Disease-resistance related sequences in common bean

Genome ◽  
1999 ◽  
Vol 42 (1) ◽  
pp. 41-47 ◽  
Author(s):  
M I Rivkin ◽  
C E Vallejos ◽  
P E McClean
Keyword(s):  
Disease Resistance ◽  
Glycine Max ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Plant Disease Resistance ◽  
Nucleotide Binding ◽  
Related Sequence ◽  
Nucleotide Binding Sites ◽  
Population Comparison ◽  
Plant Disease Resistance Genes

Primers based on a conserved nucleotide binding site (NBS) found in several cloned plant disease resistance genes were used to amplify DNA fragments from the genome of common bean (Phaseolus vulgaris). Cloning and sequence analysis of these fragments uncovered eight unique classes of disease-resistance related sequences. All eight classes contained the conserved kinase 2 motif, and five classes contained the kinase 3a motif. Gene expression was noted for five of the eight classes of sequences. A clone from the SB3 class mapped 17.8 cM from the Ur-6 gene that confers resistance to several races of the bean rust pathogen Uromyces appendiculatus. Linkage mapping identified microclusters of disease-resistance related sequence in common bean, and sequences mapped to four linkage groups in one population. Comparison with similar sequences from soybean (Glycine max) revealed that any one class of common bean disease-resistance related sequences was more identical to a soybean NBS-containing sequence than to the sequence of another common bean class.Key words: nucleotide binding sites, disease-resistance related sequences, Phaseolus vulgaris, Glycine max.

scholarly journals Plant disease resistance genes encode members of an ancient and diverse protein family within the nucleotide-binding superfamily

1999 ◽  
Vol 20 (3) ◽  
pp. 317-332 ◽  
Author(s):  
Blake C. Meyers ◽  
Allan W. Dickerman ◽  
Richard W. Michelmore ◽  
Subramoniam Sivaramakrishnan ◽  
Bruno W. Sobral ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Resistance Genes ◽  
Plant Disease Resistance ◽  
Plant Disease ◽  
Nucleotide Binding ◽  
Protein Family ◽  
Disease Resistance Genes ◽  
Plant Disease Resistance Genes

scholarly journals Use of interracial hybridization in breeding the race Durango common bean

1993 ◽  
Vol 73 (3) ◽  
pp. 785-793 ◽  
Author(s):  
Shree P. Singh ◽  
Albeiro Molina ◽  
Carlos A. Urrea ◽  
J. Ariel Gutiérrez
Keyword(s):  
Disease Resistance ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Mosaic Virus ◽  
Seed Yield ◽  
Block Design ◽  
Bean Common Mosaic Virus ◽  
Mass Selection ◽  
Plant Seed ◽  
High Level

Recently, interracial hybridization was used successfully in breeding common bean (Phaseolus vulgaris L.), but its use has not been adequately documented. Approximately 125 lines with medium-sized seed were selected in the first cycle, mostly from race Durango × race Mesoamerica (both from the Middle American domestication center) single- and multiple-cross populations, for disease resistance and race Durango characteristics. Fifteen of these improved lines, three race Durango control cultivars, and one control cultivar each from races Jalisco and Mesoamerica were evaluated for 3 yr (1989–1991) at three locations in Colombia. A randomized complete block design with three replications was used. Lines were developed using visual mass selection for seed yield and/or resistance to diseases in F2 and F3, followed by single plant harvests in F4 or F5 and seed increases in F6 or F7. Lines resistant to bean common mosaic virus and possessing other desirable traits were yield-tested in F7 or F8. All but two lines outyielded Alteño and Flor de Mayo, the highest yielding control cultivars from races Durango and Jalisco, respectively. Two lines also outyielded Carioca, the race Mesoamerica control cultivar. Improved lines tended to possess higher yield per day. All lines were resistant to bean common mosaic virus and most lines also carried a high level of resistance to anthracnose. Plant, seed, and maturity characteristics of most improved lines were similar to those of race Durango control cultivars. These results support the use of interracial hybridization in improving race Durango common bean. Key words: Common bean, Phaseolus vulgaris, race Durango, interracial populations, seed yield, disease resistance

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