mRNA adenosine methylase (MTA) deposits m6A on pri-miRNAs to modulate miRNA biogenesis in Arabidopsis thaliana

Mapping Intimacies ◽

10.1101/557900 ◽

2019 ◽

Cited By ~ 6

Author(s):

Susheel Sagar Bhat ◽

Dawid Bielewicz ◽

Natalia Grzelak ◽

Tomasz Gulanicz ◽

Zsuzsanna Bodi ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Mirna Biogenesis ◽

Related Protein ◽

Metabolic Processes ◽

Rna Pol Ii ◽

Pol Ii ◽

Transcriptional Process ◽

Novel Protein

Abstractm6A, one of the most abundant mRNA modifications, has been associated with various metabolic processes in plants. Here we show that m6A also plays a role in miRNA biogenesis in Arabidopsis thaliana. Significant reductions in plant m6A/MTA levels results in lower accumulation of miRNAs whereas pri-miRNA levels tend to be higher in such plants. m6A-IP Seq and MTA-GFP RIP were used to show that many pri-miRNAs are m6A methylated and are bound by MTA, further demonstrating that pri-miRNAs can also be substrates for m6A methylation by MTA. We report that MTA interacts with RNA Pol II, supporting the assumption that m6A methylation is a co-transcriptional process, and also identify TGH, a known miRNA biogenesis related protein, as a novel protein that interacts with MTA. Finally, reduced levels of miR393b may partially explain the strong auxin insensitivity seen in Arabidopsis plants with reduced m6A levels.

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Intronic microRNAs: a crossroad in gene regulation

Biochemical Society Transactions ◽

10.1042/bst20120023 ◽

2012 ◽

Vol 40 (4) ◽

pp. 759-761 ◽

Cited By ~ 23

Author(s):

Natalia Gromak

Keyword(s):

Gene Regulation ◽

Human Gene ◽

Mrna Splicing ◽

Translational Repression ◽

Mirna Biogenesis ◽

Mrna Transcript ◽

Rna Pol Ii ◽

Pol Ii ◽

Human Genes ◽

Non Coding Rnas

Most human genes transcribed by RNA Pol II (polymerase II) contain short exons separated by long tracts of non-coding intronic sequences. In addition to their role in generating proteomic diversity through the process of alternative splicing, intronic sequences host many ncRNAs (non-coding RNAs), involved in various gene regulation processes. miRNAs (microRNAs) are short ncRNAs that mediate either mRNA transcript translational repression and/or degradation. Between 50 and 80% of miRNAs are encoded within introns of host mRNA genes. This observation suggests that there is co-regulation between the miRNA biogenesis and pre-mRNA splicing processes. The present review summarizes current advances in this field and discusses possible roles for intronic co-transcriptional cleavage events in the regulation of human gene expression.

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Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana

Nucleic Acids Research ◽

10.1093/nar/gkz526 ◽

2019 ◽

Vol 47 (15) ◽

pp. 7886-7900 ◽

Cited By ~ 3

Author(s):

Junli Wang ◽

Susu Chen ◽

Ning Jiang ◽

Ning Li ◽

Xiaoyan Wang ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Alternative Splicing ◽

Rna Polymerase ◽

Rna Polymerase Ii ◽

Promoter Activity ◽

Mirna Biogenesis ◽

Stable Complex ◽

Transcriptional Elongation ◽

Related Protein

Abstract The intron-lariat spliceosome (ILS) complex is highly conserved among eukaryotes, and its disassembly marks the end of a canonical splicing cycle. In this study, we show that two conserved disassembly factors of the ILS complex, Increased Level of Polyploidy1-1D (ILP1) and NTC-Related protein 1 (NTR1), positively regulate microRNA (miRNA) biogenesis by facilitating transcriptional elongation of MIRNA (MIR) genes in Arabidopsis thaliana. ILP1 and NTR1 formed a stable complex and co-regulated alternative splicing of more than a hundred genes across the Arabidopsis genome, including some primary transcripts of miRNAs (pri-miRNAs). Intriguingly, pri-miRNAs, regardless of having introns or not, were globally down-regulated when the ILP1 or NTR1 function was compromised. ILP1 and NTR1 interacted with core miRNA processing proteins Dicer-like 1 and Serrate, and were required for proper RNA polymerase II occupancy at elongated regions of MIR chromatin, without affecting either MIR promoter activity or pri-miRNA decay. Our results provide further insights into the regulatory role of spliceosomal machineries in the biogenesis of miRNAs.

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