scholarly journals Rapidly Assessing the Quality of Targeted Proteomics Experiments Through Monitoring Stable-isotope Labeled Standards

2018 ◽  
Author(s):  
Bryson C. Gibbons ◽  
Thomas L. Fillmore ◽  
Yuqian Gao ◽  
Ronald J. Moore ◽  
Tao Liu ◽  
...  

AbstractTargeted proteomics experiments based on selected reaction monitoring (SRM) have gained wide adoption in clinical biomarker, cellular modeling and numerous other biological experiments due to their highly accurate and reproducible quantification. The quantitative accuracy in targeted proteomics experiments is reliant on the stable-isotope, heavy-labeled peptide standards which are spiked into a sample and used as a reference when calculating the abundance of endogenous peptides. Therefore, the quality of measurement for these standards is a critical factor in determining whether data acquisition was successful. With improved MS instrumentation that enables the monitoring of hundreds of peptides in hundreds to thousands of samples, quality assessment is increasingly important and cannot be performed manually. We present Q4SRM, a software tool that rapidly checks the signal from all heavy labeled peptides and flags those that fail quality control metrics. Using four metrics, the tool detects problems both with individual SRM transitions and the collective group of transitions that monitor a single peptide. The program’s speed enables its use at the point of data acquisition and can be ideally run immediately upon the completion of an LC-SRM-MS analysis.

2018 ◽  
Vol 18 (2) ◽  
pp. 694-699 ◽  
Author(s):  
Bryson C. Gibbons ◽  
Thomas L. Fillmore ◽  
Yuqian Gao ◽  
Ronald J. Moore ◽  
Tao Liu ◽  
...  

Author(s):  
Jan Schmidt ◽  
Martina Kunderova ◽  
Nela Pilbauerova ◽  
Martin Kapitan

This work provides a narrative review covering evidence-based recommendations for pericoronitis management (Part A) and a systematic review of antibiotic prescribing for pericoronitis from January 2000 to May 2021 (Part B). Part A presents the most recent, clinically significant, and evidence-based guidance for pericoronitis diagnosis and proper treatment recommending the local therapy over antibiotic prescribing, which should be reserved for severe conditions. The systematic review includes publications analyzing sets of patients treated for pericoronitis and questionnaires that identified dentists' therapeutic approaches to pericoronitis. Questionnaires among dentists revealed that almost 75% of them prescribed antibiotics for pericoronitis, and pericoronitis was among the top 4 in the frequency of antibiotic use within the surveyed diagnoses and situations. Studies involving patients showed that antibiotics were prescribed to more than half of the patients with pericoronitis, and pericoronitis was among the top 2 in the frequency of antibiotic use within the monitored diagnoses and situations. The most prescribed antibiotics for pericoronitis were amoxicillin and metronidazole. The systematic review results show abundant and unnecessary use of antibiotics for pericoronitis and are in strong contrast to evidence-based recommendations summarized in the narrative review. Adherence of dental professionals to the recommendations presented in this work can help rapidly reduce the duration of pericoronitis, prevent its complications, and reduce the use of antibiotics and thus reduce its impact on patients' quality of life, healthcare costs, and antimicrobial resistance development.


2013 ◽  
Vol 318 ◽  
pp. 572-575
Author(s):  
Li Li Yu ◽  
Yu Hong Li ◽  
Ai Feng Wang

In this paper a quality monitoring system for seismic while drilling (SWD) that integrates the whole process of data acquisition was developed. The acquisition equipment, network status and signals of accelerometer and geophone were monitored real-time. With fast signal analysis and quality evaluation, the acquisition parameters and drilling engineering parameters can be adjusted timely. The application of the system can improve the quality of data acquisition and provide subsequent processing and interpretation with high qualified reliable data.


2015 ◽  
Author(s):  
W. David Joiner ◽  
Charles J. Cook

Propulsion shaft alignment is a necessary and crucial step in the ship construction process, with manning and schedule constraints requiring accurate results as efficiently as possible. There are two methods for measuring the bearing loading along the shaft line: strain gages and load cells. The legacy method for using strain gages required a lot of man power and the legacy method for using load cells was dependent on the quality of machinist made available. Strain gages are the transducers of choice for measurement; however the data acquisition, especially for ships with long shafting systems, can require many strain gage positions and personnel to conduct tests. Load cells are used to validate the accuracy of the strain gage method and to calculate the shaft runout at each bearing location.


