scholarly journals An Automated Addressable Microfluidics Device for Minimally Disruptive Manipulation of Cells and Fluids within Living Cultures

2019 ◽  
Author(s):  
Anh Tong ◽  
Quang Long Pham ◽  
Vatsal Shah ◽  
Akshay Naik ◽  
Paul Abatemarco ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Pore Space ◽  
Organ Transplant ◽  
Cell Types ◽  
Ex Situ ◽  
Proof Of Concept ◽  
Entire Duration ◽  
Multiple Cell ◽  
On Chip ◽  
The U.S

ABSTRACTAccording to the U.S. Department of Health & Human Services, nearly 115,000 people in the U.S needed a lifesaving organ transplant in 2018, while only ∼10% of them have received it. Yet, almost no artificial FDA-approved products are commercially available today – three decades after the inception of tissue engineering. It is hypothesized here that the major bottlenecks restricting its progress stem from lack of access to the inner pore space of the scaffolds. Specifically, the inability to deliver nutrients to, and clear waste from, the center of the scaffolds limits the size of the products that can be cultured. Likewise, the inability to monitor, and control, the cells after seeding them into the scaffold results in nonviable tissue, with an unacceptable product variability. To resolve these bottlenecks, we present a prototype addressable microfluidics device capable of minimally disruptive fluid and cell manipulations within living cultures. As proof-of-concept, we demonstrate its ability to perform additive manufacturing by seeding cells in spatial patterns (including co-culturing multiple cell types); and subtractive manufacturing by removing surface adherent cells via focused flow of trypsin. Additionally, we show that the device can sample fluids and perform cell “biopsies” (which can be subsequently sent for ex-situ analysis), from any location within its Culture Chamber. Finally, the on-chip plumbing is completely automated using external electronics. This opens the possibility to perform long-term computer-driven tissue engineering experiments, where the cell behavior is modulated in response to the minimally disruptive observations (e.g. fluid sampling and cell biopsies) throughout the entire duration of the cultures. It is expected that the proof-of-concept technology will eventually be scaled up to 3D addressable microfluidic scaffolds, capable of overcoming the limitations bottlenecking the transition of tissue engineering technologies to the clinical setting.

scholarly journals Highly versatile cell-penetrating peptide loaded scaffold for efficient and localised gene delivery to multiple cell types: From development to application in tissue engineering

Biomaterials ◽  
2019 ◽  
Vol 216 ◽  
pp. 119277 ◽  
Author(s):  
Rosanne M. Raftery ◽  
David P. Walsh ◽  
Lia Blokpoel Ferreras ◽  
Irene Mencía Castaño ◽  
Gang Chen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Gene Delivery ◽  
Cell Types ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
Multiple Cell

Tissue Engineering: Controlling Spatial Organization of Multiple Cell Types in Defined 3D Geometries (Adv. Mater. 41/2012)

2012 ◽  
Vol 24 (41) ◽  
pp. 5542-5542
Author(s):  
Halil Tekin ◽  
Jefferson G. Sanchez ◽  
Christian Landeros ◽  
Karen Dubbin ◽  
Robert Langer ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Spatial Organization ◽  
Cell Types ◽  
Multiple Cell

scholarly journals A high-throughput microfluidic bilayer co-culture platform to study endothelial-pericyte interactions

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Miles T. Rogers ◽  
Ashley L. Gard ◽  
Robert Gaibler ◽  
Thomas J. Mulhern ◽  
Rivka Strelnikov ◽  
...  
Keyword(s):  
High Throughput ◽  
Fluid Shear Stress ◽  
Cell Types ◽  
Fluid Shear ◽  
Multiple Cell ◽  
Cell Type Specific ◽  
On Chip ◽  
Tissue Models ◽  
Macromolecular Permeability

AbstractMicrophysiological organ-on-chip models offer the potential to improve the prediction of drug safety and efficacy through recapitulation of human physiological responses. The importance of including multiple cell types within tissue models has been well documented. However, the study of cell interactions in vitro can be limited by complexity of the tissue model and throughput of current culture systems. Here, we describe the development of a co-culture microvascular model and relevant assays in a high-throughput thermoplastic organ-on-chip platform, PREDICT96. The system consists of 96 arrayed bilayer microfluidic devices containing retinal microvascular endothelial cells and pericytes cultured on opposing sides of a microporous membrane. Compatibility of the PREDICT96 platform with a variety of quantifiable and scalable assays, including macromolecular permeability, image-based screening, Luminex, and qPCR, is demonstrated. In addition, the bilayer design of the devices allows for channel- or cell type-specific readouts, such as cytokine profiles and gene expression. The microvascular model was responsive to perturbations including barrier disruption, inflammatory stimulation, and fluid shear stress, and our results corroborated the improved robustness of co-culture over endothelial mono-cultures. We anticipate the PREDICT96 platform and adapted assays will be suitable for other complex tissues, including applications to disease models and drug discovery.

