scholarly journals jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths

2019 ◽  
Author(s):  
Manuel Alexander Mohr ◽  
Daniel Bushey ◽  
Abhi Aggarwal ◽  
Jonathan S. Marvin ◽  
Emiliano Jimenez Marquez ◽  
...  
Keyword(s):  
Fiber Laser ◽  
Calcium Imaging ◽  
Femtosecond Lasers ◽  
State Of The Art ◽  
Two Photon ◽  
Photon Excitation ◽  
Calcium Indicator ◽  
Calcium Indicators ◽  
Photon Imaging ◽  
Two Photon Excitation

AbstractState-of-the-art GFP-based calcium indicators do not undergo efficient two-photon excitation at wavelengths above 1000 nm, for which inexpensive and powerful industrial femtosecond lasers are available. Here we report jYCaMP1, a yellow variant of jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1000 nm and enables improved two-color calcium imaging with RFP-based indicators.

In vivo label-free two-photon excitation autofluorescence microscopy of microvasculature using a 520 nm femtosecond fiber laser

2020 ◽  
Vol 45 (10) ◽  
pp. 2704
Author(s):  
Ting Wu ◽  
Jiuling Liao ◽  
Jia Yu ◽  
Yufeng Gao ◽  
Hui Li ◽  
...  
Keyword(s):  
Fiber Laser ◽  
Label Free ◽  
Two Photon ◽  
Photon Excitation ◽  
Femtosecond Fiber Laser ◽  
Two Photon Excitation

scholarly journals jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths

2020 ◽  
Vol 17 (7) ◽  
pp. 694-697 ◽  
Author(s):  
Manuel Alexander Mohr ◽  
Daniel Bushey ◽  
Abhi Aggarwal ◽  
Jonathan S. Marvin ◽  
Jeong Jun Kim ◽  
...  
Keyword(s):  
Fiber Laser ◽  
Two Photon ◽  
Calcium Indicator ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Coherent blue emission generated by Rb two-photon excitation using diode and femtosecond lasers

2017 ◽  
Vol 50 (8) ◽  
pp. 085001 ◽  
Author(s):  
Jesus P Lopez ◽  
Marco P Moreno ◽  
Marcio H G de Miranda ◽  
Sandra S Vianna
Keyword(s):  
Femtosecond Lasers ◽  
Blue Emission ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Gold Nanorod Enhanced Two-Photon Excitation Fluorescence of Photosensitizers for Two-Photon Imaging and Photodynamic Therapy

2014 ◽  
Vol 6 (4) ◽  
pp. 2700-2708 ◽  
Author(s):  
Tingting Zhao ◽  
Kuai Yu ◽  
Lin Li ◽  
Taishi Zhang ◽  
Zhenping Guan ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Gold Nanorod ◽  
Two Photon ◽  
Photon Excitation ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
Two Photon Excitation

scholarly journals Two-Color, Two-Photon Imaging at Long Excitation Wavelengths Using a Diamond Raman Laser

2016 ◽  
Vol 22 (4) ◽  
pp. 803-807 ◽  
Author(s):  
Johanna Trägårdh ◽  
Michelle Murtagh ◽  
Gillian Robb ◽  
Maddy Parsons ◽  
Jipeng Lin ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Laser System ◽  
Raman Laser ◽  
Alexa Fluor ◽  
Ti:Sapphire Laser ◽  
Two Photon ◽  
Photon Excitation ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
Two Photon Excitation

AbstractWe demonstrate that the second-Stokes output from a diamond Raman laser, pumped by a femtosecond Ti:Sapphire laser, can be used to efficiently excite red-emitting dyes by two-photon excitation at 1,080 nm and beyond. We image HeLa cells expressing red fluorescent protein, as well as dyes such as Texas Red and Mitotracker Red. We demonstrate the potential for simultaneous two-color, two-photon imaging with this laser by using the residual pump beam for excitation of a green-emitting dye. We demonstrate this for the combination of Alexa Fluor 488 and Alexa Fluor 568. Because the Raman laser extends the wavelength range of the Ti:Sapphire laser, resulting in a laser system tunable to 680–1,200 nm, it can be used for two-photon excitation of a large variety and combination of dyes.

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