scholarly journals Isolation and molecular identification of pectinase producing Aspergillus species from different soil samples of Bhubaneswar regions

2019 ◽  
Author(s):  
Sonali Satapathy ◽  
Pabitra Mohan Behera ◽  
Dhananjay Kumar Tanty ◽  
Shweta Srivastava ◽  
Hrudayanath Thatoi ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Industrial Development ◽  
Large Scale ◽  
Plant Biomass ◽  
Genetic Manipulation ◽  
Soil Samples ◽  
Industrial Wastes ◽  
Aspergillus Species ◽  
Computational Techniques ◽  
28S Rdna Sequence

AbstractWith the significant improvement of human civilization there is a spur in the urban, rural and industrial development, which has a profound effect on the surrounding natural environment. Increased utilization of natural resources is often associated with accumulation of waste materials whose management is crucial for sustainable development of life. Availability of different microorganisms in the soil facilitates the degradation of wastes through their potential enzymatic activities. Pectinase seems to be one of the important enzymes produced by a wide variety of microorganisms contained in the soil. It is mainly involved in maceration and rotting of plant extracts and debris by hydrolysis of 1,4-alpha glycosidic bonds of de-esterified pectate of plant call wall. In this paper we report molecular identification of some pectinase producing Aspergillus species selected from soil samples of five different zones of Bhubaneswar city using molecular biology and computational techniques. Among fifteen fungal isolates studied from these five zones Aspergillus parvisclerotigenus was potent for pectinase production next to Aspergillus niger in form of halozone of 0.6 mm. It’s 28S rDNA sequence also had some significant identity (>90%) with different subspecies of Aspergillus. We hope that our findings will helpful in genetic manipulation for improvement of fungal strains of isolates. Again large scale use of the improved Aspergillus strains can degrade plant biomass & diverse industrial wastes which will reduce environmental pollution of capital urban like Bhubaneswar.

scholarly journals Carbothermic Reduction of Zinc Containing Industrial Wastes: A Kinetic Model

2021 ◽  
Author(s):  
M. Leuchtenmueller ◽  
C. Legerer ◽  
U. Brandner ◽  
J. Antrekowitsch
Keyword(s):  
Mass Transfer ◽  
Zinc Oxide ◽  
Kinetic Model ◽  
Large Scale ◽  
Industrial Wastes ◽  
Oxide Reduction ◽  
Transfer Coefficients ◽  
Metallothermic Reduction ◽  
Metal Bath ◽  
Zinc Recovery

AbstractEffective recycling of zinc-containing industrial wastes, most importantly electric arc furnace dust, is of tremendous importance for the circular economy of the steel and zinc industry. Herein, we propose a comprehensive kinetic model of the combined carbothermic and metallothermic reduction of zinc oxide in a metal bath process. Pyro-metallurgical, large-scale lab experiments of a carbon-saturated iron melt as reduction agent for a molten zinc oxide slag were performed to determine reaction constants and accurately predict mass transfer coefficients of the proposed kinetic model. An experimentally determined kinetic model demonstrates that various reactions run simultaneously during the reduction of zinc oxide and iron oxide. For the investigated slag composition, the temperature-dependent contribution of the metallothermic zinc oxide reduction was between 25 and 50 pct of the overall reaction mechanism. The mass transfer coefficient of the zinc oxide reduction quadrupled from 1400 °C to 1500 °C. The zinc recovery rate was > 99.9 pct in all experiments.

scholarly journals Feasibility of Eco-Friendly Binary and Ternary Blended Binders Made of Fly-Ash and Oil-Refinery Spent Catalyst in Ready-Mixed Concrete Production

2018 ◽  
Vol 10 (9) ◽  
pp. 3136 ◽  
Author(s):  
Carla Costa ◽  
José Marques
Keyword(s):  
Large Scale ◽  
Construction Materials ◽  
International Standards ◽  
Oil Refinery ◽  
Industrial Wastes ◽  
Spent Catalyst ◽  
Concrete Production ◽  
Ready Mixed Concrete ◽  
Binary Blended Concrete ◽  
Mixed Concrete

Large-scale recycling of new industrial wastes or by-products in concrete has become a crucial issue for construction materials sustainability, with impact in the three pillars (environmental, social and economic), while still maintaining satisfactory, or improved, concrete performance. The main goal of the paper is to evaluate the technological feasibility of the partial, or total, replacement of fly-ashes (FA), widely used in ready-mixed concrete production, with spent equilibrium catalyst (ECat) from the oil-refinery industry. Three different concrete mixtures with binary binder blends of FA (33.3% by mass, used as reference) and of ECat (16.7% and 33.3%), as well as a concrete mixture with a ternary binder blend with FA and ECat (16.7%, of each) were tested regarding their mechanical properties and durability. Generically, in comparison with commercial concrete (i) 16.7% ECat binary blended concrete revealed improved mechanical strength and durability; (ii): ternary FA-ECat blended binder concrete presented similar properties; and (iii) 33% ECat binary blended concrete has a lower performance. The engineering performance of all ECat concretes meet both the international standards and the reference durability indicators available in the scientific literature. Thus, ECat can be a constant supply for ready-mixed eco-concretes production, promoting synergetic waste recycling across industries.

