scholarly journals Trait-based approach to bacterial growth efficiency

2018 ◽  
Author(s):  
Mario E. Muscarella ◽  
Xia Meng Howey ◽  
Jay T. Lennon
Keyword(s):  
Growth Rate ◽  
Bacterial Growth ◽  
Maximum Growth ◽  
Growth Efficiency ◽  
Maximum Growth Rate ◽  
Bacterial Growth Efficiency ◽  
Species Identity ◽  
Trade Offs ◽  
Aggregate Property ◽  
Resource Type

AbstractBacterial growth efficiency (BGE) is the proportion of assimilated carbon that is converted into biomass and reflects the balance between growth and energetic demands. Often measured as an aggregate property of the community, BGE is highly variable within and across ecosystems. To understand this variation, we first identified how species identity and resource type affect BGE using 20 bacterial isolates belonging to the phylum Proteobacteria that were enriched from north temperate lakes. Using a trait-based approach that incorporated genomic and phenotypic information, we characterized the metabolism of each isolate and tested for predicted trade-offs between growth rate and efficiency. A substantial amount of variation in BGE could be explained at both broad (i.e., order, 20 %) and fine (i.e., strain, 58 %) taxonomic levels. While resource type was a relatively weak predictor across species, it explained > 60 % of the variation in BGE within a given species. Furthermore, a metabolic trade-off (between maximum growth rate and efficiency) and genomic features revealed that BGE is a predictable metabolic feature. Our study suggests that genomic and phylogenetic information may help predict aggregate microbial community functions like BGE and the fate of carbon in ecosystems.Originality and SignificanceBacterial growth efficiency (BGE) is an important yet notoriously variable measure of metabolism that has proven difficult to predict. To better understand how assimilated carbon is allocated, we explored growth efficiency across a collection of bacteria strains using a trait-based approach. Specifically, we measured respiration and biomass formation rates for populations grown in minimal media containing one of three carbon resources. In addition, we collected a suite of physiological traits to describe each strain, and we sequenced the genome of each organism. Our results suggest that species identity and resource type may contribute to growth efficiency when measured as an aggregate property of a natural community. In addition, we identified genomic pathways that are associated with elevated BGE. The findings have implications for integrating microbial metabolism from the cellular to ecosystem scale.

scholarly journals Size evolution in microorganisms masks trade-offs predicted by the growth rate hypothesis

2016 ◽  
Vol 283 (1845) ◽  
pp. 20162272 ◽  
Author(s):  
Isabelle Gounand ◽  
Tanguy Daufresne ◽  
Dominique Gravel ◽  
Corinne Bouvier ◽  
Thierry Bouvier ◽  
...  
Keyword(s):  
Growth Rate ◽  
Cell Size ◽  
Competitive Ability ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Strong Positive Correlation ◽  
Competitive Strategies ◽  
Growth Rate Hypothesis ◽  
Trade Offs ◽  
Size Evolution

Adaptation to local resource availability depends on responses in growth rate and nutrient acquisition. The growth rate hypothesis (GRH) suggests that growing fast should impair competitive abilities for phosphorus and nitrogen due to high demand for biosynthesis. However, in microorganisms, size influences both growth and uptake rates, which may mask trade-offs and instead generate a positive relationship between these traits (size hypothesis, SH). Here, we evolved a gradient of maximum growth rate ( μ max ) from a single bacterium ancestor to test the relationship among μ max , competitive ability for nutrients and cell size, while controlling for evolutionary history. We found a strong positive correlation between μ max and competitive ability for phosphorus, associated with a trade-off between μ max and cell size: strains selected for high μ max were smaller and better competitors for phosphorus. Our results strongly support the SH, while the trade-offs expected under GRH were not apparent. Beyond plasticity, unicellular populations can respond rapidly to selection pressure through joint evolution of their size and maximum growth rate. Our study stresses that physiological links between these traits tightly shape the evolution of competitive strategies.

scholarly journals Heterotrophic Bacterial Growth Efficiency and Community Structure at Different Natural Organic Carbon Concentrations

2003 ◽  
Vol 69 (7) ◽  
pp. 3701-3709 ◽  
Author(s):  
Alexander Eiler ◽  
Silke Langenheder ◽  
Stefan Bertilsson ◽  
Lars J. Tranvik
Keyword(s):  
Growth Rate ◽  
Organic Matter ◽  
Organic Carbon ◽  
Dissolved Organic Matter ◽  
Community Composition ◽  
Bacterial Growth ◽  
Bacterial Biomass ◽  
Growth Efficiency ◽  
Bacterial Growth Efficiency ◽  
Doc Concentration

