scholarly journals Ribosomal DNA and the rDNA-binding protein Indra mediate non-random sister chromatid segregation in Drosophila male germline stem cells

2018 ◽  
Author(s):  
George Watase ◽  
Yukiko Yamashita
Keyword(s):  
Stem Cells ◽  
Ribosomal Dna ◽  
Sister Chromatid ◽  
Zinc Finger Protein ◽  
Biological Significance ◽  
Germline Stem Cells ◽  
Male Germline ◽  
Chromatid Segregation ◽  
Male Germline Stem Cells ◽  
Rdna Copy

Although non-random sister chromatid segregation has been proposed to underlie asymmetric cell divisions, the underlying biological significance or mechanisms remained elusive. Here we show that non-random sister chromatid segregation during asymmetric division of Drosophila male germline stem cells is mediated by ribosomal DNA (rDNA) loci, consisting of hundreds of tandemly repeated rDNA units. We identify a novel zinc-finger protein CG2199/Indra that binds to rDNA and control non-random sister chromatid segregation. Our data indicate that non-random sister chromatid segregation may reflect the segregation of sister chromatids with different rDNA copy numbers after unequal sister chromatid exchange to maintain rDNA copy number through generations. To our knowledge, this is the first study to provide mechanistic insights into the mechanism of non-random sister chromatid segregation.

scholarly journals Author response: Transgenerational dynamics of rDNA copy number in Drosophila male germline stem cells

2017 ◽  
Author(s):  
Kevin L Lu ◽  
Jonathan O Nelson ◽  
George J Watase ◽  
Natalie Warsinger-Pepe ◽  
Yukiko M Yamashita
Keyword(s):  
Stem Cells ◽  
Copy Number ◽  
Author Response ◽  
Germline Stem Cells ◽  
Male Germline ◽  
Male Germline Stem Cells ◽  
Rdna Copy ◽  
Rdna Copy Number

Functions of promyelocytic leukaemia zinc finger (Plzf) in male germline stem cell development and differentiation

2019 ◽  
Vol 31 (8) ◽  
pp. 1315 ◽  
Author(s):  
Daguia Zambe John Clotaire ◽  
Yudong Wei ◽  
Xiuwei Yu ◽  
Tamgue Ousman ◽  
Jinlian Hua
Keyword(s):  
Stem Cells ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Development Stage ◽  
Cell Development ◽  
Promyelocytic Leukaemia ◽  
Germline Stem Cell ◽  
Germline Stem Cells ◽  
Male Germline ◽  
Finger Protein

Promyelocytic leukaemia zinc finger (Plzf), also known as zinc finger and BTB domain containing 16 (ZBTB16) or zinc-finger protein 145 (ZFP145), is a critical zinc finger protein of male germline stem cells (mGSCs). Multiple lines of evidence indicate that Plzf has a central role in the development, differentiation and maintenance of many stem cells, including mGSCs, and Plzf has been validated as an essential transcription factor for mammalian testis development and spermatogenesis. This review summarises current literature focusing on the significance of Plzf in maintaining and regulating self-renewal and differentiation of mGSCs, especially goat mGSCs. The review summarises evidence of the specificity of Plzf expression in germ cell development stage, the known functions of Plzf and the microRNA-mediated mechanisms that control Plzf expression in mGSCs.

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