Biogas production from plant biomass used for phytoremediation of industrial wastes

2007 ◽  
Vol 98 (8) ◽  
pp. 1664-1669 ◽  
Author(s):  
V.K. Verma ◽  
Y.P. Singh ◽  
J.P.N. Rai
Keyword(s):  
Plant Biomass ◽  
Biogas Production ◽  
Industrial Wastes

scholarly journals Geographical Change of the Japanese National Land in the 200 Years Using INŌ’s Map with GIS

2019 ◽  
Vol 1 ◽  
pp. 1-2
Author(s):  
Yuji Murayama ◽  
Yuki Iwai
Keyword(s):  
Political System ◽  
Industrial Development ◽  
Large Scale ◽  
Geographic Information ◽  
Point Of View ◽  
The Political ◽  
Spatial Transformation ◽  
Survey Line ◽  
The Difference ◽  
The 19Th Century

<p><strong>Abstract.</strong> This presentation discusses the regional changes quantitatively in the 200 years through the overlay analysis of the present map and the INŌ’s map made by Tadataka INO in 1821 (Figure 1). INO surveyed the coastline and major roads on foot. He investigated not only survey lines, but also various geographic information such as rivers, lakes, mountains, village names, castles, temples, administrative boundaries, etc. Visualizing all of the 214 sheets of the INŌ’s large-scale map with Geographic Information Systems (GIS), we can analyse the national land condition seamlessly at the end of the Edo era.</p><p>Methodological point of view, we have serious problems including the scale, projection, identification of geographic features and so on, when we compare the old map with the present. In this connection, digitalizing the INŌ’s map as the GIS data is very useful to examine the spatial transformation scientifically during the 200 years. The digital INŌ’s map was constructed by employing the geo-reference function of GIS with the triangulation method. The survey line was converted into the line feature of vector data, and the place names were converted into the point feature of raster data. The distance of the survey line was measured by GIS-based geometric operation.</p><p>We obtained the following findings. The distributions of villages, ports, and facilities in western Japan were denser than those in eastern Japan in the 19th century. This was caused not only by the difference in natural environment and landform but also by socioeconomic factors including the locations of the castle towns and industrial activities. The regional structure has been dramatically transformed by the modernization of the political system, transportation system, and industrial development in reclaimed areas (Figure 2). It is concluded that most parts of changes in regional characteristics have been attained by overcoming the natural constraints. However, the difference in the political system has also been influential to the formation of the present regional system.</p>

scholarly journals Capitalismo distrettuale, localismi d'impresa, globalizzazione

2008 ◽  
Keyword(s):  
Industrial Development ◽  
Large Scale ◽  
International Markets ◽  
Scope Economies ◽  
North East ◽  
The North ◽  
Productive System ◽  
Dimensional Growth ◽  
It Revolution ◽  
Made In

From the late Sixties on, industrial development in Italy evolved through the spread of small and medium sized firms, aggregated in district networks, with an elevated propensity to enterprise and the marked presence of owner-families. Installed within the local systems, the industrial districts tended to simulate large-scale industry exploiting lower costs generated by factors that were not only economic. The districts are characterised in terms of territorial location (above all the thriving areas of the North-east and Centre) and sector, since they are concentrated in the "4 As" (clothing-fashion, home-decor, agri-foodstuffs, automation-mechanics), with some overlapping with "Made in Italy". How can this model be assessed? This is the crucial question in the debate on the condition and prospects of the Italian productive system between the supporters of its capacity to adapt and the critics of economic dwarfism. A dispassionate judgement suggests that the prospects of "small is beautiful" have been superseded, but that the "declinist" view, that sees only the dangers of globalisation and the IT revolution for our SMEs is risky. The concept of irreversible crisis that prevails at present is limiting, both because it is not easy either to "invent", or to copy, a model of industrialisation, and because there is space for a strategic repositioning of the district enterprises. The book develops considerations in this direction, showing how an evolution of the district model is possible, focusing on: gains in productivity, scope economies (through diversification and expansion of the range of products), flexibility of organisation, capacity to meld tradition and innovation aiming at product quality, dimensional growth of the enterprises, new forms of financing, active presence on the international markets and valorisation of the resources of the territory. It is hence necessary to reactivate the behavioural functions of the entrepreneurs.