Author(s):  
Ezequiel Saretta ◽  
Antonio P. de Camargo ◽  
Tarlei A. Botrel ◽  
Marinaldo F. Pinto ◽  
Geancarlo T. Katsurayama ◽  
...  

ABSTRACT Current meters are equipment widely used for estimating flow velocity in rivers and streams. Periodic calibrations of current meters are important to ensure the quality of measurements, but the required testing facilities are complex and only available in a few institutions. However, advances in electronics and automation may contribute to developing simple and reliable calibration systems. Thus, this study aimed to develop an automated system for testing current meters, which consisted of a trapezoidal channel, a step motor, a tow car and a management system, composed of a supervisory application and microprocessed modules to control the motor and the data acquisition. Evaluations of the displacement velocity showed that it matched the reference value up to 1.85 m s-1 for a vertical-axis current meter and 2.3 m s-1 for a horizontal-axis one. The developed system showed reliability during tests, for both current meter movement and data acquisition. The management of the system based on the developed modules and the supervisory application improved its user interface, turning all the procedure into a simple task.


1995 ◽  
Vol 391 ◽  
Author(s):  
Larry M. Ting ◽  
G. Dixit ◽  
M. Jain ◽  
K. A. Littau ◽  
H. Tran ◽  
...  

AbstractIn this study, we have evaluated electromigration performance of W-plug vias, fabricated with process variations in via hole etching as well as in via barrier deposition, on a TiN(barrier)/AlCu/TiN metallization system. We found that via etch profile scheme as well as deposition conformity for via barrier can significantly affect W-plug via lifetimes. The results indicate that the quality of contact with AlCu layer of bottom level metal is a critical factor in determining via lifetime. Precise control of via etching or by use of conformal via barrier to ensure a good barrier between W-plug and the AlCu of bottom level metal is essential for achieving reliable via structures.


2012 ◽  
pp. 1148-1163
Author(s):  
Posy Bidwell ◽  
Stephen Thomas ◽  
Silvia Stringhini

A critical factor in addressing the human resources crisis in low and middle income countries (LMIC) is the ability to recruit, motivate, and retain health workers. Failure in this area is one of the main causes of decline in availability of services and quality of care. Various financial and non-financial incentives have been implemented and this chapter will explore available evidence to see whether they have influenced motivation. Additionally, Maslow’s hierarchy of needs is used to determine if there is a hierarchy of how incentives are valued. While Maslow’s model is a useful tool to classify themes of health worker needs, it would appear that workers are motivated without each level having to be fulfilled in turn. While financial incentives may help with retention, they can cause erosion of professional ethos, do not increase job satisfaction, or act as motivators to perform well. More research needs to be done in order to design more effective human resources strategies.


2019 ◽  
Vol 4 (2) ◽  
pp. 94-103
Author(s):  
Zulkani Sinaga ◽  
Achmad Muhazir ◽  
Muhamad Ilham Fuadi

