scholarly journals Vascular endothelial growth factor (VEGF) expression in dogs suffering from squamous cell carcinoma

2017 ◽  
Vol 73 (5) ◽  
pp. 289-294
Author(s):  
Aleksandra Sobczyńska-Rak ◽  
Izabela Polkowska ◽  
Magdalena Gołyńska ◽  
Tomasz Szponder ◽  
Beata Żylińska ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Squamous Cell Carcinoma ◽  
Growth Factor ◽  
Blood Serum ◽  
Control Group ◽  
Study Group ◽  
Vascular Endothelial ◽  
Vegf Expression ◽  
Blood Samples ◽  
Endothelial Growth Factor

The aim of the present study was to monitor VEGF expression in the blood serum of dogs suffering from squamous cell carcinoma in correlation with the speed of metastasis. The research material consisted of tumour tissue and blood samples collected from 26 dogs of various breeds, aged between 5 and 15 years, and 10 control group animals. Blood samples were centrifuged, and the serum obtained was examined by immunoenzymatic assays to determine vascular endothelial growth factor (VEGF) levels. The study group animals were observed for a period of 6 months, during which their blood was drawn twice for immunoenzymatic tests. In all study group animals, elevated blood serum VEGF levels were observed on the day of surgery compared with the control group. In 14 cases, disease relapse was observed with metastases to the bones or lungs. In all relapsing animals, significantly higher blood serum VEGF levels were observed. VEGF levels are closely related to the neoplastic process, but the exact mechanism of the factor's involvement in neoplastic progression remains unclear, as does the complexity of the angiogenic processes. Studies of the mechanisms affecting tumour proliferation and the monitoring of VEGF levels can facilitate better diagnostics and prognostics, as well as improve the effectiveness of cancer treatment and the planning of new therapeutic strategies for both human and animal patients.

scholarly journals Vascular endothelial growth factor in prognosis of splenic malignant tumours in dogs

2014 ◽  
Vol 58 (2) ◽  
pp. 255-260
Author(s):  
Aleksandra Sobczyńska-Rak ◽  
Izabela Polkowska ◽  
Adam Brodzki
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Comparative Analysis ◽  
Growth Factor ◽  
Who Classification ◽  
Control Group ◽  
Vascular Endothelial ◽  
Malignant Tumours ◽  
Blood Samples ◽  
Endothelial Growth Factor ◽  
Immunoenzymatic Assays

Abstract The aim of the study was to determine the levels of the vascular endothelial growth factor (VEGF) in the serum of dogs suffering from splenic malignant tumours, prior to splenectomy, as well as three and six months after the surgery. Tumours and blood samples were collected from 10 dogs of various breeds, aged between 7 and 13 years, and from 10 control animals. Tumour sections were fixed in 10% buffered formalin for 24 h. The type of tumour was determined according to the WHO classification. Blood samples were centrifuged and the obtained sera were subjected to immunoenzymatic assays to determine the VEGF levels. The median of VEGF levels in the serum of dogs suffering from splenic malignant tumours was 37.85 pg/mL (15.40-107.18 pg/mL). The highest values were observed in dogs with confirmed metastases (107.18 pg/mL and 65.43 pg/mL). The VEGF values in control group were between 0.1 pg/mL and 13.04 pg/mL. A comparative analysis of the VEGF levels against the animals' survival time indicated that VEGF overexpression may serve as a prognostic factor in cases of malignant tumours of the spleen.

scholarly journals Enhanced Vascular Endothelial Growth Factor Gene Expression in Ischaemic Skin of Critical Limb Ischaemia Patients

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Silvia Bleda ◽  
Joaquín de Haro ◽  
Francisco Acin ◽  
César Varela ◽  
Leticia Esparza
Keyword(s):  
Gene Expression ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Critical Limb Ischaemia ◽  
Control Group ◽  
Venous Disease ◽  
Vascular Endothelial ◽  
Vegf Gene ◽  
Vegf Expression ◽  
Endothelial Growth Factor

Objectives. To perform a quantitative analysis of the vascular endothelial growth factor (VEGF) gene transcription in the skin of ischemic legs and provide information for VEGF in the pathogenesis in critical limb ischemia (CLI).Methods. Skin biopsies were obtained from 40 patients with CLI. Control samples came from 44 patients with chronic venous disease. VEGF gene expression was analysed using quantitative polymerase chain reaction.Results. Patients with CLI had higher skin VEGF expression than control group (RQ: 1.3 ± 0.1 versus 1,P=0.04).Conclusions. We found an association between ischemic skin and an elevated VEGF expression in legs from patients with CLI. These data support that the mechanism for VEGF upregulation in hypoxia conditions is intact and acts appropriately in the ischaemic limbs from patients with CLI.

