Expressions of Recombinant Venom Allergen, Antigen 5 of Yellowjacket (Vespula vulgaris) and Paper Wasp (Polistes annularis), in Bacteria or Yeast

1999 ◽  
Vol 16 (3) ◽  
pp. 410-416 ◽  
Author(s):  
Rafael I. Monsalve ◽  
Gang Lu ◽  
Te Piao King
Keyword(s):  
Paper Wasp ◽  
Vespula Vulgaris ◽  
Polistes Annularis ◽  
Antigen 5 ◽  
Venom Allergen

Expression of hornet genes encoding venom allergen antigen 5 in insects

1993 ◽  
Vol 22 (3-4) ◽  
pp. 303-313 ◽  
Author(s):  
Michael D. Tomalski ◽  
Te Piao King ◽  
Lois K. Miller
Keyword(s):  
Genes Encoding ◽  
Antigen 5 ◽  
Venom Allergen

scholarly journals Recombinant Allergens with Reduced Allergenicity but Retaining Immunogenicity of the Natural Allergens: Hybrids of Yellow Jacket and Paper Wasp Venom Allergen Antigen 5s

2001 ◽  
Vol 166 (10) ◽  
pp. 6057-6065 ◽  
Author(s):  
Te Piao King ◽  
Sui Y. Jim ◽  
Rafael I. Monsalve ◽  
Anne Kagey-Sobotka ◽  
Lawrence M. Lichtenstein ◽  
...  
Keyword(s):  
Paper Wasp ◽  
Recombinant Allergens ◽  
Wasp Venom ◽  
Yellow Jacket ◽  
Venom Allergen

scholarly journals cDNA cloning and primary structure of a white-face hornet venom allergen, antigen 5.

1988 ◽  
Vol 85 (3) ◽  
pp. 895-899 ◽  
Author(s):  
K. S. Fang ◽  
M. Vitale ◽  
P. Fehlner ◽  
T. P. King
Keyword(s):  
Cdna Cloning ◽  
Primary Structure ◽  
White Face ◽  
Antigen 5 ◽  
Venom Allergen

Characterization of the human T cell response to antigen 5 from Vespula vulgaris (Ves v 5)

2005 ◽  
Vol 35 (3) ◽  
pp. 367-373 ◽  
Author(s):  
B. Bohle ◽  
B. Zwolfer ◽  
G. F. Fischer ◽  
U. Seppala ◽  
T. Kinaciyan ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Response ◽  
T Cell Response ◽  
Vespula Vulgaris ◽  
Human T Cell ◽  
Ves V 5 ◽  
Antigen 5

scholarly journals Purification and molecular characterization of phospholipase, antigen 5 and hyaluronidases from the venom of the Asian hornet (Vespa velutina)

2019 ◽  
Author(s):  
Rafael I. Monsalve ◽  
Ruth Gutiérrez ◽  
Ilka Hoof ◽  
Manuel Lombardero
Keyword(s):  
Study Data ◽  
Data Availability ◽  
Vespa Velutina ◽  
Allergen Components ◽  
Vespula Vulgaris ◽  
Proteomic Study ◽  
Complete Sequences ◽  
Dolichovespula Maculata ◽  
Antigen 5

