out at the Department of Microbiology of Post Graduate Medical Institute, Lahore over a period of nine months. Samples were collected from one hundred patients with clinically suspected dermatophytoses after taking informed written consent. The samples were examined microscopically and then inoculated on two types of culture media, one Sabouraud dextrose agar (SDA) with added chloramphenicol, gentacin and cycloheximide and other dermatophyte test medium (DTM) with added chlortetracycline, gentacin and cyclohexamide. Results: Fungal growth was observed in fifty-six samples on culture. Out of the fifty-six positive on cultures, nineteen were that of dermatophytes. Out of n = 100 patients, ten were positive on SDA while n = 14 dermatophyte species were able to grow on DTM. A significantly higher positivity (P ³ 0.05) for isolating dermatophytes was observed by DTM as compared to SDA. DTM was able to isolate (71%) of the dermatophytes in first 10 days. Isolation rate of dermatophyte species was higher (73.68%) on DTM as compared to SDA which was 52.6%. Conclusion: Authors recommend the use of dermatophyte test medium for the primary isolation and identification of dermatophyte species to be more effective and time saving.

Laboratory diagnosis of thrombosis

1977 ◽  
Vol 137 (10) ◽  
pp. 1362-1364
Author(s):  
V. Gurevich
Keyword(s):  
Laboratory Diagnosis
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