ABSTRACT Batch cultures of aquatic bacteria and dissolved organic matter were used to examine the impact of carbon source concentration on bacterial growth, biomass, growth efficiency, and community composition. An aged concentrate of dissolved organic matter from a humic lake was diluted with organic compound-free artificial lake water to obtain concentrations of dissolved organic carbon (DOC) ranging from 0.04 to 2.53 mM. The bacterial biomass produced in the cultures increased linearly with the DOC concentration, indicating that bacterial biomass production was limited by the supply of carbon. The bacterial growth rate in the exponential growth phase exhibited a hyperbolic response to the DOC concentration, suggesting that the maximum growth rate was constrained by the substrate concentration at low DOC concentrations. Likewise, the bacterial growth efficiency calculated from the production of biomass and CO2 increased asymptotically from 0.4 to 10.4% with increasing DOC concentration. The compositions of the microbial communities that emerged in the cultures were assessed by separation of PCR-amplified 16S rRNA fragments by denaturing gradient gel electrophoresis. Nonmetric multidimensional scaling of the gel profiles showed that there was a gradual change in the community composition along the DOC gradient; members of the β subclass of the class Proteobacteria and members of the Cytophaga-Flavobacterium group were well represented at all concentrations, whereas members of the α subclass of the Proteobacteria were found exclusively at the lowest carbon concentration. The shift in community composition along the DOC gradient was similar to the patterns of growth efficiency and growth rate. The results suggest that the bacterial growth efficiencies, the rates of bacterial growth, and the compositions of bacterial communities are not constrained by substrate concentrations in most natural waters, with the possible exception of the most oligotrophic environments.

The effect of energy and nitrogen supply pattern on rumen bacterial growthin vitro

1991 ◽  
Vol 53 (2) ◽  
pp. 165-175 ◽  
Author(s):  
P. H. Henning ◽  
D. G. Steyn ◽  
H. H. Meissner
Keyword(s):  
Bacterial Growth ◽  
Specific Growth ◽  
Growth Efficiency ◽  
Bacterial Growth Efficiency ◽  
Batch Cultures ◽  
Time Zero ◽  
N Supply ◽  
Pulse Dose ◽  
Supply Pattern ◽  
Glucose Supply

AbstractThe effect of energy and nitrogen (N) supply pattern on rumen bacterial growth was investigated in vitro. In experiment 1, glucose was was fed to batch cultures of mixed rumen bacteria according to three patterns namely a pulse dose at time zero (P); even increments at 0·5-h intervals (G) or an intermediate pattern (I), whilst N was supplied in excess. In experiment 2, glucose and N (not in excess) were fed to batch cultures according to four patterns namely glucose and N as pulse doses at time zero, (EPNP); glucose as a pulse dose at time zero and N in 24 even increments at 0·5-h intervals (EPNG); glucose in 24 even increments at 0·5-h intervals and N as a pulse dose at time zero (EGNP) or both glucose and N in 24 even increments at 0·5-h intervals (EGNG). Fermentaton was studied over a 12-h period for both experiments.In experiment 1, bacterial growth efficiency and specific growth rate (39·8,35·5 and 29·9 (g bacterial dry matter (DM) per mol glucose utilized) and 0·33, 0·27 and 0·20 (fraction per h) for treatments P, I, and G respectively) differed significantly between glucose supply patterns. In experiment 2, bacterial growth efficiency and specific growth rate (33·8, 34·7, 25·9 and 22·5 (g baterial DM per mol glucose) and 0·21, 0·18, 0·14 and 0·13 (fraction per h) for treatments EPNP, EPNG, EGNP and EGNG respectively) differed significantly only between glucose supply patterns.It is concluded that the pattern according to which a given amount of energy becomes available affects bacterial growth efficiency, with the fastest supply rate giving the highest efficiency and that, within accepted levels of N supply, synchronization between energy and N availability may be of less importance to bacterial growth efficiency than the energy supply pattern.

