scholarly journals Revisiting australian ectocarpus subulatus (phaeophyceae) from the hopkins river: distribution, abiotic environment, and associated microbiota

2019 ◽  
Author(s):  
Simon M. Dittami ◽  
Akira F. Peters ◽  
John West ◽  
Thierry Cariou ◽  
Hetty KleinJan ◽  
...  
Keyword(s):  
Brown Algae ◽  
Laboratory Culture ◽  
Bacterial Strains ◽  
Abiotic Environment ◽  
Low Salinity ◽  
Order Of Magnitude ◽  
Original Laboratory ◽  
Surrounding Areas

AbstractEctocarpus is a genus of common marine brown algae. In 1995 a strain of Ectocarpus was isolated from Hopkins River Falls, Victoria, Australia, constituting one of few available freshwater or nearly freshwater brown algae, and the only one belonging to Ectocarpus. It has since been used as a model to study acclimation and adaptation to low salinities and the role of its microbiota in these processes. However, little is known about the distribution of this strain or whether it represents a stable population. Furthermore, its microbiota may have been impacted by the long period of cultivation.Twenty-two years after the original finding we searched for Ectocarpus in the Hopkins River and surrounding areas. We found individuals with ITS and cox1 sequences identical to the original isolate at three sites upstream of Hopkins River Falls, but none at the original isolation site. The osmolarity of the water at these sites ranged from 74-170 mOsmol, and it was rich in sulfate. The diversity of bacteria associated with the algae in situ was approximately one order of magnitude higher than in previous studies of the original laboratory culture, and 95 alga-associated bacterial strains were isolated from E. subulatus filaments on site. In particular, Planctomycetes were abundant in situ but rare in the laboratory-cultured strain.Our results confirm that E. subulatus has stably colonized the Hopkins River, and the newly isolated algal and bacterial strains offer new possibilities to study the adaptation of Ectocarpus to low salinity and its interactions with its microbiome.

Low-Temperature Halo-Carbon Homoepitaxial Growth of 4H-SiC: Morphology, Doping, and Role of HCl Additive

2007 ◽  
Vol 556-557 ◽  
pp. 133-136 ◽  
Author(s):  
Huang De Lin ◽  
Galyna Melnychuk ◽  
Jeffery L. Wyatt ◽  
Yaroslav Koshka
Keyword(s):  
Low Temperature ◽  
Nitrogen Concentration ◽  
Aluminum Concentration ◽  
Silicon Cluster ◽  
Vapor Species ◽  
Aluminum Doping ◽  
Homoepitaxial Growth ◽  
Order Of Magnitude

Low-temperature epitaxial growth of 4H-SiC with CH3Cl carbon precursor was further developed. In-situ doping with nitrogen and aluminum was investigated. The nitrogen concentration in epitaxial layers grown on the C face was almost two orders of magnitude higher than that in the Si-face epilayers grown in the same growth run at 13000C. The opposite trend was observed for intentional aluminum doping, with more than an order of magnitude higher aluminum concentration incorporated in Si-face epilayers. High values of nitrogen and aluminum doping well in excess of 1020 cm-3 without any obvious epilayer morphology degradation can be achieved on C-face and Siface respectively. Addition of HCl during halo-carbon growth at 13000C resulted in drastic improvement of the surface morphology. Also, a significant increase of the growth rate took place confirming that the improvement in the epilayer morphology during HCl-assisted growth is predominantly related to silicon cluster etching by additional Cl-containing vapor species.

scholarly journals Tools for examining subglacial bed deformation

1992 ◽  
Vol 38 (130) ◽  
pp. 388-396 ◽  
Author(s):  
Erik Blake ◽  
Garry K. C. Clarke ◽  
Marc C. Gérin
Keyword(s):  
Strain Rate ◽  
In Situ Stress ◽  
Yukon Territory ◽  
Strain Rates ◽  
Strain Measurements ◽  
Bed Deformation ◽  
Instantaneous Strain ◽  
Order Of Magnitude

AbstractDeformation beneath soft-bedded glaciers may be a physical mechanism that contributes to flow instabilities such as surging. If the role of bed deformation is to be understood, a rheological description is required, but the development of a rheology is hampered by a lack of in situ stress and strain measurements. In this paper, we describe four techniques for measuring subglacial strain. Three of these give continuous strain measurements, a capability that permits calculation of instantaneous strain rates and allows comparison of strain data with other time series. To demonstrate the practicability of the techniques, sample results from three summers of experimentation beneath Trapridge Glacier. Yukon Territory, are presented. The data show that subglacial strain rate can vary in amplitude and polarity on an hourly time-scale,and that the instantaneous strain rate can exceed the mean strain rale by an order of magnitude. Observed negative strain rates suggest extrusive flow within basal sediments.

