scholarly journals Toggling Geobacter sulfurreducens metabolic state reveals hidden behaviour and expanded applicability to sustainable energy applications

2019 ◽  
Author(s):  
Mir Pouyan Zarabadi ◽  
Steve J. Charette ◽  
Jesse Greener
Keyword(s):  
Real Time ◽  
Metabolic Activity ◽  
Energy Production ◽  
Geobacter Sulfurreducens ◽  
Metabolic State ◽  
Flow Reactors ◽  
Energy Applications ◽  
Sustainable Technologies ◽  
Low Concentrations ◽  
Metabolic States

AbstractElectroactive biofilms are under intense scrutiny due to their potential to enable new sustainable technologies for energy production and bioremediation. However, severely reduced metabolic activity at low concentrations is a barrier to their implementation. A microfluidic approach was used for real-time respiration experiments on a Geobacter sulfurreducens biofilm to overcome these constraints. Precise changes to solution conditions enabled rapid and reversible switching between biofilm metabolic states, leading to the following discoveries. (i) Flow reactors can maintain biofilm activity at concentrations as low as 15 µM; (ii) a “pseudo-active” metabolic state separates active and inactive states; and (iii) acetate conversion can be as high as 90 percent for active biofilms at the pseudo-activity threshold.

scholarly journals Toggling Geobacter sulfurreducens metabolic state reveals hidden behaviour and expanded applicability to sustainable energy applications

2019 ◽  
Vol 3 (9) ◽  
pp. 2211-2217 ◽  
Author(s):  
Mir Pouyan Zarabadi ◽  
Steve J. Charette ◽  
Jesse Greener
Keyword(s):  
Metabolic Activity ◽  
Sustainable Energy ◽  
Geobacter Sulfurreducens ◽  
Metabolic State ◽  
Energy Applications ◽  
Low Concentrations

Geobacter biofilms can be switched into different metabolic activity states and operate efficiently at low concentrations microfluidic three-electrode reactor.

Robotics Application in Remote Data Acquisition and Control for Solar Ponds

2012 ◽  
Vol 253-255 ◽  
pp. 705-715 ◽  
Author(s):  
Mohamed Elbanhawi ◽  
Milan Simic
Keyword(s):  
Data Acquisition ◽  
Real Time ◽  
Energy Production ◽  
Green Energy ◽  
Industrial Robots ◽  
Sampling Station ◽  
Monitoring And Control ◽  
Solar Ponds ◽  
And Control ◽  
Remote Data

This paper presents one application of industrial robots in the automation of renewable energy production. The robot supports remote performance monitoring and maintenance of salinity gradient solar ponds. The details of the design, setup and the use of the robot sampling station and the remote Data Acquisition (DAQ) system are given here. The use of a robot arm, to position equipment and sensors, provides accurate and reliable real time data needed for autonomous monitoring and control of this type of green energy production. Robot upgrade of solar ponds can be easily integrated with existing systems. Data logged by the proposed system can be remotely accessed, plotted and analysed. Thus the simultaneous and remote monitoring of a large scale network of ponds can be easily implemented. This provides a fully automated solution to the monitoring and control of green energy production operations, which can be used to provide heat and electricity to buildings. Remote real time monitoring will facilitate the setup and operations of several solar ponds around cities.

scholarly journals A vércukorértékek nem feltétlenül jellemzik megfelelően a sejtanyagcsere-állapotot

2017 ◽  
Vol 158 (11) ◽  
pp. 409-417
Author(s):  
Kornél Simon ◽  
István Wittmann
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Energy Production ◽  
Cellular Metabolism ◽  
Transport Parameter ◽  
Metabolic State ◽  
Cellular Energy ◽  
Acute And Chronic Stress

