scholarly journals Grain Protein Content QTLs Identified in a Durum × Wild Emmer Wheat Mapping Population Tested in Five Environments

2019 ◽  
Author(s):  
Andrii Fatiukha ◽  
Itamar Lupo ◽  
Gabriel Lidzbarsky ◽  
Valentina Klymiuk ◽  
Abraham B. Korol ◽  
...  
Keyword(s):  
Protein Content ◽  
Genetic Map ◽  
Grain Protein Content ◽  
High Density ◽  
Wild Emmer Wheat ◽  
Emmer Wheat ◽  
Wild Emmer ◽  
Grain Protein ◽  
Genetic Dissection ◽  
Ril Population

AbstractWild emmer wheat (Triticum turgidumssp.dicoccoides, WEW) was shown to exhibit high grain protein content (GPC) and therefore, possess a great potential for improvement of cultivated wheat nutritional value. A recombinant inbred line (RIL) population derived from a cross betweenT. durumvar. Svevo and WEW acc. Y12-3 was used for construction of a high-density genetic map and genetic dissection of GPC. Genotyping of 208 F6RILs with 15K wheat SNP array yielded 4,166 polymorphic SNP markers, of which 1,510 were designated as skeleton markers. A total map length of 2,169 cM was obtained with an average distance of 1.5 cM between SNPs. A total of 12 GPC QTLs with LOD score range of 2.7-35.9, and PEV of 2.6-26.6% were identified under five environments. Major QTLs with favorable alleles from WEW were identified on chromosomes 4BS, 5AS, 6BS and 7BL. The QTL region on 6BS coincided with the physical position of the previously cloned QTL,Gpc-B1. Comparisons of the physical intervals of the GPC QTLs described here with the results previously reported in other durum×WEW RIL population led to the identification of four common and two homoeologous QTLs. Exploration of the large genetic variation within WEW accessions is a precondition for discovery of exotic beneficial alleles, as we have demonstrated here, by the identification of seven novel GPC QTLs. Therefore, our research emphasizes the importance of GPC QTL dissection in diverse WEW accessions as a source of novel alleles for improvement of GPC in cultivated wheat.Key messageGenetic dissection of GPC in tetraploid durum × WEW RIL population, based on high-density SNP genetic map, revealed 12 QTLs, with favorable WEW allele for 11 QTLs.

scholarly journals A High-Density Genetic Map of Wild Emmer Wheat from the Karaca Dağ Region Provides New Evidence on the Structure and Evolution of Wheat Chromosomes

2017 ◽  
Vol 8 ◽  
Author(s):  
Chad Jorgensen ◽  
Ming-Cheng Luo ◽  
Ramesh Ramasamy ◽  
Mathew Dawson ◽  
Bikram S. Gill ◽  
...  
Keyword(s):  
Genetic Map ◽  
High Density ◽  
Wild Emmer Wheat ◽  
Emmer Wheat ◽  
Wild Emmer ◽  
New Evidence

High-density genetic map of durum wheat × wild emmer wheat based on SSR and DArT markers

2008 ◽  
Vol 117 (1) ◽  
pp. 103-115 ◽  
Author(s):  
Zvi Peleg ◽  
Yehoshua Saranga ◽  
Tatiana Suprunova ◽  
Yefim Ronin ◽  
Marion S. Röder ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Genetic Map ◽  
High Density ◽  
Wild Emmer Wheat ◽  
Emmer Wheat ◽  
Wild Emmer ◽  
Dart Markers

scholarly journals Genome-Wide Identification of QTLs for Grain Protein Content Based on Genotyping-by-Resequencing and Verification of qGPC1-1 in Rice

2020 ◽  
Vol 21 (2) ◽  
pp. 408 ◽  
Author(s):  
Yi-Bo Wu ◽  
Guan Li ◽  
Yu-Jun Zhu ◽  
Yi-Chen Cheng ◽  
Jin-Yu Yang ◽  
...  
Keyword(s):  
Protein Content ◽  
Near Infrared ◽  
Correlation Coefficients ◽  
Grain Protein Content ◽  
Chromosome 1 ◽  
Grain Protein ◽  
Rice Grain ◽  
Near Infrared Reflectance ◽  
Ril Population ◽  
Density Map