Layer-by-layer printing of cells and its application to tissue engineering

2004 ◽  
Vol 845 ◽  
Author(s):  
Priya Kesari ◽  
Tao Xu ◽  
Thomas Boland
Keyword(s):  
Tissue Engineering ◽  
Microelectromechanical Systems ◽  
Cell Types ◽  
Three Dimensions ◽  
Layer By Layer ◽  
Hematopoietic Stem ◽  
On Demand ◽  
Cell Structures ◽  
Aqueous Environments ◽  
Multiple Cell

ABSTRACTTissues and organs exhibit distinct shapes and functions nurtured by vascular connectivity. In order to mimic and examine these intricate structure-function relationships, it is necessary to develop efficient strategies for assembling tissue-like constructs. Many of the top-down fabrication techniques used to build microelectromechanical systems, including photolithography, are attractive due to the similar feature sizes, but are not suitable for delicate biological systems or aqueous environments. A layer-by layer approach has been proposed by us to pattern functional cell structures in three dimensions. Freeform cell structures are created by the inkjet method, in which cells are entrapped within hydrogels and crosslinked on demand. The cells are viable, functional and show potential for cell maturation as exemplified by the diversion of hematopoietic stem cells into multiple cell types. These results show promise for many tissue engineering applications.

scholarly journals A Paradigm Shift in Tissue Engineering: From a Top–Down to a Bottom–Up Strategy

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 935
Author(s):  
Theresa Schmidt ◽  
Yu Xiang ◽  
Xujin Bao ◽  
Tao Sun
Keyword(s):  
Tissue Engineering ◽  
Cell Types ◽  
Clinical Applications ◽  
Organ Shortage ◽  
Top Down ◽  
Bottom Up ◽  
Multiple Cell ◽  
Size And Morphology ◽  
Modular Scaffolds

Tissue engineering (TE) was initially designed to tackle clinical organ shortage problems. Although some engineered tissues have been successfully used for non-clinical applications, very few (e.g., reconstructed human skin) have been used for clinical purposes. As the current TE approach has not achieved much success regarding more broad and general clinical applications, organ shortage still remains a challenging issue. This very limited clinical application of TE can be attributed to the constraints in manufacturing fully functional tissues via the traditional top–down approach, where very limited cell types are seeded and cultured in scaffolds with equivalent sizes and morphologies as the target tissues. The newly proposed developmental engineering (DE) strategy towards the manufacture of fully functional tissues utilises a bottom–up approach to mimic developmental biology processes by implementing gradual tissue assembly alongside the growth of multiple cell types in modular scaffolds. This approach may overcome the constraints of the traditional top–down strategy as it can imitate in vivo-like tissue development processes. However, several essential issues must be considered, and more mechanistic insights of the fundamental, underpinning biological processes, such as cell–cell and cell–material interactions, are necessary. The aim of this review is to firstly introduce and compare the number of cell types, the size and morphology of the scaffolds, and the generic tissue reconstruction procedures utilised in the top–down and the bottom–up strategies; then, it will analyse their advantages, disadvantages, and challenges; and finally, it will briefly discuss the possible technologies that may overcome some of the inherent limitations of the bottom–up strategy.

scholarly journals Multicellular Co-Culture in Three-Dimensional Gelatin Methacryloyl Hydrogels for Liver Tissue Engineering

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1762 ◽  
Author(s):  
Juan Cui ◽  
Huaping Wang ◽  
Qing Shi ◽  
Tao Sun ◽  
Qiang Huang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Liver Function ◽  
Cell Viability ◽  
Liver Tissue ◽  
Three Dimensional ◽  
Microfluidic Channel ◽  
Cell Types ◽  
Liver Tissue Engineering ◽  
Multiple Cell

