scholarly journals Convergence of DNA methylation profiles in a novel environment in the reef coral Porites astreoides

2019 ◽  
Author(s):  
James L. Dimond ◽  
Steven B. Roberts

AbstractPhenotypic acclimatization is an organismal response to environmental change that may be rooted in epigenetic mechanisms. In reef building corals, organisms that are severely threatened by environmental change, some evidence suggests that DNA methylation is an environmentally responsive mediator of acclimatization. We investigated changes in DNA methylation of the reef coral Porites astreoides in response to simulated environmental change. Coral colonies were sampled from a variety of habitats on the Belize Barrier Reef and transplanted to a common garden for one year. We used restriction site associated DNA sequencing, including a methylation-sensitive variant, to subsample the genome and assess changes in DNA methylation levels after a year in the common garden. Methylation changes among the 629 CpG loci we recovered were subtle, yet coral methylomes were more similar to each other after a year in the common garden together, indicating convergence of methylation profiles in the common environment. Differentially methylated loci showed matches with both coding and non-coding RNA sequences with putative roles in intracellular signaling, apoptosis, gene regulation, and epigenetic crosstalk. There was a weak but positive and significant relationship between genetic and epigenetic variation, providing evidence of methylation heritability. Altogether, our results suggest that DNA methylation in P. astreoides is at least somewhat responsive to environmental change, reflective of the environment, and heritable, characteristics necessary for methylation to be implicated as part of potential transgenerational acclimatization responses.

2020 ◽  
Vol 62 ◽  
pp. 32-38
Author(s):  
E. A. Dolmatov ◽  
R. B. Borzayev ◽  
A. N. Shaipov

The results of the study of the duration of the juvenile period of indigenous Chechen willow leaf pear genotypes (Pyrus salicifolia Pall.) are given in connection with the acceleration of the breeding process and the use of selected forms in pear breeding for high precocity. The studies were carried out in 2016-2019 at OOO “Orchards of Chechnya” in accordance with the Agreement on creative cooperation with the Russian Research Institute of Fruit Crop Breeding. The work was carried out in accordance with generally accepted programs and methods. The objects of the study were one-year and two-year-old pear seedlings obtained from sowing seeds of selected dwarf and low-growing local Chechen forms of willow pear (P. salicifolia Pall.), laying fruit buds on annual growths and seedlings of Caucasian pear (P. caucasica Fed.), 20 500 pcs. of each specie. The aim of the research was to study the potential of precocity of willow pear seedlings and to reveal of selected forms with the greatest degree of this trait. Stratified seeds were sown in the sowing department of the OOO “Orchards of Chechnya” production nursery in April, 2017. The seedlings were grown according to the common technology in dryland conditions on the plot with chestnut soil. The first fl owering of plants was noted in the spring, 2019. As a result of the research, for the first time on a large number of the experimental material it was found that in the off spring of the indigenous Chechen willow leaf pear genotypes, the selection of a little more than 2% of seedlings with a very short juvenile period (2 years) was possible. They are of great interest in accelerating the breeding process and in the selection of new pear varieties with high precocity. 20 willow leaf pear genotypes were selected for the further use in breeding for high precocity and as sources of the trait of short juvenile period.


Author(s):  
Brian Silver ◽  
Irene Gulka ◽  
Michael Nicolle ◽  
Ramesh Sahjpaul ◽  
Vladimir Hachinski

Background:The observation of an intraluminal common carotid artery thrombus overlying a wall defect at ultrasonography or angiography is unusual. To our knowledge, there are no previous reports of a free-floating thrombus in the common carotid artery.Case Report:A 45-year-old woman who was previously healthy and on no medications presented with acute hemiparesis and aphasia. Following testing that included carotid duplex and trancranial Doppler ultrasonography, diffusion-weighted magnetic resonance imaging, and digital subtraction angiography, the patient underwent emergency open embolectomy. No underlying wall defect was seen at the time of imaging or surgery. No obvious hypercoagulable state could be identified. Her NIH Stroke Scale score improved from 26 at admission to 2 at three months and 1 at one year.Conclusions:Multimodal imaging may have improved diagnosis and management in this patient with a unique finding. The source of the thrombus remains obscure.


2013 ◽  
Vol 33 (1) ◽  
pp. 80-82
Author(s):  
Ujjowala Devi Shrestha

Children are primarily examined by paediatricians. In Nepal, child health is in low priority due to illiteracy, there is total lack of awareness about children’s eye health. The common avoidable causes of childhood blindness are refractive errors, amblyopia, retinopathy of prematurity (ROP), vitamin A deficiency (VAD), xerophthalmia, ophthalmia neonatorum (ON), congenital cataract, and retinoblastoma. Paediatricians could be the key persons for early referral of these children to a paediatric ophthalmologist. Paediatricians can send the patients for eye examination after birth within 6 weeks, at 6 months, at one year and before going to school. In conclusion, early screening and referral by the paediatricians to the paediatric ophthalmologist prevents children from being sightless. DOI: http://dx.doi.org/10.3126/jnps.v33i1.7605 J Nepal Paediatr Soc. 2013;33(1):80-82


Ecology ◽  
2015 ◽  
Vol 96 (5) ◽  
pp. 1411-1420 ◽  
Author(s):  
R. Cunning ◽  
N. Vaughan ◽  
P. Gillette ◽  
T. R. Capo ◽  
J. L. Maté ◽  
...  