AbstractThe aim of this study was to purify potential allergenic components of Vespa velutina venom, the yellow legged Asian Hornet, and perform a preliminary characterization of the purified proteins. Starting from the whole venom of V.velutina, several chromatographic steps allowed to purify the phospholipase (named Vesp v 1), as well as the antigen 5 (Vesp v 5, the only allergenic component described as such so far). The two hyaluronidase isoforms found (Vesp v 2A and Vesp v 2B) cannot be separated from each other, but they are partially purified and characterized. Purity of the isolated proteins in shown by SDSPAGE, as well as by the results of the N-terminal sequencing. This characterization and nLC-MS/MS data provide most of the sequence for Vesp v 1 and Vesp v 5 (72 and 84% coverage, respectively), confirming that the whole sequences of the isolated natural components match with the data available in public transcriptomic databases. It is of particular interest that Vesp v 1 is a glycosylated phospholipase, a fact that had only described so far for the corresponding allergen components of Dolichovespula maculata and Solenopsis invicta.The availability of the complete sequences of Vespa velutina components permits comparison with homologous sequences from other Hymenoptera. These data demonstrate the higher similarity among the species of the genera Vespa and Vespula, in comparison to Polistes species, as it is especially observed with the hyaluronidases isoforms: the isoform Vesp v 2A only exists in the former genera, and not in Polistes; in addition, the most abundant isoform (Vesp v 2B) exhibits 93% sequence identity with the Ves v 2 isoform of Vespula vulgaris.Finally, the isolated components might be useful for improving the diagnosis of patients that could be allergic to stings of this invasive Asian hornet, as it has been the case of an improved diagnosis and treatment of other Hymenoptera-sensitized patients.Data Availability StatementProteomic study data are deposited and made available publicly when data are published with the ProteomeExchange identifier PXD015381 in the Mass spectrometry Interactive Virtual Environment (MassIVE).Allergen nomenclature for Vesp v 5 (and also for any future demonstrated allergen from Vespa velutina) has been approved by the WHO/IUIS Committee (www.allergen.org); when this manuscript is accepted, the WHO/IUIS Committee will be informed so that the complete sequences included in this manuscript are also included in their database (with links to the UNIPROT database, who will also be informed that the data can be made public).

Satellite Nests, Early Males, and Plasticity of Reproductive Behavior in a Paper Wasp

1989 ◽  
Vol 134 (5) ◽  
pp. 731-748 ◽  
Author(s):  
Robert E. Page, ◽  
David C. Post ◽  
Robert A. Metcalf
Keyword(s):  
Reproductive Behavior ◽  
Paper Wasp

scholarly journals Expansion and accelerated evolution of 9-exon odorant receptors in Polistes paper wasps

2021 ◽  
Author(s):  
Andrew W Legan ◽  
Christopher M Jernigan ◽  
Sara E Miller ◽  
Matthieu F Fuchs ◽  
Michael J Sheehan
Keyword(s):  
Gene Family ◽  
Odorant Receptor ◽  
Receptor Gene ◽  
Gene Tree ◽  
Gene Family Evolution ◽  
Paper Wasp ◽  
Gene Copy ◽  
Evolutionary Divergence ◽  
Wasp Species ◽  
Or Gene

Abstract Independent origins of sociality in bees and ants are associated with independent expansions of particular odorant receptor (OR) gene subfamilies. In ants, one clade within the OR gene family, the 9-exon subfamily, has dramatically expanded. These receptors detect cuticular hydrocarbons (CHCs), key social signaling molecules in insects. It is unclear to what extent 9-exon OR subfamily expansion is associated with the independent evolution of sociality across Hymenoptera, warranting studies of taxa with independently derived social behavior. Here we describe odorant receptor gene family evolution in the northern paper wasp, Polistes fuscatus, and compare it to four additional paper wasp species spanning ∼40 million years of evolutionary divergence. We find 200 putatively functional OR genes in P. fuscatus, matching predictions from neuroanatomy, and more than half of these are in the 9-exon subfamily. Most OR gene expansions are tandemly arrayed at orthologous loci in Polistes genomes, and microsynteny analysis shows species-specific gain and loss of 9-exon ORs within tandem arrays. There is evidence of episodic positive diversifying selection shaping ORs in expanded subfamilies. Values of omega (d  N/dS) are higher among 9-exon ORs compared to other OR subfamilies. Within the Polistes OR gene tree, branches in the 9-exon OR clade experience relaxed negative (purifying) selection relative to other branches in the tree. Patterns of OR evolution within Polistes are consistent with 9-exon OR function in CHC perception by combinatorial coding, with both natural selection and neutral drift contributing to interspecies differences in gene copy number and sequence.

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