Abstract: In clinical recommendations the normalized blood glucose level is declared as the main target in therapy of diabetes mellitus, i.e. the achievement of euglycemia is the main therapeutic goal. This approach suggests, that the normal blood glucose value is the marker of the normal carbohydrate metabolism (eumetabolism), and vice versa: hyperglycemia is associated with abnormal metabolism (dysmetabolism). However the question arises, whether identical blood glucose values do reflect the same intracellular biochemical mechanisms? On the basis of data published in the literature authors try to answer these questions by studying the relations between the short/longterm blood glucose level and the cellular metabolism in different clinical settings characterized by divergent pathophysiological parameters. The correlations between blood glucose level and cellular metabolism in development of micro-, and macroangiopathy, in the breakthrough phenomenon, as well as during administration of metabolic promoters, the discrepancies of relation between blood glucose values and cellular metabolism in type 1, and type 2 diabetes mellitus, furthermore association between blood glucose value and myocardial metabolism in acute and chronic stress were analyzed. Authors conclude, that the actual blood glucose values reveal the actual cellular metabolism in a very variable manner: neither euglycemia does mandatorily indicate eumetabolism (balance of cellular energy production), nor hyperglycemia is necessarily a marker of abnormal metabolic state (dept of cellular energy production). Moreover, at the same actual blood glucose level both the metabolic efficacy of the same organ may sharply vary, and the intracellular biochemical machinery could also be very different. In case of the very same longterm blood glucose level the metabolic state of the different organs could be very variable: some organs show an energetically balanced metabolism, while others produce a significant deficit. These inconsistencies between blood glucose level and cellular metabolism can be explained by the fact, that blood glucose value is a transport parameter, reflecting the actual steady state of glucose transport from the carbohydrate pools into the blood, and that from the blood into the tissues. Without knowing the speed of these transports of opposite direction, the blood glucose value per se can not reveal the quantitative and qualitative characteristics of cellular metabolism. Orv. Hetil., 2017, 158(11), 409–417.

Synthesis of Tailored Segmented Polyurethanes Utilizing Continuous-Flow Reactors and Real-Time Process Monitoring

2021 ◽  
Author(s):  
Xabier Lopez de Pariza ◽  
Tim Erdmann ◽  
Pedro L. Arrechea ◽  
Leron Perez ◽  
Charles Dausse ◽  
...  
Keyword(s):  
Real Time ◽  
Process Monitoring ◽  
Continuous Flow ◽  
Time Process ◽  
Flow Reactors ◽  
Segmented Polyurethanes ◽  
Continuous Flow Reactors

scholarly journals Performance of 2 Polymerization Protocols Targeting Cloned Toxoplasma Parasites

2018 ◽  
Vol 6 (9) ◽  
pp. 1577-1580
Author(s):  
Nihal A. Hanafy ◽  
Mohamed S. Badr ◽  
Ghada M. Nasr
Keyword(s):  
Toxoplasma Gondii ◽  
Real Time ◽  
Parasitic Infection ◽  
Clinical Samples ◽  
Molecular Assays ◽  
Low Concentrations ◽  
Target Dna ◽  
Primer Sets ◽  
Amplification Failure ◽  
Reference Samples

BACKGROUND: Toxoplasma gondii is a common parasitic infection of humans. Infection is usually mild. Serious complications can occur in pregnant and immunocompromised patients. AIM: The present study aims to investigate the performance of 2 different PCR protocols; real-time quantitative molecular assays (qPCR) and conventional molecular assays (cPCR), using 2 different sets of primers and by using cloned purified Toxoplasma genomic substances to be evaluated as reference samples. METHODS: The target DNA was provided in 8 different quantities. RESULTS: Amplification failure was reported only with the cPCR in samples of low concentrations using both primer sets. Quantitative PCR detected the 8 different dilutions of the purified Toxoplasma gondii using the 2 sets of primers while cPCR was sensitive to detect only 6 different dilutions. CONCLUSION: Generally real-time quantitative molecular assays, is easy to use method compared to conventional PCR assay and produces more reliable results within only one hour time but still the possible application of qPCRs in routine diagnosis necessitates analysis of a large number of clinical samples in further studies to make the proper choice.

scholarly journals Tissue specific peculiarities of vibration-induced hypoxia of the rabbit heart, liver and kidney