scholarly journals Comparison of Menstrual Effluent Vascular Endothelial Growth Factor Immunocytochemistry Expression Between Endometriosis and Non-Endometriosis Patients

2020 ◽  
Vol 9 (3) ◽  
pp. 182-189
Author(s):  
Ruswana Anwar ◽  
Sunardi Sunardi ◽  
Siti Salima ◽  
Setyorini Irianti ◽  
Benny Hasan Purwara ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Study Groups ◽  
Pain Scale ◽  
Control Group ◽  
Vascular Endothelial ◽  
Vegf Expression ◽  
Cyst Size ◽  
Significant Difference ◽  
Endothelial Growth Factor

Objectives: This study aimed to measure the vascular endothelial growth factor (VEGF) expression in menstrual effluent from patients with endometriosis compared to non-endometriosis through immunocytochemical methods. We also measured the degree of pain level, endometrioma cyst size, and infertility status whether it is affected by VEGF expression. Materials and Methods: The present case-control study was conducted in Hasan Sadikin General Hospital, Bandung. Thirty productive-age women diagnosed with endometrioma and 30 productive-age women without endometriosis as the control group were included in this study. Menstrual effluent was taken from the posterior fornix on the second day of menstruation and stained using immunocytochemistry staining for VEGF. Results: The results demonstrated a significant difference between the two study groups in terms of VEGF intensity and histoscore although no difference was found in VEGF distributions between the study groups. The subjects in the endometriosis group had significantly higher VEGF intensity and significantly higher VEGF histoscore compared to the control group. Women with VEGF histoscore more than 6 has 9.33 times risk of developing endometriosis compared to those with lower histoscore. There were no significant correlations between VEGF and pain scale, infertility, and the cyst size. Finally, the cyst size was proportionally related to pain. Conclusions: VEGF distribution and expression in endometriosis women were significantly higher than VEGF levels in non-endometriosis women. Women with menstrual effluent containing higher VEGF levels had more chances of developing endometriosis compared to those with lower VEGF levels. Eventually, larger endometrioma size was proportionally related to higher pain levels in subjects with endometrioma.

scholarly journals Effects of hyperthyroidism on expression of vascular endothelial growth factor (VEGF) and apoptosis in fetal adrenal glands

2015 ◽  
Vol 59 (4) ◽  
Author(s):  
T. Karaca ◽  
Y. Hulya Uz ◽  
R. Karabacak ◽  
I. Karaboga ◽  
S. Demirtas ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Adrenocorticotropic Hormone ◽  
Adrenal Glands ◽  
Control Group ◽  
Vascular Density ◽  
Vascular Endothelial ◽  
Vegf Expression ◽  
Fetal Rat ◽  
Endothelial Growth Factor

<p>This study investigated the expression of vascular endothelial growth factor (VEGF), vascular density, and apoptosis in fetal rat adrenal glands with hyperthyroidism in late gestation. Twelve mature female Wistar albino rats with the same biological and physiological features were used for this study. Rats were divided into two groups: control and hyperthyroidism. Hyperthyroidism was induced by daily subcutaneous injections of L-thyroxine (250 μg/kg) before pregnancy for 21 days and during pregnancy. Rats in the control and hyperthyroidism groups were caged according to the number of male rats. Zero day of pregnancy (Day 0) was indicated when the animals were observed to have microscopic sperm in vaginal smears. Pregnant rats were sacrificed on the 20th day of pregnancy; blood from each animal was collected to determine the concentrations of maternal adrenocorticotropic hormone and thyroxine. Rat fetuses were then quickly removed from the uterus, and the adrenal glands of the fetuses were dissected. VEGF expression, vascular density, and apoptosis were analyzed in fetal rat adrenal glands. Maternal serum levels of the adrenocorticotropic hormone and free thyroxine were significantly higher in the hyperthyroidism group than in the control group. Immunohistochemistry revealed that the number of VEGF positive cells and vessel density significantly increased in the hyperthyroidism rat fetal adrenal group compared with the control group. Hyperthyroidism did not change the fetal and placental weights and the number of fetuses. This study demonstrates that hyperthyroidism may have an effect on the development of rat adrenal glands mediated by VEGF expression, angiogenesis, and apoptosis. </p>

scholarly journals Elevated Vascular Endothelial Growth Factor (VEGF) levels in the blood serum of dogs with malignant neoplasms of the oral cavity