To clarify the genetic mechanism underlying grain protein content (GPC) and to improve rice grain qualities, the mapping and cloning of quantitative trait loci (QTLs) controlling the natural variation of GPC are very important. Based on genotyping-by-resequencing, a total of 14 QTLs were detected with the Huanghuazhan/Jizi1560 (HHZ/JZ1560) recombinant inbred line (RIL) population in 2016 and 2017. Seven of the fourteen QTLs were repeatedly identified across two years. Using three residual heterozygote-derived populations, a stably inherited QTL named as qGPC1-1 was validated and delimited to a ~862 kb marker interval JD1006–JD1075 on the short arm of chromosome 1. Comparing the GPC values of the RIL population determined by near infrared reflectance spectroscopy (NIRS) and Kjeldahl nitrogen determination (KND) methods, high correlation coefficients (0.966 and 0.983) were observed in 2016 and 2017. Furthermore, 12 of the 14 QTLs were identically identified with the GPC measured by the two methods. These results indicated that instead of the traditional KND method, the rapid and easy-to-operate NIRS was suitable for analyzing a massive number of samples in mapping and cloning QTLs for GPC. Using the gel-based low-density map consisted of 208 simple sequence repeat (SSR) and insert/deletion (InDel) markers, the same number of QTLs (fourteen) were identified in the same HHZ/JZ1560 RIL population, and three QTLs were repeatedly detected across two years. More stably expressed QTLs were identified based on the genome resequencing, which might be attributed to the high-density map, increasing the detection power of minor QTLs. Our results are helpful in dissecting the genetic basis of GPC and improving rice grain qualities through molecular assisted selection.

High-yielding lines of wheat carrying Gpc-B1 adapted to Mediterranean-type environments of the south and west of Australia

2014 ◽  
Vol 65 (9) ◽  
pp. 854 ◽  
Author(s):  
H. A. Eagles ◽  
Robyn McLean ◽  
R. F. Eastwood ◽  
M.-J. Appelbee ◽  
Karen Cane ◽  
...  
Keyword(s):  
Grain Yield ◽  
Protein Content ◽  
Heading Date ◽  
Grain Protein Content ◽  
Grain Weight ◽  
Wild Emmer ◽  
Grain Protein ◽  
The South ◽  
B1 Gene ◽  
Elite Cultivar

The Gpc-B1 gene from wild emmer has been proposed as a potential mechanism for improving grain protein in bread wheat without reducing grain yield. Near-isolines with and without the Gpc-B1 gene in three Australian-adapted genetic backgrounds, Gladius, Wyalkatchem and VR1128, were compared in 14 experiments across the south and west of Australia for grain yield, grain protein content and grain weight. The donor parents of Gpc-B1 were the Canadian cultivars Burnside and Somerset. One of the 14 experiments was discarded because of inadequate rust control and confounding effects of Yr36, a gene closely linked to Gpc-B1. Heading date and test weight were measured in five experiments. Across all comparisons, Gpc-B1 increased grain protein content and reduced grain weight, with a negligible effect on grain yield. Selected lines containing Gpc-B1 in a Wyalkatchem background had comparable grain yields to the elite cultivar Mace, but with significantly higher grain protein contents, slightly higher grain weights, similar heading dates and acceptable test weights. The development of agronomically acceptable lines containing Gpc-B1 was partially attributed to the removal of undesirable genes from wild emmer during the breeding of the Canadian donor parents and the use of Australian recurrent parents with high test weights.

Ultra-dense genetic map of durum wheat × wild emmer wheat developed using the 90K iSelect SNP genotyping assay

2014 ◽  
Vol 34 (4) ◽  
pp. 1549-1562 ◽  
Author(s):  
Raz Avni ◽  
Moran Nave ◽  
Tamar Eilam ◽  
Hanan Sela ◽  
Chingiz Alekperov ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Genetic Map ◽  
Snp Genotyping ◽  
Wild Emmer Wheat ◽  
Emmer Wheat ◽  
Wild Emmer ◽  
Genotyping Assay
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