ABSTRAKPenelitian ini dilakukan pada salah satu produsen spare part otomotif PT. XYZ memiliki permasalahan dan menjadi perhatian serius pada bagian pengelasan adanya produk cacat yang dapat menurunkan nilai dan kualitas produk sehingga banyak produk yang ditolak oleh konsumen karena tidak sesuai dengan standar, dimana banyak terdapat kotoran las (Spatter) yang menmpel pada Joint Exhaust produk Half Outer Comp merupakan bagian komponen dari knalpot sehingga mengurangi produktifitas produksi pada bagian inspeksi akhir, permasalahan di atas dianggap perlu dilakukan penelitian untuk mencari penyebab hal tersebut. Beberapa langkah dilakukan guna memperbaiki cacat yang ada yaitu langkah awal dilakukan observasi dan wawancara ke bagian produksi, metode yang digunakan untuk pengolahan data hasil observasi menggunakan metode DMAIC (Define, Measure, Analisys, Improve, Control), kemudian dilakukan optimalisasi dan modifikasi Jig Welding dengan mendesain ulang menggunakan perangkat software Solidwork versi 2018, kemudian mengubah posisi Jig sebelumnya vertikal menjadi horizontal agar supaya pada saat pengelasan Spatter tidak langsung jatuh ke Joint Exhaust dan memberikan penahan tambahan pada Jig guna mengurangi jumlah Spatter menempel pada Half Outer Comp. Pada penelitian ini diperoleh hasil dimana jumlah Spatter yang menempel pada Joint Exhaust sangat berkurang dan memperoleh hasil produk yang lebih baik sehingga meningkatkan produktivitas pada bagian inspeksi akhir sebelumnya 58,33% menjadi sebesar 83.33%.  Kata Kunci :  Optimalisasi, Spatter, DMAIC, Desain, Solidwork 2018 ABSTRACTThis research was conducted at one of the automotive spare part manufacturers PT. XYZ has a problem and is a serious concern on the welding part of the presence of defective products that can reduce the value and quality of the product so that many products are rejected by consumers because it does not comply with standards, where there is a lot of welding dirt (Spatter) which attaches to the Joint Exhaust Half Outer Comp product is a component of the muffler so as to reduce production productivity at the final inspection, the above problems are considered necessary to do research to find the cause of it. Several steps were taken to correct existing defects, namely the initial steps carried out observation and interviews to the production department, the method used for processing the observation data using the DMAIC method (Define, Measure, Analysis, Improve, Control), then optimization and modification of Jig Welding with redesign using the 2018 Solidwork software tool, then changing the position of the previous vertical jig to horizontal so that when welding Spatter does not directly fall into the Joint Exhaust and provide additional support to the Jig to reduce the number of Spatter attached to the Half Outer Comp. In this study the results obtained where the number of Spatter attached to the Joint Exhaust is greatly reduced and obtain better product results thereby increasing productivity in the previous final inspection section of 58.33% to 83.33%. Keywords : Optimization, Spatter, DMAIC, Design, Solidwork 2018


1997 ◽  
Vol 38 (1) ◽  
pp. 173-175 ◽  
Author(s):  
K. Ito ◽  
J. Kato ◽  
S. Okada ◽  
T. Kumazaki

Purpose: In three-dimensional (3-D) contrast MR angiography, temporal misregistration between the data acquisition period and the arrival of the contrast agent in the target vessels is thought to degrade the quality of the reconstructed images. The purpose of this study was to demonstrate and investigate this effect in phantom experiments. Material and Methods: MR images of a phantom tube were evaluated with flowing materials of water or Gd-DTPA solution by changing from water to Gd-DTPA solution halfway through the data acquisition period. Results: While no signal could be acquired with a stream of water in the tube, a clear signal was obtained with a flow of Gd-DTPA solution. Blurring and ghost artifacts surrounding the tube along the phase-encoding direction were observed when the flowing material was changed from water to Gd-DTPA solution halfway through the data acquisition period. Conclusion: K-space filter effect occurs during 3-D contrast MR angiography owing to the transient passage of the contrast agent, and this effect causes spatial artifacts in the reconstructed images.


2012 ◽  
Vol 562-564 ◽  
pp. 1209-1212
Author(s):  
Peng Ju Li ◽  
Huan Huan Liu ◽  
Jia Yin Wei

In order to improve the application effect of the NMR in Petroleum exploration and development, the quality of T2 spectrum inversion need to be controlled. Echo resolution matrix,T2 spectrum resolution matrix are deduced, the quality control index of T2 spectrum inversion is provided: the stretch coefficient, maximum, minimum, average and range of principal diagonal elements of resolution matrix. Calculating the inversion quality control index under different observation modes and choosing the observation mode of resolution matrix approximate to unit matrix for data acquisition can improve T2 spectrum inversion quality. Numerical experimentation indicate there is very good consistency between inversion quality and control index. Hence, it may direct the design before NMR data acquisition, control inversion quality of T2 spectrum


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