A note on growth curves of rabbit lines selected on growth rate or litter size

1993 ◽  
Vol 57 (2) ◽  
pp. 332-334 ◽  
Author(s):  
A. Blasco ◽  
E. Gómez
Keyword(s):  
Growth Rate ◽  
Litter Size ◽  
Growth Curves ◽  
Live Weight ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Standard Errors ◽  
Adult Weight ◽  
Weight Growth ◽  
Using Data

Two synthetic lines of rabbits were used in the experiment. Line V, selected on litter size, and line R, selected on growth rate. Ninety-six animals were randomly collected from 48 litters, taking a male and a female each time. Richards and Gompertz growth curves were fitted. Sexual dimorphism appeared in the line V but not in the R. Values for b and k were similar in all curves. Maximum growth rate took place in weeks 7 to 8. A break due to weaning could be observed in weeks 4 to 5. Although there is a remarkable similarity of the values of all the parameters using data from the first 20 weeks only, the higher standard errors on adult weight would make 30 weeks the preferable time to take data for live-weight growth curves.

Reassessment of Maximum Growth Rates for C3 and C4 Crops

1978 ◽  
Vol 14 (1) ◽  
pp. 1-5 ◽  
Author(s):  
J. L. Monteith
Keyword(s):  
Growth Rate ◽  
Growth Rates ◽  
Growing Season ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Crop Growth ◽  
Close Inspection ◽  
Similar Difference ◽  
Photosynthetic Efficiencies

SUMMARYFigures for maximum crop growth rates, reviewed by Gifford (1974), suggest that the productivity of C3 and C4 species is almost indistinguishable. However, close inspection of these figures at source and correspondence with several authors revealed a number of errors. When all unreliable figures were discarded, the maximum growth rate for C3 stands fell in the range 34–39 g m−2 d−1 compared with 50–54 g m−2 d−1 for C4 stands. Maximum growth rates averaged over the whole growing season showed a similar difference: 13 g m−2 d−1 for C3 and 22 g m−2 d−1 for C4. These figures correspond to photosynthetic efficiencies of approximately 1·4 and 2·0%.

Interpretation of Experimental Data with Regard to the Activated Sludge Model No.1 and Calibration of the Model for Municipal Wastewater Treatment Plants

1992 ◽  
Vol 25 (6) ◽  
pp. 167-183 ◽  
Author(s):  
H. Siegrist ◽  
M. Tschui
Keyword(s):  
Experimental Data ◽  
Growth Rate ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Municipal Wastewater Treatment ◽  
Activated Sludge Model

The wastewater of the municipal treatment plants Zürich-Werdhölzli (350000 population equivalents), Zürich-Glatt (110000), and Wattwil (20000) have been characterized with regard to the activated sludge model Nr.1 of the IAWPRC task group. Zürich-Glatt and Wattwil are partly nitrifying treatment plants and Zürich-Werdhölzli is fully nitrifying. The mixing characteristics of the aeration tanks at Werdhölzli and Glatt were determined with sodium bromide as a tracer. The experimental data were used to calibrate hydrolysis, heterotrophic growth and nitrification. Problems arising by calibrating hydrolysis of the paniculate material and by measuring oxygen consumption of heterotrophic and nitrifying microorganisms are discussed. For hydrolysis the experimental data indicate first-order kinetics. For nitrification a maximum growth rate of 0.40±0.07 d−1, corresponding to an observed growth rate of 0.26±0.04 d−1 was calculated at 10°C. The half velocity constant found for 12 and 20°C was 2 mg NH4-N/l. The calibrated model was verified with experimental dam of me Zürich-Werdhölzli treatment plant during ammonia shock load.

Dietary Protein Requirements of Fishes — A Reassessment

1987 ◽  
Vol 44 (11) ◽  
pp. 1995-2001 ◽  
Author(s):  
Stephen H. Bowen
Keyword(s):  
Growth Rate ◽  
Dietary Protein ◽  
Protein Concentration ◽  
Trophic Ecology ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Intake Rate ◽  
Protein Requirement ◽  
Fish Physiology ◽  
Protein Retention

It is widely believed that fishes require more dietary protein than other vertebrates. Many aspects of fish physiology, nutrition, and trophic ecology have been interpreted within the context of this high protein requirement. Here, fishes are compared with terrestrial homeotherms in terms of (1) protein requirement for maintenance, (2) relative protein concentration in the diet required for maximum growth rate, (3) protein intake rate required for maximum growth rate, (4) efficiency of protein retention in growth, and (5) weight of growth achieved per weight of protein ingested. The two animal groups compared differ only in relative protein concentration in the diet required for maximum growth rate. This difference is explained in terms of homeotherms' greater requirement for energy and does not reflect absolute differences in protein requirement. The remaining measures of protein requirement suggest that fishes and terrestrial homeotherms are remarkably similar in their use of protein as a nutritional resource. Reinterpretation of the role of protein in fish physiology, nutrition, and trophic ecology is perhaps in order.

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