scholarly journals High-throughput characterisation of bull semen motility using differential dynamic microscopy

2018 ◽  
Author(s):  
Alys Jepson ◽  
Jochen Arlt ◽  
Jonathan Statham ◽  
Mark Spilman ◽  
Katie Burton ◽  
...  
Keyword(s):  
High Throughput ◽  
Oscillation Amplitude ◽  
Field Of View ◽  
Large Field ◽  
Laboratory Measurements ◽  
Spermatozoa Motility ◽  
Bull Semen ◽  
The Mean ◽  
On Farm ◽  
Low Cell Density

AbstractWe report a high-throughput technique for characterising the motility of spermatozoa using differential dynamic microscopy. A large field of view movie (~ 10mm2) records thousands of cells (e.g. ≈ 5000 cells even at a low cell density of 20 × 106 cells/ml) at once and yields averaged measurements of the mean (υ) and standard deviation (σ) of the swimming speed, a head oscillation amplitude (A0) and frequency (f0), and the fraction of motile spermatozoa (α). Interestingly, the measurement of α relies on the swimming spermatozoa enhancing the motion of the non-swimming population. We demonstrate the ease and rapidity of our method by performing on-farm characterisation of bull spermatozoa motility, and validate the technique by comparing laboratory measurements with tracking. Our results confirm the long-standing theoretical prediction that for swimming spermatozoa.

High-throughput and uniform large field-of-view multichannel fluorescence microscopy with super-thin dichroism for a dPCR gene chip

2020 ◽  
Vol 59 (34) ◽  
pp. 10768
Author(s):  
Francis Yaw Otuboah ◽  
Jihong Zheng ◽  
Cheng Chen ◽  
Zicheng Wang ◽  
Xinjun Wan ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
High Throughput ◽  
Gene Chip ◽  
Field Of View ◽  
Large Field

Sentinel Node Detection with Imaging Probe

2002 ◽  
Vol 88 (3) ◽  
pp. S32-S35 ◽  
Author(s):  
O Schillaci ◽  
G D'Errico ◽  
R Scafè ◽  
A Soluri ◽  
N Burgio ◽  
...  
Keyword(s):  
Orthopedic Surgery ◽  
Gamma Camera ◽  
Field Of View ◽  
Large Field ◽  
Sentinel Node Detection ◽  
Subjective Impression ◽  
Imaging Probe ◽  
Multicenter Trials ◽  
The Mean ◽  
Cooperative Project

A one-square-inch-field-of-view mini gamma camera, whose first prototype was built by us in 1998 and given the name imaging probe (IP), was initially employed in sentinel lymph node (SLN) detection. This is probably the best way of learning how to use it. In the present work IP was used for SLN localization by a medical team that, after having been trained by the group of nuclear physicians of “La Sapienza” University who designed and first used the detector, used IP at their own hospital to 1) acquire experience for future use during surgery (a cooperative project on IP-radioguided orthopedic surgery is ongoing) and 2) start multicenter trials with IP. The SLN was identified and localized with IP and a non-imaging probe, Neoprobe 2000, in six patients with breast cancer who underwent lymphoscintigraphy for SLN biopsy. The operators who used Neoprobe and IP were blinded to each other's findings and to the results obtained with the large-field-of-view Anger camera that was used for lymphoscintigraphy. The Anger camera, IP and Neoprobe detected seven SLNs in six patients. The mean detection time was 2 mins 6 s (standard deviation (SD) 26 s) with IP, and 2 mins 18 s (SD 47 s) with Neoprobe 2000. The SLN that was most difficult to find was detected in 2 mins 56 s with IP and 3 mins 45 s with Neoprobe. The operators' subjective impression of having detected the SLN was “absolutely sure” for 7/7 nodes with IP and “absolutely sure” for 5/7 nodes with Neoprobe.

Design of a reduced objective Lens fluorescence dPCR Gene chip detection system with high-throughput and large field of view

Optik ◽  
2019 ◽  
Vol 179 ◽  
pp. 1071-1083
Author(s):  
Francis Yaw Otuboah ◽  
Zheng Jihong ◽  
Zhu Tianyun ◽  
Chen Cheng
Keyword(s):  
High Throughput ◽  
Detection System ◽  
Gene Chip ◽  
Field Of View ◽  
Large Field ◽  
Objective Lens

Multiple beam ptychography for large field-of-view, high throughput, quantitative phase contrast imaging

