scholarly journals Evaluation of Trichoderma harzianum mutant lines in the resistance induction against white mold and growth promotion of common bean

2019 ◽  
Author(s):  
Renata Silva Brandão ◽  
Thiago Fernandes Qualhato ◽  
Paula Arielle Mendes Ribeiro Valdisser ◽  
Marcio Vinicius de C. B. Côrtes ◽  
Pabline Marinho Vieira ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Common Bean ◽  
Trichoderma Harzianum ◽  
Plant Disease Resistance ◽  
Specific Activity ◽  
Growth Promotion ◽  
Mutant Line ◽  
White Mold ◽  
Resistance Induction ◽  
Mutant Lines

ABSTRACTGenetic engineering has brought improvements of Trichoderma species for biotechnological processes such as agriculture. It has previously been reported that sm1 and aquaglyceroporin genes from Trichoderma harzianum are strongly expressed during pathogens biocontrol. We have previously shown that Sm1 plays a significant role in plant disease resistance and aquaporin (AQP) regulate physiological processes and responses related to water stress. Here, we report the evaluation of mutant lines with sm1 (deleated) and aqp (overexpressed) in Phaseoulus vulgaris growth promotion and disease resistance induction. It was investigated plants physiological and biochemical responses, plant growth promotion and biocontrol of Sclerotinia sclerotiorum, the causal agent of white mold. Treatments included Trichoderma harzianum wyld type, mutant line overexpressing aquaporin (Aqua), and deleted ΔEpl1mutant line. Our results demonstrated that Aqua mutant line increased leaf area in 25%, in comparison to non-treated plants. It also differed from other treatments, in comparison to plants with treatments ALL-42 and ΔEpl1, which increased 28% and 91%, respectively (Isso é abstract, ta confuse e extensor. Specific activity of β-1.3 glucanase was higher in plants treated with T. harzianum ΔEpl1 mutant isolate, in leaves and roots respectively with 2.07 Umg−1 and 2.57 Umg−1. Plants inoculated or not with S. sclerotiorum presented with 2.27 Umg-1 and 2.34 Umg-1 of β-1.3 glucanase on average, respectively, but enzymatic activity was higher on leaves when compared to the roots. The Aqua mutant demonstrated excellent growth promoting properties. Both mutants of T. harzianum do not induce resistance to white mold on common bean plants.

Disease-resistance related sequences in common bean

Genome ◽  
1999 ◽  
Vol 42 (1) ◽  
pp. 41-47 ◽  
Author(s):  
M I Rivkin ◽  
C E Vallejos ◽  
P E McClean
Keyword(s):  
Disease Resistance ◽  
Glycine Max ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Plant Disease Resistance ◽  
Nucleotide Binding ◽  
Related Sequence ◽  
Nucleotide Binding Sites ◽  
Population Comparison ◽  
Plant Disease Resistance Genes

Primers based on a conserved nucleotide binding site (NBS) found in several cloned plant disease resistance genes were used to amplify DNA fragments from the genome of common bean (Phaseolus vulgaris). Cloning and sequence analysis of these fragments uncovered eight unique classes of disease-resistance related sequences. All eight classes contained the conserved kinase 2 motif, and five classes contained the kinase 3a motif. Gene expression was noted for five of the eight classes of sequences. A clone from the SB3 class mapped 17.8 cM from the Ur-6 gene that confers resistance to several races of the bean rust pathogen Uromyces appendiculatus. Linkage mapping identified microclusters of disease-resistance related sequence in common bean, and sequences mapped to four linkage groups in one population. Comparison with similar sequences from soybean (Glycine max) revealed that any one class of common bean disease-resistance related sequences was more identical to a soybean NBS-containing sequence than to the sequence of another common bean class.Key words: nucleotide binding sites, disease-resistance related sequences, Phaseolus vulgaris, Glycine max.

scholarly journals Biocontrol of seed pathogens and growth promotion of common bean seedlings by Trichoderma harzianum

2011 ◽  
Vol 46 (8) ◽  
pp. 822-828 ◽  
Author(s):  
Daniel Diego Costa Carvalho ◽  
Sueli Corrêa Marques de Mello ◽  
Murillo Lobo Júnior ◽  
Alaerson Maia Geraldine
Keyword(s):  
Common Bean ◽  
Trichoderma Harzianum ◽  
Seed Treatment ◽  
Root Colonization ◽  
Growth Promotion ◽  
Bean Seed ◽  
Seed Health ◽  
Rhizosphere Competence ◽  
Absolute Control ◽  
Plant Length