2014 ◽  
Vol 62 (3) ◽  
pp. 362-371 ◽  
Author(s):  
Aleksandra Sobczyńska-Rak ◽  
Izabela Polkowska ◽  
Piotr Silmanowicz
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Oral Cavity ◽  
Blood Serum ◽  
Control Group ◽  
Malignant Neoplasms ◽  
Vascular Endothelial ◽  
Neoplastic Tissue ◽  
Tissue Samples ◽  
Endothelial Growth Factor

Angiogenesis plays an essential role in the development of a neoplastic tumour by conditioning both its growth and the formation of metastases. The induction of blood vessel growth occurs under the influence of proangiogenic factors, among which Vascular Endothelial Growth Factor (VEGF) seems to be the most important. The aim of this research was to study the level of VEGF measured by ELISA in the serum of dogs with neoplasms of the oral cavity. The study material comprised samples of neoplastic tissue from 17 operated dogs and the serum of the examined animals as well as of dogs from the control group. The tissue samples were taken from dogs of different breeds, aged 6–14 years. The tumour type was determined in accordance with the applicable WHO classification. Blood samples taken from sick dogs and from animals of the control group were centrifuged, and immunoenzymatic labelling of VEGF was performed in the obtained serum using ELISA and R&D system reagents (Quantikine Canine VEGF). All stages of VEGF labelling were performed according to the recommendation of the test manufacturer. The median of VEGF in the serum of the dogs with neoplasms of the oral cavity was 40.64 pg/mL. The lowest value of 14.26 pg/mL was observed in the case of fibrosarcoma, and the highest value of 99.19 pg/mL in the case of squamous cell carcinoma. The VEGF median in the control group amounted to 11.14 pg/mL whereas the VEGF value in the groups of animals diagnosed with benign tumours ranged between 2.30 and 19.74 pg/mL. Elevated VEGF in the blood serum, in comparison with the benign tumour group and the control group, was observed in all examined neoplasms of the oral cavity. It was suggested that overexpression of VEGF can have a prognostic value and is useful in the early detection of neoplasms.

scholarly journals Increased Vascular Endothelial Growth Factor (VEGF) Expression in Wistar Rat Oral Mucosa Traumatic Ulcer due to Golden Sea Cucumber (Stichopus hermanni) Extract Gel

2019 ◽  
Vol 53 (6) ◽  
Author(s):  
Pratiwi Soesilawati ◽  
Eghia Laditra Ambarani ◽  
Yuliati ◽  
Aqsa Sjuhada Oki
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Wound Healing ◽  
Growth Factor ◽  
Sea Cucumber ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Vascular Endothelial ◽  
Vegf Expression ◽  
Endothelial Growth Factor

Background. Traumatic ulcer is a lesion formed by a local tissue damage due to trauma on epithelial layer. Vascular Endothelial Growth Factor (VEGF) plays an important role in wound healing, especially in angiogenesis. Golden sea cucumber (Stichopus hermanni) is believed to accelerate the wound healing process. Objective. To prove that golden sea cucumber extract can increase VEGF expression in oral mucous membrane traumatic ulcer in rat.Method. S. hermanni extract was prepared by freeze-dry method, then an extract was made using PEG 400 or PEG 4000 at 40% and 80% concentrations, respectively, and applied to the animal’s oral wounds. The animals were divided into three groups: control; 40% S. hermanni extract gel; and 80% S. hermanni extract gel. The ulcers that formed on day 3 were rubbed gently with S. hermanni extract gel. After being sacrificed on day 4, sample tissues from the lower lips were prepared for histopathology to count the number of VEGF expression. The results were analyzed using the One-Way ANOVA statistical test. Results. A significant increase in the VEGF expression of 80% concentration S. hermanni extract gel was found compared with those in the control group (p=0.00). Conclusion. Golden sea cucumber extract (Stichopus hermanni) gel increased the VEGF expression in oral mucous traumatic ulcer.

scholarly journals Follicular development and the expression of BAX and vascular endothelial growth factor in transplanted ovaries in uni- and bilateral ovariectomized mice: An experimental study

