scholarly journals Purification and molecular characterization of phospholipase, antigen 5 and hyaluronidases from the venom of the Asian hornet (Vespa velutina)

2019 ◽  
Author(s):  
Rafael I. Monsalve ◽  
Ruth Gutiérrez ◽  
Ilka Hoof ◽  
Manuel Lombardero
Keyword(s):  
Study Data ◽  
Data Availability ◽  
Vespa Velutina ◽  
Allergen Components ◽  
Vespula Vulgaris ◽  
Proteomic Study ◽  
Complete Sequences ◽  
Dolichovespula Maculata ◽  
Antigen 5

AbstractThe aim of this study was to purify potential allergenic components of Vespa velutina venom, the yellow legged Asian Hornet, and perform a preliminary characterization of the purified proteins. Starting from the whole venom of V.velutina, several chromatographic steps allowed to purify the phospholipase (named Vesp v 1), as well as the antigen 5 (Vesp v 5, the only allergenic component described as such so far). The two hyaluronidase isoforms found (Vesp v 2A and Vesp v 2B) cannot be separated from each other, but they are partially purified and characterized. Purity of the isolated proteins in shown by SDSPAGE, as well as by the results of the N-terminal sequencing. This characterization and nLC-MS/MS data provide most of the sequence for Vesp v 1 and Vesp v 5 (72 and 84% coverage, respectively), confirming that the whole sequences of the isolated natural components match with the data available in public transcriptomic databases. It is of particular interest that Vesp v 1 is a glycosylated phospholipase, a fact that had only described so far for the corresponding allergen components of Dolichovespula maculata and Solenopsis invicta.The availability of the complete sequences of Vespa velutina components permits comparison with homologous sequences from other Hymenoptera. These data demonstrate the higher similarity among the species of the genera Vespa and Vespula, in comparison to Polistes species, as it is especially observed with the hyaluronidases isoforms: the isoform Vesp v 2A only exists in the former genera, and not in Polistes; in addition, the most abundant isoform (Vesp v 2B) exhibits 93% sequence identity with the Ves v 2 isoform of Vespula vulgaris.Finally, the isolated components might be useful for improving the diagnosis of patients that could be allergic to stings of this invasive Asian hornet, as it has been the case of an improved diagnosis and treatment of other Hymenoptera-sensitized patients.Data Availability StatementProteomic study data are deposited and made available publicly when data are published with the ProteomeExchange identifier PXD015381 in the Mass spectrometry Interactive Virtual Environment (MassIVE).Allergen nomenclature for Vesp v 5 (and also for any future demonstrated allergen from Vespa velutina) has been approved by the WHO/IUIS Committee (www.allergen.org); when this manuscript is accepted, the WHO/IUIS Committee will be informed so that the complete sequences included in this manuscript are also included in their database (with links to the UNIPROT database, who will also be informed that the data can be made public).

Characterization of the human T cell response to antigen 5 from Vespula vulgaris (Ves v 5)

2005 ◽  
Vol 35 (3) ◽  
pp. 367-373 ◽  
Author(s):  
B. Bohle ◽  
B. Zwolfer ◽  
G. F. Fischer ◽  
U. Seppala ◽  
T. Kinaciyan ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Response ◽  
T Cell Response ◽  
Vespula Vulgaris ◽  
Human T Cell ◽  
Ves V 5 ◽  
Antigen 5

scholarly journals Purification and molecular characterization of phospholipase, antigen 5 and hyaluronidases from the venom of the Asian hornet (Vespa velutina)

PLoS ONE ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. e0225672 ◽  
Author(s):  
Rafael I. Monsalve ◽  
Ruth Gutiérrez ◽  
Ilka Hoof ◽  
Manuel Lombardero
Keyword(s):  
Molecular Characterization ◽  
Vespa Velutina ◽  
Antigen 5

scholarly journals Characterization of the Complete Nucleotide Sequences of mcr-1-Encoding Plasmids From Enterobacterales Isolates in Retailed Raw Meat Products From the Czech Republic