2007 ◽  
Vol 61 (1-2) ◽  
pp. 53-64
Author(s):  
Svetlana Jeremic ◽  
Vladimir Radosavljevic

A disease in the koi carp (Cyprinus carpio koi) and the common carp (Cyprinus carpio carpio), caused by the herpesvirus and accompanied by a high mortality rate, has spread across numerous fish ponds all over the world since 1998, resulting in massive mortality and significant financial losses. The herpesvirus-like virus, called the koi herpesvirus (KHV) has been isolated and identified from the koi and the common carp in the course of the incidences of massive mortalities. The first appearance of a disease with a high mortality in the common and the koi carp caused by the koi herpesvirus (KHV) was described in 1998 in Israel and the United States of America (USA). Since that time, a large number of cases of outbreaks of this disease have been confirmed throughout the world, including the USA, Israel, and a large number of European countries. The deaths occurred seasonally, in late spring or early autumn, when the water temperature was from 18-28?C. The most important factor of the environment that affects the occurrence and gravity of this disease is the water temperature. This disease is currently considered one of the factors that present the biggest threat to populations of the common and the koi carp. Diseased fish are disoriented, their movements uncoordinated, their breathing rapid, gills swollen, and they have local skin lesions. The virus was isolated from tissue of diseased fish and cultivated on a KF-1 (koi fin cells) cell line. Electronic microscopy examinations revealed virus identical viral particles of the Herpesviridae family. Analyses of the virion polypeptide and DNA established differences between the KHV and the previously known herpesvirus of the Cyprinida family, Herpesvirus cyprini (CHV), and the virus of the channel catfish (Channel catfish virus - CCV). In the years 2004 and 2005, high mortality was established among one-year and two-year carp fry on three fish ponds. At two ponds, the deaths occurred among one year and two-year carp fry during the spring period, when the water temperature was over 18?C. During the autumn period, mortality was recorded among one-year carp fry at water temperatures above 23?C. On the grounds of the determined pathomorphological changes and the high mortality during the period of the year when the temperature was above 18?C, we suspect that KHV is also present in fish ponds in Serbia, even though the virus itself has not been isolated. The objective of this work is briefly to present the relevant data on this disease which is inflicting significant losses to carp production, to show the current distribution of this disease, the diagnostic methods, and the possibilities for the prevention and control of KHV.


PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5746 ◽  
Author(s):  
Verena Tams ◽  
Jennifer Lüneburg ◽  
Laura Seddar ◽  
Jan-Phillip Detampel ◽  
Mathilde Cordellier

Phenotypic plasticity is the ability of a genotype to produce different phenotypes depending on the environment. It has an influence on the adaptive potential to environmental change and the capability to adapt locally. Adaptation to environmental change happens at the population level, thereby contributing to genotypic and phenotypic variation within a species. Predation is an important ecological factor structuring communities and maintaining species diversity. Prey developed different strategies to reduce their vulnerability to predators by changing their behaviour, their morphology or their life history. Predator-induced life history responses inDaphniahave been investigated for decades, but intra-and inter-population variability was rarely addressed explicitly. We addressed this issue by conducting a common garden experiment with 24 clonal lines of EuropeanDaphnia galeataoriginating from four populations, each represented by six clonal lines. We recorded life history traits in the absence and presence of fish kairomones. Additionally, we looked at the shape of experimental individuals by conducting a geometric morphometric analysis, thus assessing predator-induced morphometric changes. Our data revealed high intraspecific phenotypic variation within and between fourD. galeatapopulations, the potential to locally adapt to a vertebrate predator regime as well as an effect of the fish kairomones on morphology ofD. galeata.


2018 ◽  
Vol 19 (7) ◽  
pp. 2144 ◽  
Author(s):  
Arthur Bartels ◽  
Qiang Han ◽  
Pooja Nair ◽  
Liam Stacey ◽  
Hannah Gaynier ◽  
...  

DNA methylation is an epigenetic modification required for transposable element (TE) silencing, genome stability, and genomic imprinting. Although DNA methylation has been intensively studied, the dynamic nature of methylation among different species has just begun to be understood. Here we summarize the recent progress in research on the wide variation of DNA methylation in different plants, organs, tissues, and cells; dynamic changes of methylation are also reported during plant growth and development as well as changes in response to environmental stresses. Overall DNA methylation is quite diverse among species, and it occurs in CG, CHG, and CHH (H = A, C, or T) contexts of genes and TEs in angiosperms. Moderately expressed genes are most likely methylated in gene bodies. Methylation levels decrease significantly just upstream of the transcription start site and around transcription termination sites; its levels in the promoter are inversely correlated with the expression of some genes in plants. Methylation can be altered by different environmental stimuli such as pathogens and abiotic stresses. It is likely that methylation existed in the common eukaryotic ancestor before fungi, plants and animals diverged during evolution. In summary, DNA methylation patterns in angiosperms are complex, dynamic, and an integral part of genome diversity after millions of years of evolution.


1992 ◽  
Vol 59 (1_suppl) ◽  
pp. 243-245
Author(s):  
F. Acanfora ◽  
P.G. Conti ◽  
D. Genesi ◽  
G. Morteo ◽  
F. Sereno ◽  
...  

We report the history of a patient with ureteroileocutaneous-stomy who developed stenosis of both ureteroileal anastomoses after one year from cystectomy for urothelial cancer. The strictures were managed by percutaneous dilatation with an angioplasty baioon catheter. The double J catheters were left in place across the stenosis for three months, then the left stent was removed. Afterwards the patient presented a fistula between the common iliac artery and the left ureter, and he underwent conservative surgery. We discuss the possible etiopathogenesis and the management of this rare pathology.


Author(s):  
Houjuan Xing ◽  
Chao Wang ◽  
Hongda Wu ◽  
Dechun Chen ◽  
Shu Li ◽  
...  

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