2021 ◽  
Author(s):  
Maryam Dehghan ◽  
Shirin Shahbazi ◽  
Mojdeh Salehnia
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Follicular Development ◽  
Control Group ◽  
Vascular Endothelial ◽  
Vegf Expression ◽  
Intact Control ◽  
Antral Follicles ◽  
Ovariectomized Mice ◽  
Endothelial Growth Factor

Background: Several conflicting results have been reported on the survival and function of transplanted ovaries. Objective: Evaluation of the follicular development and the expression of vascular endothelial growth factor (VEGF) and Bcl-2-associated X protein (BAX) in ovaries transplanted into uni- and bilaterally ovariectomized mice. Materials and Methods: In this experimental study, 40 female NMRI mice (21-days-old, 12-15 gr) were ovariectomized uni- and bilaterally (n = 20/ group), while the 8-wk-old mice were considered as intact control group (n = 6). 5 weeks after transplantation at the proestrus stage, the morphology of recovered transplanted ovaries and the proportion of follicles were studied at different developmental stages. The apoptosis cell death by pro-apoptotic protein BAX and the expression of VEGF were evaluated using immunohistochemistry. Results: In the bilaterally ovariectomized mice, among the 455 counted normal follicles, a lower rate of primordial and primary follicles and a higher rate of preantral and antral follicles were observed (p = 0.002). However, the percentages of preantral and antral follicles, and the corpus luteum were significantly lower in the intact control group (among the 508 counted normal follicles in this group) compared to other transplanted groups (p = 0.002). The number of BAX-positive cells in all groups was not significantly different. The VEGF expression was prominent in vessels of the corpus luteum, and also in the theca layer of large follicles of studied groups. Conclusion: Early discharge of ovarian reserve was prominent in the bilaterally ovariectomized group but the incidence of apoptotic cells and VEGF expression as angiogenic factor did not differ in both ovariectomized mice. Thus, unilaterally ovariectomy has less side effects on the ovarian reserve compared to bilateral ovariectomy. Key words: Autotransplantation, BAX protein, Vascular endothelial growth factor, Ovariectomy, Mice.

scholarly journals Expression levels of circulatory mir-185-5p, vascular endothelial growth factor, and platelet-derived growth factor target genes in endometriosis

2020 ◽  
Author(s):  
Mohammad Hossein Razi ◽  
Maryam Eftekhar ◽  
Nasrin Ghasemi ◽  
Mohammad Hasan Sheikhha ◽  
Ali Dehghani Firoozabadi
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Target Genes ◽  
Platelet Derived Growth Factor ◽  
Control Group ◽  
Case Group ◽  
Vascular Endothelial ◽  
Blood Samples ◽  
Qrt Pcr ◽  
Endothelial Growth Factor

Background: Using blood-based biomarkers such as microRNAs (miRNAs) may allow particularly effective and minimally invasive diagnosis and treatment of endometriosis. Objective: We evaluated the differential expression of circulating miRNA-185-5p (miR- 185-5p), vascular endothelial growth factor (VEGF), and platelet-derived growth factor (PDGF) target genes between endometriosis and healthy women. Materials and Methods: 25 women with a history of endometriosis (grad III-IV) diagnosed by laparoscopy as the case group and 25 women without endometriosis underwent laparoscopy for ovarian cysts or pelvic pain as the control group were enrolled in this case-control study. Blood samples were obtained, and total RNA was used for high-throughput small RNA sequencing, and this was confirmed by means of quantitative real-time polymerase chain reaction (qRT-PCR). Results: miRNA expression profiling using non-coding RNA sequencing revealed that one miRNA including miR-185-5p was significantly down-regulated in the case group compared with the controls. The qRT-PCR results showed significant downregulation of the expression level of miR-185-5p (p < 0.01) in the plasma of the case group. Receiver operating characteristic (ROC) curve analysis showed the area of miR-185-5p under the ROC curve for endometriosis diagnosis was 0.919 (p < 0.001). The RT-PCR results demonstrated that there was no significant difference in the expression of VEGF and PDGF mRNA of blood samples in the cases compared to the control group (PDGF, p = 0.09 and VEGF, p = 0.36). Conclusion: The low expression of miR-185-5p in the plasma of women with endometriosis could be employed as an important non-invasive biomarker for early detection and screening of endometriosis by blood samples. Key words: Biomarker, miRNA, Diagnosis, Endometriosis, Angiogenesis.

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