2016 ◽  
Vol 14 (1) ◽  
pp. 46-62 ◽  
Author(s):  
Viktoriya V Vorobieva ◽  
Petr D Shabanov
Keyword(s):  
Succinic Acid ◽  
Energy Production ◽  
Pharmacological Action ◽  
Rabbit Heart ◽  
Tissue Type ◽  
Metabolic States ◽  
Before And After ◽  
Liver And Kidney ◽  
Endogenous Substrates

The purpose of the paper was experimental study of activity of energy production of the heart, liver and kidney after harmful action of general vibration with 8 and 44 Hz frequency. The functional state of native mitochondria in tissue homogenates was studied by polarographic method by means of closed oxygen device of halvanic type in thermostated cuvette of 1 ml volume in the salt medium of incubation. Metabolic states of mitochondria of the rabbit heart, liver and kidney were modeled in vitro in oxidation of endogenous substrates (before and after administration of inhibitors of different stages of breath chain) varying exogenous substrates (before and after administration of 2.4-DNP into the cell). In order to synchronize the changes in short time, the incomplete cycle of metabolic states “endogenous breath → rest → activity” was used. The velocity of mitochondrial oxidation of endogenous substrates was determined by tissue type, and was 16.3 ± 4.3, 5.2 ± 0.6 and 8.13 ± 1.4 ng-atom О min-1mg-1 protein for the heart, liver and kidney of intact animals respectively. In the heart, after high frequent vibration, the reduction of oxidation velocity of NAD-dependent substrates in rest and in active metabolic state of mitochondria was 43 % (р ≤ 0.05) and 30 % (р ≤ 0.01) respectively, while the velocity of oxidation for endogenous succinic acid increased by 77 % (р ≤ 0.05) to 21st session of vibration, then constantly decreasing to the end of vibration sessions. The same changes but in less degree were registerted in the liver and kidney. The systems of energy production of the heart and the studied parenchimatic organs were involved in reaction on vibration exposure and reacted typically by low energetic shift with hyperactivation of endogenous succinic acid system of oxidation and inhibition of NAD-depended part of the breath chain of mitochondria. Therefore, the study of bioenergetics mechanisms of hypoxia in different tissues allows to clear the molecular targets for pharmacological action by means of substrate antihypoxants.

The effect of liver donors’ age, gender and metabolic state on pancreatic lineage activation

2021 ◽  
Vol 16 (1) ◽  
pp. 19-31
Author(s):  
Ioan V Matei ◽  
Irit Meivar-Levy ◽  
Daniela Lixandru ◽  
Simona Dima ◽  
Ioana R Florea ◽  
...  
Keyword(s):  
Replacement Therapy ◽  
Human Liver ◽  
Liver Cells ◽  
Diabetic Patients ◽  
Metabolic State ◽  
Human Liver Cells ◽  
Metabolic States ◽  
Different Ages ◽  
Liver Donors

Autologous cells replacement therapy by liver to pancreas transdifferentiation (TD) allows diabetic patients to be also the donors of their own therapeutic tissue. Aim: To analyze whether the efficiency of the process is affected by liver donors’ heterogeneity with regard to age, gender and the metabolic state. Materials & methods: TD of liver cells derived from nondiabetic and diabetic donors at different ages was characterized at molecular and cellular levels, in vitro. Results: Neither liver cells proliferation nor the propagated cells TD efficiency directly correlate with the age (3–60 years), gender or the metabolic state of the donors. Conclusion: Human liver cells derived from a wide array of ages and metabolic states can be used for autologous cells therapies for diabetics.

Integrating Thermal Sensors in a Microplate Format: Simultaneous Real-Time Quantification of Cell Number and Metabolic Activity

2022 ◽  
Author(s):  
Gilles Oudebrouckx ◽  
Juul Goossens ◽  
Seppe Bormans ◽  
Thijs Vandenryt ◽  
Patrick Wagner ◽  
...  
Keyword(s):  
Real Time ◽  
Metabolic Activity ◽  
Cell Number ◽  
Thermal Sensors
Sign in / Sign up

Export Citation Format

Share Document