The objective of this work was to evaluate isolates of Trichoderma harzianum regarding biocontrol of common bean seed-borne pathogens, plant growth promotion, and rhizosphere competence. Five isolates of T. harzianum were evaluated and compared with commercial isolate (Ecotrich), Carboxin+Thiram, and an absolute control. Bean seeds of the cultivar Jalo Precoce, contaminated with Aspergillus, Cladosporium, and Sclerotinia sclerotiorum, were microbiolized with antagonists, and seed health tests were carried out. Isolates were evaluated on autoclaved substrate and in field conditions. Ten days after sowing (DAS), plant length was measured. To test rhizosphere competence, isolates were applied in boxes containing autoclaved washed sand, and root colonization was evaluated at 10 DAS, using five plants per box. The most effective isolates in the seed health tests were: CEN287 and CEN289 to control Aspergillus; the commercial isolate to control Cladosporium; and CEN287 and CEN316 to control S. sclerotiorum. Isolates CEN289 and CEN290 promoted bean growth in greenhouse and field. Seed treatment with T. harzianum reduces the incidence of Aspergillus, Cladosporium, and S. sclerotiorum in 'Jalo Precoce' common bean seeds.

scholarly journals Nanofibrillation is an Effective Method to Produce Chitin Derivatives for Induction of Plant Responses in Soybean

Plants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 810
Author(s):  
Hironori Kaminaka ◽  
Chihiro Miura ◽  
Yukiko Isowa ◽  
Takaya Tominaga ◽  
Mamu Gonnami ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Growth Promotion ◽  
Expression Patterns ◽  
Plant Response ◽  
Plant Responses ◽  
Biological Functions ◽  
Resistance Induction ◽  
Chitin Nanofiber ◽  
Aerial Parts ◽  
Chitin Derivatives

Chitin, an N-acetylglucosamine polymer, is well-known to have unique biological functions, such as growth promotion and disease resistance induction in plants. Chitin has been expectedly used for improving crop yield using its functions; however, chitin derivatives, such as chitin oligosaccharide (CO) and chitosan, are widely used instead since chitin is difficult to handle because of its insolubility. Chitin nanofiber (CNF), produced from chitin through nanofibrillation, retains its polymeric structure and can be dispersed uniformly even in water. Here, the effects of CO and CNF on plant responses were directly compared in soybeans (Glycine max) to define the most effective method to produce chitin derivatives for plant response induction. The growth promotion of aerial parts was observed only in CNF-treated plants. The transcriptome analysis showed that the number of differentially expressed genes (DEGs) in CNF-treated soybeans was higher than in CO-treated soybeans. Notably, the expression patterns of DEGs were mostly similar but were strongly induced by CNF treatment as compared with the CO group. These results reveal that CNF can induce stronger plant response to chitin than CO in soybeans, suggesting nanofibrillation, rather than oligomerization, as a more effective method to produce chitin derivatives for plant response induction.

scholarly journals Biological control of white mold by Trichoderma harzianum in common bean under field conditions

2015 ◽  
Vol 50 (12) ◽  
pp. 1220-1224 ◽  
Author(s):  
Daniel Diego Costa Carvalho ◽  
Alaerson Maia Geraldine ◽  
Murillo Lobo Junior ◽  
Sueli Corrêa Marques de Mello
Keyword(s):  
Biological Control ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Sclerotinia Sclerotiorum ◽  
Trichoderma Harzianum ◽  
Experimental Period ◽  
Field Conditions ◽  
White Mold ◽  
Control Treatment

Abstract: The objective of this work was to evaluate Trichoderma harzianum isolates for biological control of white mold in common bean (Phaseolus vulgaris). Five isolates were evaluated for biocontrol of white mold in 'Perola' common bean under field conditions, in the 2009 and 2010 crop seasons. A commercial isolate (1306) and a control treatment were included. Foliar applications at 2x109 conidia mL-1 were performed at 42 and 52 days after sowing (DAS), in 2009, and at 52 DAS in 2010. The CEN287, CEN316, and 1306 isolates decreased the number of Sclerotinia sclerotiorum apothecia per square meter in comparison to the control, in both crop seasons. CEN287, CEN316, and 1306 decreased white mold severity during the experimental period, when compared to the control.

scholarly journals Metabolic and biofungicidal properties of maize rhizobacteria for growth promotion and plant disease resistance

2015 ◽  
Vol 14 (9) ◽  
pp. 811-819 ◽  
Author(s):  
A. Noumavo Pacome ◽  
A. Agbodjato Nadege ◽  
W. Gachomo Emma ◽  
A. Salami Hafiz ◽  
Baba-Moussa Farid ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Plant Disease Resistance ◽  
Plant Disease ◽  
Growth Promotion

White Mold–Resistant Interspecific Common Bean Germplasm Lines VCW 54 and VCW 55

2009 ◽  
Vol 3 (2) ◽  
pp. 191-197 ◽  
Author(s):  
Shree P. Singh ◽  
Henry Terán ◽  
Howard F. Schwartz ◽  
Kristen Otto ◽  
Margarita Lema
Keyword(s):  
Common Bean ◽  
White Mold
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