scholarly journals Tools for examining subglacial bed deformation

1992 ◽  
Vol 38 (130) ◽  
pp. 388-396 ◽  
Author(s):  
Erik Blake ◽  
Garry K. C. Clarke ◽  
Marc C. Gérin
Keyword(s):  
Strain Rate ◽  
In Situ Stress ◽  
Yukon Territory ◽  
Strain Rates ◽  
Strain Measurements ◽  
Bed Deformation ◽  
Instantaneous Strain ◽  
Order Of Magnitude

Abstract Deformation beneath soft-bedded glaciers may be a physical mechanism that contributes to flow instabilities such as surging. If the role of bed deformation is to be understood, a rheological description is required, but the development of a rheology is hampered by a lack of in situ stress and strain measurements. In this paper, we describe four techniques for measuring subglacial strain. Three of these give continuous strain measurements, a capability that permits calculation of instantaneous strain rates and allows comparison of strain data with other time series. To demonstrate the practicability of the techniques, sample results from three summers of experimentation beneath Trapridge Glacier. Yukon Territory, are presented. The data show that subglacial strain rate can vary in amplitude and polarity on an hourly time-scale,and that the instantaneous strain rate can exceed the mean strain rale by an order of magnitude. Observed negative strain rates suggest extrusive flow within basal sediments.

A molecularly defined cardiorespiratory interneuron expressing SDPFLRFamide/GDPFLRFamide in the snail Lymnaea: monosynaptic connections and pharmacology

1993 ◽  
Vol 69 (3) ◽  
pp. 915-927 ◽  
Author(s):  
D. R. Skingsley ◽  
K. Bright ◽  
N. Santama ◽  
J. van Minnen ◽  
M. J. Brierley ◽  
...  
Keyword(s):  
Full Range ◽  
Membrane Conductance ◽  
Excitatory Effect ◽  
Gene Encoding ◽  
Specific Expression ◽  
Giant Neuron ◽  
Order Of Magnitude ◽  
Synaptic Responses

1. Neuron-specific expression of alternately spliced exons of the gene encoding the Phe-Met-Arg-Phe-NH2 (FMRFamide) family of neuropeptides and the role of encoded peptides in synaptic transmission were examined in an identified cardiorespiratory interneuron, the visceral white interneuron (VWI), in the snail Lymnaea. 2. In situ hybridization using exon-specific probes showed VWI cytoplasmic expression of the exon encoding the Lymnaea heptapeptides Gly-Asp-Pro-Phe-Leu-Arg-Phe-NH2 (GDPFLRF amide) Ser-Asp-Pro-Phe-Leu-Arg-Phe-NH2 (SDPFLRF amide) but not the exon encoding the tetrapeptides FMRFamide and Phe-Leu-Arg-Phe-NH2 (FLRFamide). 3. The absence of the tetrapeptides (FMRFamide and FLRFamide) in the VWI was indirectly confirmed by the lack of immunoreactivity to a specific antibody raised against the sequence Leu-Tyr. This sequence is present in the Lymnaea tetrapeptide precursor, but not the heptapeptide precursor. 4. The VWI has monosynaptic connections with many identifiable neurons in the CNS. These were excitatory on three clusters of identified neurons [B group (Bgp), E group (Egp), and F group (Fgp)], inhibitory on another cluster [A group (Agp)] or biphasic (excitation followed by inhibition) on a single giant neuron [right pedal dorsal 1 (RPeD1)]. 5. The role of GDPFLRFamide/SDPFLRFamide as putative neurotransmitters was examined by comparing neuronally evoked postsynaptic responses with the effects of focal peptide application. 6. The heptapeptides closely mimicked the inhibitory responses (threshold pressure pipette concentration 10(-9) M) on the Agp cells and RPeD1, including an increase in membrane conductance. FMRFamide was 1 order of magnitude less potent. GDPFLRFamide/SDPFLRFamide, applied either alone or in “cocktails” (combinations of GDPFLRFamide, SDPFLRFamide, FMRFamide, and FLRFamide), did not reproduce the excitatory effect of the VWI on the Bgp, Egp, and Fgp cells. These peptides, applied either together or separately, inhibited the cells. 7. FMRFamide or FLRFamide, but not GDPFLRFamide or SDPFLRFamide, could reproduce the initial depolarizing component of the biphasic response on RPeD1. This only occurred at concentrations of > or = 10(-4) M (10(-3) M was necessary to get spikes on RPeD1) and may not be physiologically significant. 8. We conclude that at least one so far unidentified co-transmitter must be present in the VWI to account for its full range of synaptic responses.

