scholarly journals Reversible Pulmonary Trunk Banding. XI: Myocardial Vascular Endothelial Growth Factor Expression in Young Goats Submitted to Ventricular Retraining

2019 ◽  
Author(s):  
Renato S. Assad ◽  
Eduardo A. V. Rocha ◽  
Vera D. Aiello ◽  
Tiago A. Meniconi ◽  
Maria C. D. Abduch ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Pressure Ratio ◽  
Study Groups ◽  
Aortic Pressure ◽  
Control Group ◽  
Vascular Endothelial ◽  
Vegf Expression ◽  
Before And After ◽  
Endothelial Growth Factor

ABSTRACTBackgroundVentricle retraining has been extensively studied by our laboratory. Previous studies have demonstrated that intermittent overload causes a more efficient ventricular hypertrophy. The adaptive mechanisms involved in the ventricle retraining are not completely established. This study assessed vascular endothelial growth factor (VEGF) expression in the ventricles of goats submitted to systolic overload.MethodsTwenty-one young goats were divided into 3 groups (7 animals each): control, 96-hour continuous systolic overload, and intermittent systolic overload (four 12-hour periods of systolic overload paired with 12-hour resting period). During the 96-hour protocol, systolic overload was adjusted to achieve a right ventricular (RV) / aortic pressure ratio of 0.7. Hemodynamic evaluations were performed daily before and after systolic overload. Echocardiograms were obtained preoperatively and at protocol end to measure cardiac masses thickness. At study end, the animals were killed for morphologic evaluation and immunohistochemical assessment of VEGF expression.ResultsRV-trained groups developed hypertrophy of RV and septal masses, confirmed by increased weight and thickness, as expected. In the study groups, there was a small but significantly increased water content of the RV and septum compared with those in the control group (p<0.002). VEGF expression in the RV myocardium was greater in the intermittent group (2.89% ± 0.41%) than in the continuous (1.80% ± 0.19%) and control (1.43% ± 0.18%) groups (p<0.023).ConclusionsIntermittent systolic overload promotes greater upregulation of VEGF expression in the subpulmonary ventricle, an adaptation that provides a mechanism for increased myocardial perfusion during the rapid myocardial hypertrophy of young goats.

scholarly journals Comparison of Menstrual Effluent Vascular Endothelial Growth Factor Immunocytochemistry Expression Between Endometriosis and Non-Endometriosis Patients

2020 ◽  
Vol 9 (3) ◽  
pp. 182-189
Author(s):  
Ruswana Anwar ◽  
Sunardi Sunardi ◽  
Siti Salima ◽  
Setyorini Irianti ◽  
Benny Hasan Purwara ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Study Groups ◽  
Pain Scale ◽  
Control Group ◽  
Vascular Endothelial ◽  
Vegf Expression ◽  
Cyst Size ◽  
Significant Difference ◽  
Endothelial Growth Factor

Objectives: This study aimed to measure the vascular endothelial growth factor (VEGF) expression in menstrual effluent from patients with endometriosis compared to non-endometriosis through immunocytochemical methods. We also measured the degree of pain level, endometrioma cyst size, and infertility status whether it is affected by VEGF expression. Materials and Methods: The present case-control study was conducted in Hasan Sadikin General Hospital, Bandung. Thirty productive-age women diagnosed with endometrioma and 30 productive-age women without endometriosis as the control group were included in this study. Menstrual effluent was taken from the posterior fornix on the second day of menstruation and stained using immunocytochemistry staining for VEGF. Results: The results demonstrated a significant difference between the two study groups in terms of VEGF intensity and histoscore although no difference was found in VEGF distributions between the study groups. The subjects in the endometriosis group had significantly higher VEGF intensity and significantly higher VEGF histoscore compared to the control group. Women with VEGF histoscore more than 6 has 9.33 times risk of developing endometriosis compared to those with lower histoscore. There were no significant correlations between VEGF and pain scale, infertility, and the cyst size. Finally, the cyst size was proportionally related to pain. Conclusions: VEGF distribution and expression in endometriosis women were significantly higher than VEGF levels in non-endometriosis women. Women with menstrual effluent containing higher VEGF levels had more chances of developing endometriosis compared to those with lower VEGF levels. Eventually, larger endometrioma size was proportionally related to higher pain levels in subjects with endometrioma.

