scholarly journals A Spectral De-mixing Model for Triplex In Vivo Imaging of Optical Coherence Tomography Contrast Agents

2019 ◽  
Author(s):  
Edwin Yuan ◽  
Peng Si ◽  
Saba Shevidi ◽  
Adam de la Zerda
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agent ◽  
Contrast Agents ◽  
In Vivo Imaging ◽  
Spectroscopic Technique ◽  
Optical Coherence ◽  
Single Voxel ◽  
Gold Nanobipyramids

The ability to detect multiple contrast agents simultaneously would greatly enhance Optical Coherence Tomography (OCT) images, providing nuanced biological context to physiological structures. However, previous OCT contrast agent work has been limited to scenarios where only a single contrast agent could be robustly detected within each voxel. We present a novel spectroscopic technique for de-mixing the spectral signal from multiple OCT contrast agents within a single voxel. We validate our technique in vitro and also demonstrate in vivo imaging of three spectrally distinct gold nanobipyramids, trafficking within the lymphatic system of a live mouse. This approach opens the door to a much broader range of pre-clinical and clinical OCT applications where multiplexed labeling is desirable.

Gold Nanobipyramids as Second Near Infrared Optical Coherence Tomography Contrast Agents for in Vivo Multiplexing Studies

Nano Letters ◽  
2019 ◽  
Vol 20 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Peng Si ◽  
Saba Shevidi ◽  
Edwin Yuan ◽  
Ke Yuan ◽  
Ziv Lautman ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
Near Infrared ◽  
Optical Coherence ◽  
Gold Nanobipyramids

In vivo imaging of gold nanorod contrast agents using photothermal optical coherence tomography

2013 ◽  
Author(s):  
Jason M. Tucker-Schwartz ◽  
Travis A. Meyer ◽  
Chetan A. Patil ◽  
Craig L. Duvall ◽  
Melissa C. Skala
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
In Vivo Imaging ◽  
Gold Nanorod ◽  
Optical Coherence

scholarly journals A Spectral Demixing Model for Triplex In Vivo Imaging of Optical Coherence Tomography Contrast Agents

ACS Photonics ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 893-900
Author(s):  
Edwin Yuan ◽  
Peng Si ◽  
Yonatan Winetraub ◽  
Saba Shevidi ◽  
Adam de la Zerda
Keyword(s):  
Optical Coherence Tomography ◽  
Contrast Agents ◽  
In Vivo Imaging ◽  
Optical Coherence

scholarly journals Gold Nanobipyramids as Second Near Infrared Optical Coherence Tomography Contrast Agents for Multiplexed In Vivo Lymphangiography

2019 ◽  
Author(s):  
Peng Si ◽  
Saba Shevidi ◽  
Edwin Yuan ◽  
Ke Yuan ◽  
Ziv Lautman ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Lymph Node ◽  
Contrast Agents ◽  
Near Infrared ◽  
Lymphatic Drainage ◽  
Optical Coherence ◽  
Melanoma Tumor ◽  
Contrast Enhanced ◽  
Gold Nanobipyramids

AbstractDeveloping contrast-enhanced optical coherence tomography (OCT) techniques is important for specific imaging of tissue lesions, molecular imaging, cell-tracking, and highly sensitive microangiography and lymphangiography. Multiplexed OCT imaging in the second near infrared (NIR-II) window is highly desirable since it allows simultaneous imaging and tracking of multiple biological events in high resolution with deeper tissue penetration in vivo. Here we demonstrate that gold nanobipyramids can function as OCT multiplexing contrast agents, allowing the visualization of two separate lymphatic flows occurring simultaneously from different drainage basins into the same lymph node in a live mouse. Contrast-enhanced multiplexed lymphangiography of a melanoma tumor in vivo shows that the peritumoral lymphatic drainage upstream of the tumor is unidirectional, with some drainage directly into the tumor, but the lymphatic drainage from the tumor is multi-directional. We also demonstrate real-time tracking of the contrast agents draining from a melanoma tumor specifically to the sentinel lymph node of the tumor and the three-dimensional distribution of the contrast agents in the lymph node.

scholarly journals Optimization of X-ray Investigations in Dentistry Using Optical Coherence Tomography

Sensors ◽  
2021 ◽  
Vol 21 (13) ◽  
pp. 4554
Author(s):  
Ralph-Alexandru Erdelyi ◽  
Virgil-Florin Duma ◽  
Cosmin Sinescu ◽  
George Mihai Dobre ◽  
Adrian Bradu ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Radiation Dose ◽  
Imaging Techniques ◽  
Image Resolution ◽  
Optical Coherence ◽  
X Rays ◽  
High Quality ◽  
X Ray