scholarly journals Application of CRISPR/Cas9 Tools for Genome Editing in the White-Rot Fungus Dichomitus squalens

Biomolecules ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1526
Author(s):  
Joanna E. Kowalczyk ◽  
Shreya Saha ◽  
Miia R. Mäkelä
Keyword(s):  
Genome Editing ◽  
Plant Biomass ◽  
Genetic Manipulation ◽  
White Rot ◽  
White Rot Fungus ◽  
Detailed Knowledge ◽  
Wild Type ◽  
Low Frequencies ◽  
Dichomitus Squalens

Dichomitus squalens is an emerging reference species that can be used to investigate white-rot fungal plant biomass degradation, as it has flexible physiology to utilize different types of biomass as sources of carbon and energy. Recent comparative (post-) genomic studies on D. squalens resulted in an increasingly detailed knowledge of the genes and enzymes involved in the lignocellulose breakdown in this fungus and showed a complex transcriptional response in the presence of lignocellulose-derived compounds. To fully utilize this increasing amount of data, efficient and reliable genetic manipulation tools are needed, e.g., to characterize the function of certain proteins in vivo and facilitate the construction of strains with enhanced lignocellulolytic capabilities. However, precise genome alterations are often very difficult in wild-type basidiomycetes partially due to extremely low frequencies of homology directed recombination (HDR) and limited availability of selectable markers. To overcome these obstacles, we assessed various Cas9-single guide RNA (sgRNA) ribonucleoprotein (RNP) -based strategies for selectable homology and non-homologous end joining (NHEJ) -based gene editing in D. squalens. We also showed an induction of HDR-based genetic modifications by using single-stranded oligodeoxynucleotides (ssODNs) in a basidiomycete fungus for the first time. This paper provides directions for the application of targeted CRISPR/Cas9-based genome editing in D. squalens and other wild-type (basidiomycete) fungi.

scholarly journals Zebrafish Cancer Predisposition Models

2021 ◽  
Vol 9 ◽  
Author(s):  
Kim Kobar ◽  
Keon Collett ◽  
Sergey V. Prykhozhij ◽  
Jason N. Berman
Keyword(s):  
Large Scale ◽  
Genetic Manipulation ◽  
Bone Marrow Failure ◽  
Cancer Predisposition ◽  
Potential Candidate ◽  
Cancer Evolution ◽  
High Fecundity ◽  
Cancer Models ◽  
The Impact

Cancer predisposition syndromes are rare, typically monogenic disorders that result from germline mutations that increase the likelihood of developing cancer. Although these disorders are individually rare, resulting cancers collectively represent 5–10% of all malignancies. In addition to a greater incidence of cancer, affected individuals have an earlier tumor onset and are frequently subjected to long-term multi-modal cancer screening protocols for earlier detection and initiation of treatment. In vivo models are needed to better understand tumor-driving mechanisms, tailor patient screening approaches and develop targeted therapies to improve patient care and disease prognosis. The zebrafish (Danio rerio) has emerged as a robust model for cancer research due to its high fecundity, time- and cost-efficient genetic manipulation and real-time high-resolution imaging. Tumors developing in zebrafish cancer models are histologically and molecularly similar to their human counterparts, confirming the validity of these models. The zebrafish platform supports both large-scale random mutagenesis screens to identify potential candidate/modifier genes and recently optimized genome editing strategies. These techniques have greatly increased our ability to investigate the impact of certain mutations and how these lesions impact tumorigenesis and disease phenotype. These unique characteristics position the zebrafish as a powerful in vivo tool to model cancer predisposition syndromes and as such, several have already been created, including those recapitulating Li-Fraumeni syndrome, familial adenomatous polyposis, RASopathies, inherited bone marrow failure syndromes, and several other pathogenic mutations in cancer predisposition genes. In addition, the zebrafish platform supports medium- to high-throughput preclinical drug screening to identify compounds that may represent novel treatment paradigms or even prevent cancer evolution. This review will highlight and synthesize the findings from zebrafish cancer predisposition models created to date. We will discuss emerging trends in how these zebrafish cancer models can improve our understanding of the genetic mechanisms driving cancer predisposition and their potential to discover therapeutic and/or preventative compounds that change the natural history of disease for these vulnerable children, youth and adults.

scholarly journals Expression and manufacturing of protein therapeutics in spirulina

2021 ◽  
Author(s):  
Benjamin Jester ◽  
Hui Zhao ◽  
Mesfin Gewe ◽  
Thomas Adame ◽  
Lisa Perruzza ◽  
...  
Keyword(s):  
Large Scale ◽  
Genetic Manipulation ◽  
Low Cost ◽  
Downstream Processing ◽  
Arthrospira Platensis ◽  
Protein Therapeutics ◽  
High Productivity ◽  
Medical Needs ◽  
Manufacturing Platform ◽  
Safe Food