scholarly journals Enhanced Vascular Endothelial Growth Factor Gene Expression in Ischaemic Skin of Critical Limb Ischaemia Patients

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Silvia Bleda ◽  
Joaquín de Haro ◽  
Francisco Acin ◽  
César Varela ◽  
Leticia Esparza
Keyword(s):  
Gene Expression ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Critical Limb Ischaemia ◽  
Control Group ◽  
Venous Disease ◽  
Vascular Endothelial ◽  
Vegf Gene ◽  
Vegf Expression ◽  
Endothelial Growth Factor

Objectives. To perform a quantitative analysis of the vascular endothelial growth factor (VEGF) gene transcription in the skin of ischemic legs and provide information for VEGF in the pathogenesis in critical limb ischemia (CLI).Methods. Skin biopsies were obtained from 40 patients with CLI. Control samples came from 44 patients with chronic venous disease. VEGF gene expression was analysed using quantitative polymerase chain reaction.Results. Patients with CLI had higher skin VEGF expression than control group (RQ: 1.3 ± 0.1 versus 1,P=0.04).Conclusions. We found an association between ischemic skin and an elevated VEGF expression in legs from patients with CLI. These data support that the mechanism for VEGF upregulation in hypoxia conditions is intact and acts appropriately in the ischaemic limbs from patients with CLI.

Effect of training on the response of plasma vascular endothelial growth factor to exercise in patients with peripheral arterial disease

2006 ◽  
Vol 111 (6) ◽  
pp. 401-409 ◽  
Author(s):  
Rachel E. Wood ◽  
Brad E. Sanderson ◽  
Christopher D. Askew ◽  
Philip J. Walker ◽  
Simon Green ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Exercise Training ◽  
Acute Exercise ◽  
Oxygen Demand ◽  
Control Group ◽  
Vascular Endothelial ◽  
Walking Test ◽  
Before And After ◽  
Endothelial Growth Factor

Expansion of the capillary network, or angiogenesis, occurs following endurance training. This process, which is reliant on the presence of VEGF (vascular endothelial growth factor), is an adaptation to a chronic mismatch between oxygen demand and supply. Patients with IC (intermittent claudication) experience pain during exercise associated with an inadequate oxygen delivery to the muscles. Therefore the aims of the present study were to examine the plasma VEGF response to acute exercise, and to establish whether exercise training alters this response in patients with IC. In Part A, blood was collected from patients with IC (n=18) before and after (+20 and +60 min post-exercise) a maximal walking test to determine the plasma VEGF response to acute exercise. VEGF was present in the plasma of patients (45.11±29.96 pg/ml) and was unchanged in response to acute exercise. Part B was a training study to determine whether exercise training altered the VEGF response to acute exercise. Patients were randomly assigned to a treatment group (TMT; n=7) that completed 6 weeks of high-intensity treadmill training, or to a control group (CON; n=6). All patients completed a maximal walking test before and after the intervention, with blood samples drawn as for Part A. Training had no effect on plasma VEGF at rest or in response to acute exercise, despite a significant increase in maximal walking time in the TMT group (915±533 to 1206±500 s; P=0.009) following the intervention. The absence of a change in plasma VEGF may reflect altered VEGF binding at the endothelium, although this cannot be confirmed by the present data.

scholarly journals Effects of hyperthyroidism on expression of vascular endothelial growth factor (VEGF) and apoptosis in fetal adrenal glands