2021 ◽  
Vol 11 ◽  
Author(s):  
Marketa Zelendova ◽  
Costas C. Papagiannitsis ◽  
Adam Valcek ◽  
Matej Medvecky ◽  
Ibrahim Bitar ◽  
...  
Keyword(s):  
Meat Products ◽  
Study Data ◽  
Sequence Type ◽  
Sequence Database ◽  
The Czech Republic ◽  
Raw Meat ◽  
Geographical Regions ◽  
Antimicrobial Resistance Genes ◽  
Retail Meat

The aim of our study was to determine complete nucleotide sequence of mcr-1-carrying plasmids from Enterobacterales isolates recovered from domestic and imported raw retailed meat and compare them with plasmids available at the GenBank sequence database. A set of 16 plasmids originating from Escherichia coli (n = 13), Klebsiella pneumoniae (n = 2), and Citrobacter braakii (n = 1) were analyzed. In our previous study, data from whole genome sequencing showed that mcr-1 gene was located on plasmids of different incompatibility groups (IncHI2, IncI2, and IncX4). The IncI2 (n = 3) and IncX4 (n = 8) plasmids harbored mcr-1.1 gene only, whereas IncHI2 sequence type 4 plasmids (n = 5) carried large multidrug resistance (MDR) regions. MDR regions of IncHI2 plasmids included additional antimicrobial resistance genes conferring resistance to β-lactams (blaTEM−1), aminoglycosides [aadA1, aadA2, and aph(6)-Id], macrolides [mef (B)], tetracycline (tetA, tetR), and sulphonamides (sul1, sul2, and sul3). Likewise, IncHI2 plasmids carried several insertion sequences including IS1, IS3, IS26, IS1326, and ISApl1. In conclusion, our findings confirmed the involvement of IncX4, IncI2, and IncHI2 plasmids in the dissemination of mcr-1.1 gene in several environmental niches, as in samples of retail meat originating from different geographical regions. In contrast to IncX4 and IncI2, IncHI2 plasmids were more diverse and carried additional genes for resistance to heavy metals and multiple antimicrobials.

scholarly journals Molecular Characterization of the FCoV-like Canine Coronavirus HLJ-071 in China

2021 ◽  
Author(s):  
Zhige Tian ◽  
Miaomiao Zheng ◽  
Ying Deng ◽  
Dandan Gou ◽  
Peng Guo ◽  
...  
Keyword(s):  
Variable Region ◽  
Open Reading Frames ◽  
Cell Tropism ◽  
Essential Information ◽  
Severe Diarrhea ◽  
S Gene ◽  
Canine Coronavirus ◽  
Complete Sequences ◽  
Feline Coronavirus

Abstract Background According to differences in antigens and genetic composition, canine coronavirus (CCoV) consists of two genotypes, CCoV-Ⅰ and CCoV-Ⅱ. Recently, CCoVs with mutant variations have been found to be pantropic and pathogenic in dogs. Results In this study, we isolated a CCoV, designated HLJ-071, from a dead 5-week-old female Welsh Corgi with severe diarrhea and vomiting. Sequence analysis suggested that HLJ-071 bears a complete ORF3abc when compared with classic CCoV isolates (1–71, K378 and S378). In addition, a variable region was located between the S gene and the open reading frames (ORF) 3a gene, in which HLJ-071 has a deletion of 104 nucleotides (nts) when compared with classic CCoV strains 1–71, S378 and K378. Phylogenetic analysis based on the S gene and complete sequences showed that HLJ-071 is closely related to Feline Coronavirus (FCoV) II. Recombination analysis suggested that HLJ-071 originated from the recombination of FCoV 79-1683, FCoV DF2 and CCoV A76. Finally, cell tropism experiments suggested that HLJ-071 is able to replicate in canine macrophages/monocytes. Conclusion The present study involved the isolation and genetic characterization of a variant CCoV strain. The spike protein and ORF3abc of CCoV might play a key role in viral tropism, which could affect replication in monocyte/macrophage cells. This will provide essential information for further understanding the evolution of CCoV in China.