ABSTRACTArthrospira platensis (commonly known as spirulina) is a photosynthetic cyanobacterium1. It is a highly nutritious food that has been consumed for decades in the US, and even longer by indigenous cultures2. Its widespread use as a safe food source and proven scalability have driven frequent attempts to convert it into a biomanufacturing platform. But these were repeatedly frustrated by spirulina’s genetic intractability. We report here efficient and versatile genetic engineering methodology for spirulina that allows stable expression of bioactive protein therapeutics at high levels. We further describe large-scale, indoor cultivation and downstream processing methods appropriate for the manufacturing of biopharmaceuticals in spirulina. The potential of the platform is illustrated by pre-clinical development and human testing of an orally delivered antibody therapeutic against campylobacter, a major cause of infant mortality in the developing world and a growing antibiotic resistance threat3,4. This integrated development and manufacturing platform blends the safety of food-based biotechnology with the ease of genetic manipulation, rapid growth rates and high productivity characteristic of microbial platforms. These features combine for exceptionally low-cost production of biopharmaceuticals to address medical needs that are unfeasible with current biotechnology platforms.

Can we improve soil properties and plant biomass using rock powder as soil amendment?

2021 ◽  
Author(s):  
Lena Reifschneider ◽  
Vinzenz Franz Eichinger ◽  
Evelin Pihlap ◽  
Noelia Garcia-Franco ◽  
Anna Kühnel ◽  
...  
Keyword(s):  
Climate Change ◽  
Plant Growth ◽  
Large Scale ◽  
Plant Biomass ◽  
New Mineral ◽  
Severe Drought ◽  
Rock Powder ◽  
Overburden Material ◽  
Neutral Soil ◽  
Mitigate Climate Change

&lt;p&gt;The application of rock powder is an option to improve soil fertility while valorising the overburden material produced by industries. The &amp;#8220;enhanced weathering&amp;#8221; of silicate rock has also gained recent interest in the scientific community for its potential to mitigate climate change. However, the effect of rock powder on the soil physical properties remains unclear, especially under climate change (e.g., increasing drought events). Prior to any large scale application of rock powder, it is crucial to disentangle the potential effects of rock powder application on its environment. In a mesocosm experiment, we explored the effect of three rock powders on plant biomass, soil aggregation and organic carbon (OC) allocation within aggregates, in two soils with clayey and sandy textures, under regular watering or severe drought conditions. We demonstrate that the rock powder was the third factor after drought and soil texture significantly affecting the plant growth, resulting in a significant plant biomass decrease ranging from - 13 % to - 42 % compared with the control. We mainly attribute this effect to the increase of the already neutral soil pH, along with the release of excessive heavy metal amounts at a toxic range for the plant. Yet, we found that adding rock powder to the soil resulted in an increase of the relative amount of microaggregates in the soil by up to + 70 %, along with a re-distribution of OC within the fine fractions of the soil (up to + 32 % of OC in &lt; 250 &amp;#181;m fractions). The new mineral-mineral and organo-mineral interactions promoted by the rock powder addition could potentially favour OC persistence in soil on the long term. With our results, we insist on the potential risks for plant growth associated to the application of rock powder when not handled properly. In addition to the current enthusiasm around the capacity of rock powder to enhance carbon sequestration in the inorganic form, we also encourage scientists to focus their research on its effect on soil structure properties and OC storage.&lt;/p&gt;

Corporate Structure, Industrial Development, and Structural Change in South Africa

2021 ◽  
pp. 348-374
Author(s):  
Pamela Mondliwa ◽  
Simon Roberts
Keyword(s):  
South Africa ◽  
South African ◽  
Industrial Development ◽  
Large Scale ◽  
Economies Of Scale ◽  
Corporate Structure ◽  
Continuity And Change ◽  
Economic Concentration ◽  
And Control

The orientation of large corporations is at the heart of how countries develop. These firms make large-scale investments and realize economies of scale and scope, as well as make long-term commitments to the learning and research necessary to build capabilities required for industrial development. In many industries and sectors the large firms have key technologies, govern access to markets, and control material inputs which can shape the structure of an economy. The chapter reviews the changing corporate structure in South Africa focusing on the implications for industrial development, the evolving internationalization of South African businesses, and the political economy of economic policy. While the South African economy has remained highly concentrated, the corporate structure has altered in fundamental ways. The chapter identifies key elements of continuity and change to explain the implications of the continued high levels of economic concentration for the economy through the lens of the corporate structure.

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