2015 ◽  
Vol 59 (4) ◽  
Author(s):  
T. Karaca ◽  
Y. Hulya Uz ◽  
R. Karabacak ◽  
I. Karaboga ◽  
S. Demirtas ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Adrenocorticotropic Hormone ◽  
Adrenal Glands ◽  
Control Group ◽  
Vascular Density ◽  
Vascular Endothelial ◽  
Vegf Expression ◽  
Fetal Rat ◽  
Endothelial Growth Factor

<p>This study investigated the expression of vascular endothelial growth factor (VEGF), vascular density, and apoptosis in fetal rat adrenal glands with hyperthyroidism in late gestation. Twelve mature female Wistar albino rats with the same biological and physiological features were used for this study. Rats were divided into two groups: control and hyperthyroidism. Hyperthyroidism was induced by daily subcutaneous injections of L-thyroxine (250 μg/kg) before pregnancy for 21 days and during pregnancy. Rats in the control and hyperthyroidism groups were caged according to the number of male rats. Zero day of pregnancy (Day 0) was indicated when the animals were observed to have microscopic sperm in vaginal smears. Pregnant rats were sacrificed on the 20th day of pregnancy; blood from each animal was collected to determine the concentrations of maternal adrenocorticotropic hormone and thyroxine. Rat fetuses were then quickly removed from the uterus, and the adrenal glands of the fetuses were dissected. VEGF expression, vascular density, and apoptosis were analyzed in fetal rat adrenal glands. Maternal serum levels of the adrenocorticotropic hormone and free thyroxine were significantly higher in the hyperthyroidism group than in the control group. Immunohistochemistry revealed that the number of VEGF positive cells and vessel density significantly increased in the hyperthyroidism rat fetal adrenal group compared with the control group. Hyperthyroidism did not change the fetal and placental weights and the number of fetuses. This study demonstrates that hyperthyroidism may have an effect on the development of rat adrenal glands mediated by VEGF expression, angiogenesis, and apoptosis. </p>

scholarly journals Evaluation of vascular endothelial growth factor and nitric oxide associated with resveratrol supplementation

2021 ◽  
Vol 31 (Supplement_2) ◽  
Author(s):  
Armando Caseiro ◽  
Carla Ferreira ◽  
Ana Margarida Silva ◽  
João Paulo Figueiredo ◽  
Telmo Pereira
Keyword(s):  
Nitric Oxide ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Intervention Group ◽  
Study Groups ◽  
Control Group ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor

Abstract Background Resveratrol (3,4',5-trihydroxystilbene) (RSV) is one of the main non-flavonoid natural polyphenol compounds. Evidence suggests that RSV has a key role in preventing a variety of pathological processes because of its benefits, including anti-aging, anti-inflammatory, and cardiovascular disease prevention. Vascular endothelial growth factor (VEGF) is responsible for vasculogenesis and angiogenesis, and its expression is influenced by RSV. Vascular nitric oxide (NO) acts to relax smooth muscle cells by preventing thrombogenic processes. It has been shown in vitro and in vivo that RSV is involved in NO metabolism. The aim of this work was evaluate the effects of regular low-dose RSV consumption by determining serum VEGF and NO levels compared to a control group. Methods The study involved 27 clinically healthy individuals, divided into a control group (placebo) and an intervention group, supplemented with 100mg RSV/day. The VEGF levels were determined by slot blot technique and NO levels were determined by spectrophotometry before (T0) and after 30 days (T1) of supplementation. Results The VEGF and NO levels slightly decrease from T0 to T1 moment in both study groups, showing a higher decrease in both parameters in the control group compared to the intervention group, but the variation was not statistically significant. Conclusions Daily supplementation with RSV is associated with benefits at the VEGF level as well as at the vascular level. However, further studies with a larger number of participants are needed to confirm the effects of RSV on VEGF and NO levels.

scholarly journals Increased Vascular Endothelial Growth Factor (VEGF) Expression in Wistar Rat Oral Mucosa Traumatic Ulcer due to Golden Sea Cucumber (Stichopus hermanni) Extract Gel

