scholarly journals Mouse Academy: high-throughput automated training and trial-by-trial behavioral analysis during learning

2018 ◽  
Author(s):  
Mu Qiao ◽  
Tony Zhang ◽  
Cristina Segalin ◽  
Sarah Sam ◽  
Pietro Perona ◽  
...  

ABSTRACTProgress in understanding how individual animals learn will require high-throughput standardized methods for behavioral training but also advances in the analysis of the resulting behavioral data. In the course of training with multiple trials, an animal may change its behavior abruptly, and capturing such events calls for a trial-by-trial analysis of the animal’s strategy. To address this challenge, we developed an integrated platform for automated animal training and analysis of behavioral data. A low-cost and space-efficient apparatus serves to train entire cohorts of mice on a decision-making task under identical conditions. A generalized linear model (GLM) analyzes each animal’s performance at single-trial resolution. This model infers the momentary decision-making strategy and can predict the animal’s choice on each trial with an accuracy of ~80%. We also assess the animal’s detailed trajectories and body poses within the apparatus. Unsupervised analysis of these features revealed unusual trajectories that represent hesitation in the response. This integrated hardware/software platform promises to accelerate the understanding of animal learning.

Entropy ◽  
2021 ◽  
Vol 23 (6) ◽  
pp. 727
Author(s):  
Eric J. Ma ◽  
Arkadij Kummer

We present a case study applying hierarchical Bayesian estimation on high-throughput protein melting-point data measured across the tree of life. We show that the model is able to impute reasonable melting temperatures even in the face of unreasonably noisy data. Additionally, we demonstrate how to use the variance in melting-temperature posterior-distribution estimates to enable principled decision-making in common high-throughput measurement tasks, and contrast the decision-making workflow against simple maximum-likelihood curve-fitting. We conclude with a discussion of the relative merits of each workflow.


2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Woo Seok Kim ◽  
Sungcheol Hong ◽  
Milenka Gamero ◽  
Vivekanand Jeevakumar ◽  
Clay M. Smithhart ◽  
...  

AbstractThe vagus nerve supports diverse autonomic functions and behaviors important for health and survival. To understand how specific components of the vagus contribute to behaviors and long-term physiological effects, it is critical to modulate their activity with anatomical specificity in awake, freely behaving conditions using reliable methods. Here, we introduce an organ-specific scalable, multimodal, wireless optoelectronic device for precise and chronic optogenetic manipulations in vivo. When combined with an advanced, coil-antenna system and a multiplexing strategy for powering 8 individual homecages using a single RF transmitter, the proposed wireless telemetry enables low cost, high-throughput, and precise functional mapping of peripheral neural circuits, including long-term behavioral and physiological measurements. Deployment of these technologies reveals an unexpected role for stomach, non-stretch vagal sensory fibers in suppressing appetite and demonstrates the durability of the miniature wireless device inside harsh gastric conditions.


2021 ◽  
pp. 009614422198997
Author(s):  
Marianna Charitonidou

The article presents the reasons for which the issue of providing housing to low-income citizens has been a real challenge in Addis Ababa during the recent years and will continue to be, given that its population is growing extremely fast. It examines the tensions between the universal aspirations and the local realities in the case of some of Ethiopia’s most ambitious mass pro-poor housing schemes, such as the “Addis Ababa Grand Housing Program” (AAGHP), which was launched in 2004 and was integrated in the “Integrated Housing Development Program” (IHDP) in 2006. The article argues that the quotidian practices of communities and their socio-economic and cultural characteristics are related to the spatial attributes of co-housing practices. Drawing upon the idea that there is a mutual correspondence between social and spatial structures, it places particular emphasis on the analysis of the IHDP and aims to show that to shape strategies that take into account the social and cultural aspects of daily life of the poor citizens of Addis Ababa, it is pivotal to invite them to take part in the decision-making processes regarding their resettlement. Departing from the fact that a large percentage of the housing supply in Addis Ababa consists of informal unplanned housing, the article also compares the commoning practices in kebele houses and condominium units. The former refers to the legal informal housing units owned by the government and rented to their dwellers, whereas the latter concerns the housing blocks built in the framework of the IHDP for the resettlement of the kebele dwellers. The article analyzes these processes of resettlement, shedding light of the fact that kebele houses were located at the inner city, whereas the condominiums are located in the suburbs. Despite the fact that the living conditions in the condominium units are of a much higher quality than those in the kebele houses, their design underestimated or even neglected the role of the commoning practices. The article highlights the advantages of commoning practices in architecture and urban planning, and how the implementation of participation-oriented solutions can respond to the difficulties of providing housing. It argues that understanding the significance of the endeavors that take into account the opinions of dwellers during the phase of decision-making goes hand in hand with considering commoning practices as a source of architecture and urban planning frameworks for low-cost housing in this specific context. The key argument of the article is that urban planning and architecture solutions in Addis Ababa should be based on the principles of the so-called “negotiated planning” approach, which implies a close analysis of the interconnections between planning, infrastructure, and land.


Materials ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 2910
Author(s):  
Chaoyi Ding ◽  
Chun Liu ◽  
Ligang Zhang ◽  
Di Wu ◽  
Libin Liu

The high cost of development and raw materials have been obstacles to the widespread use of titanium alloys. In the present study, the high-throughput experimental method of diffusion couple combined with CALPHAD calculation was used to design and prepare the low-cost and high-strength Ti-Al-Cr system titanium alloy. The results showed that ultra-fine α phase was obtained in Ti-6Al-10.9Cr alloy designed through the pseudo-spinodal mechanism, and it has a high yield strength of 1437 ± 7 MPa. Furthermore, application of the 3D strength model of Ti-6Al-xCr alloy showed that the strength of the alloy depended on the volume fraction and thickness of the α phase. The large number of α/β interfaces produced by ultra-fine α phase greatly improved the strength of the alloy but limited its ductility. Thus, we have demonstrated that the pseudo-spinodal mechanism combined with high-throughput diffusion couple technology and CALPHAD was an efficient method to design low-cost and high-strength titanium alloys.


NeuroImage ◽  
2011 ◽  
Vol 54 (2) ◽  
pp. 824-835 ◽  
Author(s):  
N. Novitskiy ◽  
J.R. Ramautar ◽  
K. Vanderperren ◽  
M. De Vos ◽  
M. Mennes ◽  
...  

2007 ◽  
Vol 28 (7) ◽  
pp. 602-613 ◽  
Author(s):  
Christian-G. Bénar ◽  
Daniele Schön ◽  
Stephan Grimault ◽  
Bruno Nazarian ◽  
Boris Burle ◽  
...  

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