The most common imaging technique for dental diagnoses and treatment monitoring is X-ray imaging, which evolved from the first intraoral radiographs to high-quality three-dimensional (3D) Cone Beam Computed Tomography (CBCT). Other imaging techniques have shown potential, such as Optical Coherence Tomography (OCT). We have recently reported on the boundaries of these two types of techniques, regarding. the dental fields where each one is more appropriate or where they should be both used. The aim of the present study is to explore the unique capabilities of the OCT technique to optimize X-ray units imaging (i.e., in terms of image resolution, radiation dose, or contrast). Two types of commercially available and widely used X-ray units are considered. To adjust their parameters, a protocol is developed to employ OCT images of dental conditions that are documented on high (i.e., less than 10 μm) resolution OCT images (both B-scans/cross sections and 3D reconstructions) but are hardly identified on the 200 to 75 μm resolution panoramic or CBCT radiographs. The optimized calibration of the X-ray unit includes choosing appropriate values for the anode voltage and current intensity of the X-ray tube, as well as the patient’s positioning, in order to reach the highest possible X-rays resolution at a radiation dose that is safe for the patient. The optimization protocol is developed in vitro on OCT images of extracted teeth and is further applied in vivo for each type of dental investigation. Optimized radiographic results are compared with un-optimized previously performed radiographs. Also, we show that OCT can permit a rigorous comparison between two (types of) X-ray units. In conclusion, high-quality dental images are possible using low radiation doses if an optimized protocol, developed using OCT, is applied for each type of dental investigation. Also, there are situations when the X-ray technology has drawbacks for dental diagnosis or treatment assessment. In such situations, OCT proves capable to provide qualitative images.

scholarly journals Study on Establishing Reference Safe Concentrations of MRI Contrast Agents for Optimized Images: Paramagnetic Gd-DTPA-BMEA and Superparamagnetic Ferucarbotran

2021 ◽  
Vol 11 (3) ◽  
pp. 1165
Author(s):  
Wen-Tien Hsiao ◽  
Yi-Hong Chou ◽  
Jhong-Wei Tu ◽  
Ai-Yih Wang ◽  
Lu-Han Lai
Keyword(s):  
Contrast Agent ◽  
Contrast Agents ◽  
Mri Contrast Agents ◽  
Mri Contrast Agent ◽  
In Vitro Experiments ◽  
Mri Contrast ◽  
In Vivo Experiments ◽  
Contrast Agent Injection

The purpose of this study is to establish the minimal injection doses of magnetic resonance imaging (MRI) contrast agents that can achieve optimized images while improving the safety of injectable MRI drugs. Gadolinium-diethylenetriamine penta-acetic acid (Gd-DTPA) and ferucarbotran, commonly used in clinical practice, were selected and evaluated with in vitro and in vivo experiments. MRI was acquired using T1-weighted (T1W) and T2-weighted (T2W) sequences, and the results were quantitatively analyzed. For in vitro experiments, results showed that T1W and T2W images were optimal when Gd-DTPA-bisamide (2-oxoethyl) (Gd-DTPA-BMEA) and ferucarbotran were diluted to a volume percentage of 0.6% and 0.05%; all comparisons were significant differences in grayscale statistics using one-way analysis of variance (ANOVA). For in vivo experiments, the contrast agent with optimal concentration percentages determined from in vitro experiments were injected into mice with an injection volume of 100 μL, and the images of brain, heart, liver, and mesentery before and after injection were compared. The statistical results showed that the p values of both T1W and T2W were less than 0.001, which were statistically significant. Under safety considerations for MRI contrast agent injection, optimized MRI images could still be obtained after reducing the injection concentration, which can provide a reference for the safety concentrations of MRI contrast agent injection in the future.

scholarly journals In Vivo Imaging and Morphometry of the Human Pre-Descemet's Layer and Endothelium With Ultrahigh-Resolution Optical Coherence Tomography

2016 ◽  
Vol 57 (6) ◽  
pp. 2782 ◽  
Author(s):  
Kostadinka Bizheva ◽  
Lacey Haines ◽  
Erik Mason ◽  
Benjamin MacLellan ◽  
Bingyao Tan ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
In Vivo Imaging ◽  
Optical Coherence ◽  
Ultrahigh Resolution

Swept source optical coherence tomography for in vivo imaging and vibrometry in the apex of the mouse cochlea

2015 ◽  
Author(s):  
Hee Yoon Lee ◽  
Patrick D. Raphael ◽  
Audrey K. Ellerbee ◽  
Brian E. Applegate ◽  
John S. Oghalai
Keyword(s):  
Optical Coherence Tomography ◽  
In Vivo Imaging ◽  
Optical Coherence ◽  
Swept Source
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