2019 ◽  
Vol 53 (6) ◽  
Author(s):  
Pratiwi Soesilawati ◽  
Eghia Laditra Ambarani ◽  
Yuliati ◽  
Aqsa Sjuhada Oki
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Wound Healing ◽  
Growth Factor ◽  
Sea Cucumber ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Vascular Endothelial ◽  
Vegf Expression ◽  
Endothelial Growth Factor

Background. Traumatic ulcer is a lesion formed by a local tissue damage due to trauma on epithelial layer. Vascular Endothelial Growth Factor (VEGF) plays an important role in wound healing, especially in angiogenesis. Golden sea cucumber (Stichopus hermanni) is believed to accelerate the wound healing process. Objective. To prove that golden sea cucumber extract can increase VEGF expression in oral mucous membrane traumatic ulcer in rat.Method. S. hermanni extract was prepared by freeze-dry method, then an extract was made using PEG 400 or PEG 4000 at 40% and 80% concentrations, respectively, and applied to the animal’s oral wounds. The animals were divided into three groups: control; 40% S. hermanni extract gel; and 80% S. hermanni extract gel. The ulcers that formed on day 3 were rubbed gently with S. hermanni extract gel. After being sacrificed on day 4, sample tissues from the lower lips were prepared for histopathology to count the number of VEGF expression. The results were analyzed using the One-Way ANOVA statistical test. Results. A significant increase in the VEGF expression of 80% concentration S. hermanni extract gel was found compared with those in the control group (p=0.00). Conclusion. Golden sea cucumber extract (Stichopus hermanni) gel increased the VEGF expression in oral mucous traumatic ulcer.

scholarly journals Vascular endothelial growth factor (VEGF) expression in dogs suffering from squamous cell carcinoma

2017 ◽  
Vol 73 (5) ◽  
pp. 289-294
Author(s):  
Aleksandra Sobczyńska-Rak ◽  
Izabela Polkowska ◽  
Magdalena Gołyńska ◽  
Tomasz Szponder ◽  
Beata Żylińska ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Squamous Cell Carcinoma ◽  
Growth Factor ◽  
Blood Serum ◽  
Control Group ◽  
Study Group ◽  
Vascular Endothelial ◽  
Vegf Expression ◽  
Blood Samples ◽  
Endothelial Growth Factor

The aim of the present study was to monitor VEGF expression in the blood serum of dogs suffering from squamous cell carcinoma in correlation with the speed of metastasis. The research material consisted of tumour tissue and blood samples collected from 26 dogs of various breeds, aged between 5 and 15 years, and 10 control group animals. Blood samples were centrifuged, and the serum obtained was examined by immunoenzymatic assays to determine vascular endothelial growth factor (VEGF) levels. The study group animals were observed for a period of 6 months, during which their blood was drawn twice for immunoenzymatic tests. In all study group animals, elevated blood serum VEGF levels were observed on the day of surgery compared with the control group. In 14 cases, disease relapse was observed with metastases to the bones or lungs. In all relapsing animals, significantly higher blood serum VEGF levels were observed. VEGF levels are closely related to the neoplastic process, but the exact mechanism of the factor's involvement in neoplastic progression remains unclear, as does the complexity of the angiogenic processes. Studies of the mechanisms affecting tumour proliferation and the monitoring of VEGF levels can facilitate better diagnostics and prognostics, as well as improve the effectiveness of cancer treatment and the planning of new therapeutic strategies for both human and animal patients.

scholarly journals Follicular development and the expression of BAX and vascular endothelial growth factor in transplanted ovaries in uni- and bilateral ovariectomized mice: An experimental study

2021 ◽  
Author(s):  
Maryam Dehghan ◽  
Shirin Shahbazi ◽  
Mojdeh Salehnia
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Follicular Development ◽  
Control Group ◽  
Vascular Endothelial ◽  
Vegf Expression ◽  
Intact Control ◽  
Antral Follicles ◽  
Ovariectomized Mice ◽  
Endothelial Growth Factor