Dynamic virtual infrastructure benchmarking: DynaVIBe

2010 ◽  
Vol 10 (4) ◽  
pp. 600-609 ◽  
Author(s):  
R. Sitzenfrei ◽  
S. Fach ◽  
M. Kleidorfer ◽  
C. Urich ◽  
W. Rauch
Keyword(s):  
Case Studies ◽  
Distribution Systems ◽  
Water Distribution ◽  
New Technologies ◽  
Study Data ◽  
Data Availability ◽  
Urban Structure ◽  
Real World Data ◽  
Virtual Infrastructure

In environmental engineering, identification of problems and their solutions as well as the identification of the relevant processes involved is often done by means of case study analyses. By researching the operation of urban drainage and water distribution systems, this methodology is suited to evaluate new technologies, strategies or measures with regard to their impact on the overall processes. However, data availability is often limited and data collection and the development of new models are both costly and time consuming. Hence, new technologies, strategies or measures can only be tested on a limited number of case studies. In several environmental disciplines a few virtual case studies have been manually developed to provide data for research tasks and these are repeatedly used in different research projects. Efforts have also been invested in tackling limited data availability with the algorithmic generation of virtual case studies having constant or varying boundary conditions. The data provided by such tools is nevertheless only available for a certain instance in time. With DynaVIBe (Dynamic Virtual Infrastructure Benchmarking), numerous virtual case studies are algorithmically generated with a temporal development of the urban structure (population and land use model) and infrastructure. This provides a methodology that allows for the analysis of future scenarios on a spatio-temporal city scale. By linking a population model with DynaVIBe's infrastructure models, socio-economics impacts on infrastructure and system coherences can be investigated. The problematic of limited case study data is solved by the algorithmic generation of an unlimited number of virtual case studies, which are dynamic over time. Additionally, this methodology can also be applied on real world data for probabilistic future scenario analysis.

Characterization of complete sequences of RNA 1 and RNA 2 of citrus variegation virus

2007 ◽  
Vol 153 (2) ◽  
pp. 385-388 ◽  
Author(s):  
W. Li ◽  
S. Adkins ◽  
M. E. Hilf
Keyword(s):  
Complete Sequences

scholarly journals Detection and Replication of Moku Virus in Honey Bees and Social Wasps

Viruses ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 607
Author(s):  
Andrea Highfield ◽  
Jessica Kevill ◽  
Gideon Mordecai ◽  
Jade Hunt ◽  
Summer Henderson ◽  
...  
Keyword(s):  
Honey Bee ◽  
Honey Bees ◽  
Social Wasp ◽  
Vespa Velutina ◽  
Vespa Crabro ◽  
Vespula Vulgaris ◽  
Virus Transfer ◽  
Ecological Importance ◽  
The Uk ◽  
Vespula Pensylvanica

Transmission of honey bee viruses to other insects, and vice versa, has previously been reported and the true ecological importance of this phenomenon is still being realized. Members of the family Vespidae interact with honey bees via predation or through the robbing of brood or honey from colonies, and these activities could result in virus transfer. In this study we screened Vespa velutina and Vespa crabro collected from Europe and China and also honey bees and Vespula vulgaris from the UK for Moku virus (MV), an Iflavirus first discovered in the predatory social wasp Vespula pensylvanica in Hawaii. MV was found in 71% of Vespula vulgaris screened and was also detected in UK Vespa crabro. Only seven percent of Vespa velutina individuals screened were MV-positive and these were exclusively samples from Jersey. Of 69 honey bee colonies screened, 43% tested positive for MV. MV replication was confirmed in Apis mellifera and Vespidae species, being most frequently detected in Vespula vulgaris. MV sequences from the UK were most similar to MV from Vespula pensylvanica compared to MV from Vespa velutina in Belgium. The implications of the transfer of viruses between the Vespidae and honey bees are discussed.

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