scholarly journals Global methane and nitrous oxide emissions from non-marine waters

2021 ◽  
Author(s):  
Yajing Zheng ◽  
Shuang Wu ◽  
Shuqi Xiao ◽  
Kai Yu ◽  
Xiantao Fang ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
Dominant Role ◽  
Nitrous Oxide Emissions ◽  
Inland Waters ◽  
Natural Ecosystems ◽  
Marine Waters ◽  
Order Of Magnitude ◽  
Flux Component

Abstract Non-marine waters (i.e., rivers, reservoirs, lakes, ponds, streams and estuaries) are globally significant emitters of methane (CH4) and nitrous oxide (N2O) to the atmosphere, while global estimates of these emissions have been hampered due to the lack of a worldwide comprehensive database with the collection of complete CH4 and N2O flux components. Here we synthesize 2997 in-situ flux or concentration measurements of CH4 and N2O from 277 peer-reviewed publications to examine the role of non-marine waters in shaping climate change. Here we estimate that inland waters including rivers, reservoirs, lakes and streams together release 94.49 Tg CH4 yr− 1 (ebullition plus diffusion) and 1.52 Tg N2O yr− 1 (diffusion) to the atmosphere, yielding an overall CO2-equivalent emission total of 3.05 Pg CO2 yr− 1, representing roughly 59% of CO2 emissions (5.13 Pg CO2 yr− 1) from these four aquatic ecosystems, with lakes acting as the largest emitter for both trace gases. Ebullition is noticed as a dominant flux component, contributing up to 62–84% of total CH4 fluxes across all inland waters. Chamber-derived CH4 flux rates are significantly greater than those determined by diffusion model-based methods for commonly capturing of both diffusive and ebullitive fluxes. The synthesis of global N2O measurements projected that rivers exhibit the highest indirect N2O emission factor (EF5, 0.028%), while streams have the lowest EF5 value (0.015%). Our study reveals a major oversight in regional and global CH4 budgets from inland waters, caused by neglect of the dominant role of ebullition pathways in those emissions. The indirect EF5 values established in this study generally suggest an order of magnitude downward revision is required in current IPCC default EF5 values for inland waters and estuaries. Our findings further indicate that a comprehensive understanding of the magnitude and patterns of CH4 and N2O emissions from non-marine waters is essential in defining the way that these natural ecosystems shape our climate.

{10-12} Twinning Mechanism During In Situ Micro-Tensile Loading of Pure Mg: Role of Basal Slip and Twin-Twin Boundary Interaction

2020 ◽  
Author(s):  
Nicolò Maria della Ventura ◽  
Szilvia Kalácska ◽  
Daniele Casari ◽  
Thomas Edward James Edwards ◽  
Johann Michler ◽  
...  
Keyword(s):  
Twin Boundary ◽  
Basal Slip ◽  
Tensile Loading ◽  
Boundary Interaction

The role of (bio)surfactant sorption in promoting the bioavailability of nutrients localized at the solid-water interface

1999 ◽  
Vol 39 (7) ◽  
pp. 91-98 ◽  
Author(s):  
Ryan N. Jordan ◽  
Eric P. Nichols ◽  
Alfred B. Cunningham
Keyword(s):  
Mass Transfer ◽  
Cell Membrane ◽  
Substrate Concentration ◽  
Water Interface ◽  
Industrial Systems ◽  
Solid Liquid Interface ◽  
Solid Liquid ◽  
Surfactant Sorption

Bioavailability is herein defined as the accessibility of a substrate by a microorganism. Further, bioavailability is governed by (1) the substrate concentration that the cell membrane “sees,” (i.e., the “directly bioavailable” pool) as well as (2) the rate of mass transfer from potentially bioavailable (e.g., nonaqueous) phases to the directly bioavailable (e.g., aqueous) phase. Mechanisms by which sorbed (bio)surfactants influence these two processes are discussed. We propose the hypothesis that the sorption of (bio)surfactants at the solid-liquid interface is partially responsible for the increased bioavailability of surface-bound nutrients, and offer this as a basis for suggesting the development of engineered in-situ bioremediation technologies that take advantage of low (bio)surfactant concentrations. In addition, other industrial systems where bioavailability phenomena should be considered are addressed.

THE ROLE OF BACTERIAL COMMUNITIES ON IN-SITU ARSENIC AND IRON CYCLING IN RIVERBANKS ALONG THE MEGHNA RIVER, BANGLADESH

2020 ◽  
Author(s):  
Kimberly D. Myers ◽  
◽  
Katrina Lee Jewell ◽  
P.S.K. Knappett ◽  
Mehtaz M. Lipsi ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Iron Cycling
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