Background: Several conflicting results have been reported on the survival and function of transplanted ovaries. Objective: Evaluation of the follicular development and the expression of vascular endothelial growth factor (VEGF) and Bcl-2-associated X protein (BAX) in ovaries transplanted into uni- and bilaterally ovariectomized mice. Materials and Methods: In this experimental study, 40 female NMRI mice (21-days-old, 12-15 gr) were ovariectomized uni- and bilaterally (n = 20/ group), while the 8-wk-old mice were considered as intact control group (n = 6). 5 weeks after transplantation at the proestrus stage, the morphology of recovered transplanted ovaries and the proportion of follicles were studied at different developmental stages. The apoptosis cell death by pro-apoptotic protein BAX and the expression of VEGF were evaluated using immunohistochemistry. Results: In the bilaterally ovariectomized mice, among the 455 counted normal follicles, a lower rate of primordial and primary follicles and a higher rate of preantral and antral follicles were observed (p = 0.002). However, the percentages of preantral and antral follicles, and the corpus luteum were significantly lower in the intact control group (among the 508 counted normal follicles in this group) compared to other transplanted groups (p = 0.002). The number of BAX-positive cells in all groups was not significantly different. The VEGF expression was prominent in vessels of the corpus luteum, and also in the theca layer of large follicles of studied groups. Conclusion: Early discharge of ovarian reserve was prominent in the bilaterally ovariectomized group but the incidence of apoptotic cells and VEGF expression as angiogenic factor did not differ in both ovariectomized mice. Thus, unilaterally ovariectomy has less side effects on the ovarian reserve compared to bilateral ovariectomy. Key words: Autotransplantation, BAX protein, Vascular endothelial growth factor, Ovariectomy, Mice.

scholarly journals Effect of anaerobic gymnastics exercise on vascular endothelial growth factor in obese boys

2020 ◽  
Vol 60 (6) ◽  
pp. 293-302
Author(s):  
Vahid Saleh ◽  
Roghayye Afroundeh ◽  
Marefat Siahkohiyan ◽  
Asadollah Asadi
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Body Fat ◽  
Normal Weight ◽  
Lean Body Weight ◽  
Control Group ◽  
Vascular Endothelial ◽  
Before And After ◽  
Obese Control ◽  
Endothelial Growth Factor

Background Vascular endothelial growth factor (VEGF) is the most important growth factor involved in angiogenesis and appears to be mediated through exercise training, leading to increased blood lactate. Objective To evaluate and compare the effects of anaerobic gymnastics exercise (AGE) on systemic VEGF in obese and normoweight boys.  Methods Sixty boys aged 8 to 12 years who enrolled in elementary level of gymnastics participated in this study and were randomly divided into four groups of 15 subjects each: obese AGE, obese control, normoweight AGE, and normoweight control. The control group didn’t have any exercise during the study. The experimental groups performed 45 minutes of AGE 3 times per week for 8 weeks, which included a 10-minute warm-up, 30-minute main exercises, and 5-minute cool down. Body composition characteristics and VEGF levels in saliva were measured before and after 8 weeks of training. Results Significant changes following AGE were found in the obese group in terms of weight (-8.09%; P=0.001), body fat% (BF%) (-12.81; P=0.001), body fat weight (BFW) (-19.38; P=0.001), and lean body weight (LBW) (-3.20; P=0.001). Saliva levels of VEGF increased post-AGE in the obese (+21.64%; P=0.79) and normoweight groups (+28.22; P=0.06), but the differences were not significant. Significant differences in weight, BF%, and BFW were found in between obese AGE group with obese control, normoweight control and normoweight AGE (P˂0.05). Conclusion Circulating VEGF concentrations slightly increase after 8 weeks of AGE in obese and normal-weight groups. Moreover, we demonstrate that weight significantly decreased in obese children after they engaged in AGE training.

Sign in / Sign up

Export Citation Format

Share Document