2018 ◽  
Vol 184 ◽  
pp. 164-171 ◽  
Author(s):  
Charles Bevis ◽  
Robert Karl ◽  
Jonathan Reichanadter ◽  
Dennis F. Gardner ◽  
Christina Porter ◽  
...  
Keyword(s):  
High Throughput ◽  
Phase Contrast ◽  
Field Of View ◽  
Phase Contrast Imaging ◽  
Large Field ◽  
Contrast Imaging ◽  
Quantitative Phase ◽  
Multiple Beam

scholarly journals Intraoperative Scintigraphy Using a Large Field-of-View Portable Gamma Camera for Primary Hyperparathyroidism: Initial Experience

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Nathan C. Hall ◽  
Robert L. Plews ◽  
Amit Agrawal ◽  
Stephen P. Povoski ◽  
Chadwick L. Wright ◽  
...  
Keyword(s):  
Parathyroid Adenoma ◽  
Gamma Camera ◽  
Field Of View ◽  
Large Field ◽  
Time Saving ◽  
Valuable Adjunct ◽  
Parathyroid Hormone Monitoring ◽  
Parathyroid Adenomas ◽  
Portable Gamma Camera ◽  
The Mean

Background.We investigated a novel technique, intraoperative99 mTc-Sestamibi (MIBI) imaging (neck and excised specimen (ES)), using a large field-of-view portable gamma camera (LFOVGC), for expediting confirmation of MIBI-avid parathyroid adenoma removal.Methods.Twenty patients with MIBI-avid parathyroid adenomas were preoperatively administered MIBI and intraoperatively imaged prior to incision (neck) and immediately following resection (neck and/or ES). Preoperative and intraoperative serum parathyroid hormone monitoring (IOPTH) and pathology (path) were also performed.Results.MIBI neck activity was absent and specimen activity was present in 13/20 with imaging after initial ES removal. In the remaining 7/20 cases, residual neck activity and/or absent ES activity prompted excision of additional tissue, ultimately leading to complete hyperfunctioning tissue excision. Postexcision LFOVGC ES imaging confirmed parathyroid adenoma resection 100% when postresection imaging qualitatively had activity (ES) and/or no activity (neck). The mean ± SEM time saving using intraoperative LFOVGC data to confirm resection versus first IOPTH or path result would have been 22.0 ± 2 minutes (specimen imaging) and 26.0 ± 3 minutes (neck imaging).Conclusion.Utilization of a novel real-time intraoperative LFOVGC imaging approach can provide confirmation of MIBI-avid parathyroid adenoma removal appreciably faster than IOPTH and/or path and may provide a valuable adjunct to parathyroid surgery.

Ratio imaging of intracellular ion concentration

1992 ◽  
Vol 50 (2) ◽  
pp. 1160-1161
Author(s):  
Stephen R. Bolsover
Keyword(s):  
Individual Cell ◽  
Video Camera ◽  
Field Of View ◽  
Ion Concentration ◽  
Fluorescence Signal ◽  
Ion Imaging ◽  
Ratiometric Fluorescence ◽  
Ratio Imaging ◽  
The Mean ◽  
The Many

The field of intracellular ion concentration measurement expanded greatly in the 1980's due primarily to the development by Roger Tsien of ratiometric fluorescence dyes. These dyes have many applications, and in particular they make possible to image ion concentrations: to produce maps of the ion concentration within living cells. Ion imagers comprise a fluorescence microscope, an imaging light detector such as a video camera, and a computer system to process the fluorescence signal and display the map of ion concentration.Ion imaging can be used for two distinct purposes. In the first, the imager looks at a field of cells, measuring the mean ion concentration in each cell of the many in the field of view. One can then, for instance, challenge the cells with an agonist and examine the response of each individual cell. Ion imagers are not necessary for this sort of experiment: one can instead use a system that measures the mean ion concentration in a just one cell at any one time. However, they are very much more convenient.

Numerical analysis of shift error in X-ray phase contrast imaging for large field of view

2017 ◽  
Vol 34 (1) ◽  
pp. 8
Author(s):  
Jianheng Huang ◽  
Yaohu Lei ◽  
Xin Liu ◽  
Jinchuan Guo ◽  
Ji Li ◽  
...  
Keyword(s):  
Numerical Analysis ◽  
Phase Contrast ◽  
Field Of View ◽  
Phase Contrast Imaging ◽  
Large Field ◽  
Contrast Imaging ◽  
X Ray

Large Field-of-View Super-Resolution Optical Microscopy Based on Planar Polymer Waveguides

ACS Photonics ◽  
2021 ◽  
Author(s):  
Anders Kokkvoll Engdahl ◽  
Stefan Belle ◽  
Tung-Cheng Wang ◽  
Ralf Hellmann ◽  
Thomas Huser ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Super Resolution ◽  
Field Of View ◽  
Large Field ◽  
Polymer Waveguides
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