Three-dimensional (3D) tissue models replicating liver architectures and functions are increasingly being needed for regenerative medicine. However, traditional studies are focused on establishing 2D environments for hepatocytes culture since it is challenging to recreate biodegradable 3D tissue-like architecture at a micro scale by using hydrogels. In this paper, we utilized a gelatin methacryloyl (GelMA) hydrogel as a matrix to construct 3D lobule-like microtissues for co-culture of hepatocytes and fibroblasts. GelMA hydrogel with high cytocompatibility and high structural fidelity was determined to fabricate hepatocytes encapsulated micromodules with central radial-type hole by photo-crosslinking through a digital micromirror device (DMD)-based microfluidic channel. The cellular micromodules were assembled through non-contact pick-up strategy relying on local fluid-based micromanipulation. Then the assembled micromodules were coated with fibroblast-laden GelMA, subsequently irradiated by ultraviolet for integration of the 3D lobule-like microtissues encapsulating multiple cell types. With long-term co-culture, the 3D lobule-like microtissues encapsulating hepatocytes and fibroblasts maintained over 90% cell viability. The liver function of albumin secretion was enhanced for the co-cultured 3D microtissues compared to the 3D microtissues encapsulating only hepatocytes. Experimental results demonstrated that 3D lobule-like microtissues fabricated by GelMA hydrogels capable of multicellular co-culture with high cell viability and liver function, which have huge potential for liver tissue engineering and regenerative medicine applications.

Towards a human-on-chip: Culturing multiple cell types on a chip with compartmentalized microenvironments

Lab on a Chip ◽  
2009 ◽  
Vol 9 (22) ◽  
pp. 3185 ◽  
Author(s):  
Chi Zhang ◽  
Ziqing Zhao ◽  
Nur Aida Abdul Rahim ◽  
Danny van Noort ◽  
Hanry Yu
Keyword(s):  
Cell Types ◽  
Multiple Cell ◽  
On Chip

ADVANCED TISSUE ENGINEERING STRATEGIES FOR VASCULARIZED PARENCHYMAL CONSTRUCTS

2014 ◽  
Vol 14 (01) ◽  
pp. 1430001 ◽  
Author(s):  
JIANKANG HE ◽  
FENG XU ◽  
YAXIONG LIU ◽  
ZHONGMIN JIN ◽  
DICHEN LI
Keyword(s):  
Tissue Engineering ◽  
Cell Types ◽  
Great Promise ◽  
Top Down ◽  
Bottom Up ◽  
Organ Specific ◽  
Recent Developments ◽  
Multiple Cell ◽  
Complex Structural

The fabrication of vascularized parenchymal organs to alleviate donor shortage in organ transplantation is the holy grail of tissue engineering. However, conventional tissue-engineering strategies have encountered huge challenges in recapitulating complex structural organization of native organs (e.g., orderly arrangement of multiple cell types and vascular network), which plays an important role in engineering functional vascularized parenchymal constructs in vitro. Recent developments of various advanced tissue-engineering strategies have exhibited great promise in replicating organ-specific architectures into artificial constructs. Here, we review the recent advances in top-down and bottom-up strategies for the fabrication of vascularized parenchymal constructs. We highlight the fabrication of microfluidic scaffolds potential for nutrient transport or vascularization as well as the controlled multicellular arrangement. The advantages as well as the limitations associated with these strategies will be discussed. It is envisioned that the combination of microfluidic concept in top-down strategies and multicellular arrangement concept in bottom-up strategies could potentially generate new insights for the fabrication of vascularized parenchymal organs.

Biological Characteristics of Dental Stem Cells for Tissue Engineering

2013 ◽  
Vol 541 ◽  
pp. 51-59 ◽  
Author(s):  
G. Mori ◽  
G. Brunetti ◽  
A. Ballini ◽  
A. Di Benedetto ◽  
U. Tarantino ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adult Stem Cells ◽  
Cell Types ◽  
Biological Characteristics ◽  
Dental Stem Cells ◽  
Different Types ◽  
Multiple Cell ◽  
New Evidence ◽  
Effective Treatments

Scientists have recently focused their attention on adult stem cells as new and more effective treatments for different diseases and disabilities. In fact, it is known that stem cells are capable of renewing themselves and that they can generate multiple cell types. Today, there is new evidence that stem cells are present in far more tissues and organs than once thought and that these cells are capable of developing into more kinds of cells than previously imagined. In this chapter, we focus the attention on teeth as source of stem cells. In particular, we describe the characteristic of the different types of dental stem cells and their use in tissue engineering.

Sign in / Sign up

Export Citation Format

Share Document